Primary culture of cortical neurons, type-1 astrocytes, and microglial cells from cynomolgus monkey (Macaca fascicularis) fetuses

Negishi, Takayuki; Ishii, Yoshiyuki; Kyuwa, Shigeru; Kuroda, Yoichiro; Yoshikawa, Yasuhiro

doi:10.1016/j.jneumeth.2003.08.006

Cited by 21 publications

(13 citation statements)

References 38 publications

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“…Primary astrocytes are typically generated from human and rodent fetal brain tissues and have been used both as the supportive layers in mixed neuronal-astrocytic co-cultures (Kivell et al, 2002;Negishi et al, 2003). Astrocytes are found essential for optimal growth and maturation of human neurons (Hartley et al, 1999;Le and Esquenazi, 2002;Martinez and Gomes, 2005;Muller and Seifert, 1982).…”

Section: Discussionmentioning

confidence: 99%

Inducible functional expression of Bcl-2 in human astrocytes derived from NTera-2 cells

Ozdener

2007

Journal of Neuroscience Methods

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Section: Discussionmentioning

confidence: 99%

Inducible functional expression of Bcl-2 in human astrocytes derived from NTera-2 cells

Ozdener

2007

Journal of Neuroscience Methods

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“…Oligodendrocytes were purified and cultured from mixed glial cell cultures as described (Giulian and Baker, 1986; Negishi et al, 2003). Briefly, the mixed glial cells prepared from p1~p2 rat/mouse postnatal pups were plated onto the poly-L-lysine coated flasks and cultured in the DMEM+10% FBS+penicillin/streptomycin (DMEM-FBS) for 15–20 days.…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective Role of Haptoglobin after Intracerebral Hemorrhage

Zhao¹,

Shen²,

Sun³

et al. 2009

J. Neurosci.

143

169

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After intracerebral hemorrhage (ICH), the brain parenchyma is exposed to blood containing red blood cells (RBCs) and consequently to its lysis products. Iron-rich hemoglobin (Hb) is the most abundant protein in RBCs. When released into the brain parenchyma during hemolysis, Hb becomes a central mediator of cytotoxicity. Our study indicates that haptoglobin (Hp), an acute-phase response protein primarily synthesized in the liver and known to bind and neutralize Hb in the bloodstream, is also expressed in brain in which it plays an important role in defending neurons from damage induced by hemolytic products after ICH. We demonstrate that the Hb-induced hypohaptoglobinemia aggravates ICH-induced brain damage while pharmacologic intervention with sulforaphane to induce brain Hp is linked to a reduction in brain damage. In agreement with these findings, Hp deficiency worsens whereas Hp overexpression alleviates ICH-mediated brain injury. We also identified that oligodendroglia are the primary source of brain-derived Hp among brain cells and that oligodendroglia-released Hp plays protective roles against Hb-mediated toxicity to neurons and oligodendrocytes. We conclude that Hp, particularly the brain-derived Hp, plays cytoprotective roles and represents a potential therapeutic target for ICH treatment.

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“…Cells were also plated at 4.2 × 10 5 cells/cm 2 in CM onto uncoated culture dishes for semiquantitative RT-PCR and Western Blotting experiments. A half volume of culture supernatant was replaced with prewarmed CM once per week (Negishi et al, 2003).…”

Section: Rat Astrocyte Culturesmentioning

confidence: 99%

Aβ Upregulates and Colocalizes with LGI3 in Cultured Rat Astrocytes

et al. 2007

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1. The leucine-rich glioma inactivated (LGI) family of genes encodes a leucine-rich repeat (LRR) protein, proteins that are thought to be specifically involved in protein-protein and protein-matrix interactions. Since amyloid beta peptide (Abeta) has been previously shown to induce the expression of another LRR-encoding gene in neural cells, we assessed how Abeta affects LGI gene expression in rat primary cerebral cortical cultures and astrocyte cultures. Both RT-PCR and Western Blotting analyses revealed that Abeta robustly induced the expression of LGI3 in rat astrocyte cultures.2. Western Blotting analyses also showed that both glial fibrillary acidic protein (GFAP) and apolipoprotein E (ApoE) significantly increased coincidentally with the Abeta-induced upregulation of LGI3. Immunocytochemistry showed that LGI3 colocalized with Abeta at plasma membranes and also with internalized Abeta in astrocytes. These findings suggest that activated LGI3 may be involved in the astroglial response against Abeta.

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Primary culture of cortical neurons, type-1 astrocytes, and microglial cells from cynomolgus monkey (Macaca fascicularis) fetuses

Cited by 21 publications

References 38 publications

Inducible functional expression of Bcl-2 in human astrocytes derived from NTera-2 cells

Inducible functional expression of Bcl-2 in human astrocytes derived from NTera-2 cells

Neuroprotective Role of Haptoglobin after Intracerebral Hemorrhage

Aβ Upregulates and Colocalizes with LGI3 in Cultured Rat Astrocytes

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