1994
DOI: 10.1089/hum.1994.5.10-1203
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Primary Fibroblasts from Human Adults as Target Cells forEx VivoTransfection and Gene Therapy

Abstract: Diploid fibroblast (dFb) cultures were established from a total of 106 skin and serosa biopsies of human adults. Using an optimized enzymatic dissociation procedure, 10(11) dFb/cm2 skin were obtained from patients younger than 60 years after an average time of 89 +/- 8 days, with a mean population doubling time of 3.87 +/- 1.4 days. Enzymatic dissociation of skin biopsies yielded cultures of significantly higher growth capacity of dFb than those prepared by mechanical dissociation followed by spontaneous outgr… Show more

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Cited by 46 publications
(33 citation statements)
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“…As described in previous studies, telomeres were significantly shorter in latepassage cells of all three cell lines than in early-passage cells, but telomere length was stabilized by the overexpression of TERT ( Fig. 1; data not shown for WI38 cells) (21,27,34,36,56).…”
Section: Resultssupporting
confidence: 79%
See 1 more Smart Citation
“…As described in previous studies, telomeres were significantly shorter in latepassage cells of all three cell lines than in early-passage cells, but telomere length was stabilized by the overexpression of TERT ( Fig. 1; data not shown for WI38 cells) (21,27,34,36,56).…”
Section: Resultssupporting
confidence: 79%
“…To analyze whether the senescence phenotype induced by telomere shortening is constitutively operative at the same level or is amplified by mitogenic stimuli, we first explored this phenomenon in human fibroblast cultures with three different cell lines (IMR90, WI38, and HK1) (27,36,56). As described in previous studies, telomeres were significantly shorter in latepassage cells of all three cell lines than in early-passage cells, but telomere length was stabilized by the overexpression of TERT ( Fig.…”
Section: Resultsmentioning
confidence: 77%
“…They reached the stationary phase after 96 hours and the population doubling time was about 24 hours. Results obtained by other authors were quite diverse and they were dependent on the species origin and age of the tissue donor (Li et al 2009;Wu et al 2008;Veelken et al 1994).…”
Section: Resultsmentioning
confidence: 97%
“…Cell culture U-2-OS human osteosarcoma cell lines and primary human fibroblasts (Veelken et al, 1994) were cultured in 90% Dulbecco's modified Eagle's medium (DMEM, Invitrogen, Carlsbad, CA, USA)/10% fetal bovine serum (FBS) supplemented with 1% penicillin/streptomycin (Invitrogen), 1 mM sodium pyruvate (Invitrogen) and 2 mM L-glutamate (Invitrogen). Transduced U-2-OS and primary human fibroblasts were grown in the presence of 2.5 mg/ml puromycin (Sigma, St Louis, MO, USA).…”
Section: Plasmidsmentioning
confidence: 99%