Primary Renal Cell Carcinoma in a Transplanted Kidney: Genetic Evidence of Recipient Origin

Boix, Rodolfo Rubén Salassa; Sanz, Carolina; M, Mora; Quer, Ariadna; Beyer, Katrin; Musulén, Eva; González, Carlos; Bayona, Salvador; Saladie, J.M.; Ariza, Aurelio

doi:10.1097/tp.0b013e31819d1e5f

Cited by 34 publications

(30 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Immunohistochemistry and hematoxilin-eosin staining on formalin-fixed paraffin-embedded tissue sections were performed as described (23). For immunohistochemistry, we have optimized the signal background for anti-PADI2 (Proteintech UK; reference number 12110-1-AP) using positive and negative control samples.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Downregulation of the Deiminase PADI2 Is an Early Event in Colorectal Carcinogenesis and Indicates Poor Prognosis

Cantariño

Musulén

Valero

et al. 2016

Molecular Cancer Research

View full text Add to dashboard Cite

Peptidyl arginine deiminases (PADI) are a family of enzymes that catalyze the poorly understood posttranslational modification converting arginine residues into citrullines. In this study, the role of PADIs in the pathogenesis of colorectal cancer was investigated. Specifically, RNA expression was analyzed and its association with survival in a cohort of 98 colorectal cancer patient specimens with matched adjacent mucosa and 50 controls from donors without cancer. Key results were validated in an independent collection of tumors with matched adjacent mucosa and by mining of a publicly available expression data set. Protein expression was analyzed by immunoblotting for cell lines or IHC for patient specimens that further included 24 cases of adenocarcinoma with adjacent dysplasia and 11 cases of active ulcerative colitis. The data indicate that PADI2 is the dominantly expressed PADI enzyme in colon mucosa and is upregulated during differentiation. PADI2 expression is low or absent in colorectal cancer. Frequently, this occurs already at the stage of low-grade dysplasia. Mucosal PADI2 expression is also low in ulcerative colitis. The expression level of PADI2 in tumor and adjacent mucosa correlates with differential survival: low levels associate with poor prognosis.Implications: Downregulation of PADI2 is an early event in the pathogenesis of colorectal cancer associated with poor prognosis and points toward a possible role of citrullination in modulating tumor cells and their microenvironment.

show abstract

Section: Immunohistochemistrymentioning

confidence: 99%

Downregulation of the Deiminase PADI2 Is an Early Event in Colorectal Carcinogenesis and Indicates Poor Prognosis

Cantariño

Musulén

Valero

et al. 2016

Molecular Cancer Research

View full text Add to dashboard Cite

show abstract

“…Lysis and Western blot analyses were performed as previously described (9,42). Immunohistochemistry and immunofluorescence on paraffin-embedded sections were performed as described previously (5,24). For the separation of nucleosolic and chromatin fractions, we have used a modified version of the Dignam protocol.…”

mentioning

confidence: 99%

MacroH2A1 Regulates the Balance between Self-Renewal and Differentiation Commitment in Embryonic and Adult Stem Cells

Creppe

Janich

Cantariño

et al. 2012

Molecular and Cellular Biology

100

View full text Add to dashboard Cite

e One of the most striking epigenetic alterations that occurs at the level of the nucleosome is the complete exchange of the canonical H2A histones for the macroH2A variant. Here, we provide insight into the poorly recognized function of macroH2A in transcriptional activation and demonstrate its relevance in embryonic and adult stem cells. Knockdown of macroH2A1 in mouse embryonic stem (mES) cells limited their capacity to differentiate but not their self-renewal. The loss of macroH2A1 interfered with the proper activation of differentiation genes, most of which are direct target genes of macroH2A. Additionally, macroH2A1-deficient mES cells displayed incomplete inactivation of pluripotency genes and formed defective embryoid bodies. In vivo, macroH2A1-deficient teratomas contained a massive expansion of malignant, undifferentiated carcinoma tissue. In the heterogeneous culture of primary human keratinocytes, macroH2A1 levels negatively correlated with the self-renewal capacity of the pluripotent compartment. Together these results establish macroH2A1 as a critical chromatin component that regulates the delicate balance between self-renewal and differentiation of embryonic and adult stem cells.

show abstract

“…Once RCC in the transplanted kidney is diagnosed, it is crucial to determine the genetic origin of the tumor by means of DNA analysis. Thus the potential transmission of tumor cells to other recipients from the same donor can be assessed (Boix, et al, 2009). There is no consensus on treatment of RCC in the transplanted kidney.…”

Section: Comments On Partial Nephrectomy For Transplanted Kidneysmentioning

confidence: 99%

Novel Renal Transplant-Related Surgical Approaches in the 21st Century

Chueh¹,

Sankari²

2011

Understanding the Complexities of Kidney Transplantation

View full text Add to dashboard Cite

Primary Renal Cell Carcinoma in a Transplanted Kidney: Genetic Evidence of Recipient Origin

Abstract: We report the first case of a grafted-kidney RCC whose recipient's cell origin has been proved by microsatellite analysis. An origin from the recipient's kidney or bone marrow stem cells is proposed as the more plausible hypothesis.

Cited by 34 publications

References 23 publications

Downregulation of the Deiminase PADI2 Is an Early Event in Colorectal Carcinogenesis and Indicates Poor Prognosis

Downregulation of the Deiminase PADI2 Is an Early Event in Colorectal Carcinogenesis and Indicates Poor Prognosis

MacroH2A1 Regulates the Balance between Self-Renewal and Differentiation Commitment in Embryonic and Adult Stem Cells

Novel Renal Transplant-Related Surgical Approaches in the 21st Century

Contact Info

Product

Resources

About