Primary culture macrophages from fish as potential experimental model in toxicity studies with multiwalled carbon nanotubes

Abstract

Multiwalled carbon nanotube (MWCNT) has been broadly used in several sectors of society. This material when exposed to the environment might reach the aquatic animals and cause toxic effects. Here, it was evaluated the MWCNTs toxicity in melanomacrophages primary culture that was submitted to 1 µ gm L-1 MWCNTs for 24 hours. After exposition to MWCNT, 48 and 59% liver and spleen melanomacrophages were healthy, respectively. The control group presented 85% viability. Phagocytosis activity of melanomacrophages was observed by presence of black inclusions in cytoplasm. The findings indicate MWCNT was cytotoxic to melanomacrophages, where its release and effect into aquatic environment must be more studied. Finally, the melanomacrophages present large potential as experimental model for evaluation of carbon-based nanomaterial toxicity.

Toxic Effects of Multiwalled Carbon Nanotubes on the Zebrafish (Danio rerio) and the Brine Shrimp (Artemia salina): a Morphological, Histological, and Immunohistochemical Study

“…It was dispersed in water plus sonification, following the manufacturer's recommendations. The chemical characterization of MWCNTs can be found in our previous work [28].…”