Primary Cell Cultures from Human Renal Cortex and Renal-Cell Carcinoma Evidence a Differential Expression of Two Spliced Isoforms of Annexin A3

Bianchi, Cristina; Bombelli, S; Raimondo, Francesca; Torsello, B; Angeloni, Valentina; Ferrero, Stefano; Stefano, Vitalba Di; Chinello, Clizia; Cifola, Ingrid; Invernizzi, L; Brambilla, Paolo; Magni, Fulvio; Pitto, Marina; Zanetti, G.; Mocarelli, Paolo; Perego, R

doi:10.2353/ajpath.2010.090402

Cited by 45 publications

(70 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These cultures represent a reproducible and well-characterized cellular model that maintains, in the first passages, the phenotypic and molecular characteristics of the corresponding tissue (Perego et al, 2005;Bianchi et al, 2010;Cifola et al, 2011). Our data showed that these primary cells, grown in high-glucose medium, acquired an elongated morphology but only a few molecular EMT features, and did not reach a full EMT phenotype.…”

Section: Discussionmentioning

confidence: 83%

“…Immunofluorescence and stress fiber analysis Cells were seeded on glass coverslips, fixed and incubated with the indicated primary antibodies and, when necessary, with secondary antibodies (Table S1), as previously described (Bianchi et al, 2010). Stress fibers were labeled by Alexa-Fluor-594-phalloidin (dilution 1:100; Molecular Probes, Carlsberg, CA).…”

Section: Human Primary Tubular Cell Cultures and Treatmentsmentioning

confidence: 99%

“…Primary tubular cell cultures were obtained and characterized as previously described (Bianchi et al, 2010) from 30 different renal cortex tissue specimens. The cells, trypsinized at the first confluence, were diluted and replated to reach the second confluence at the end of each treatment point.…”

Section: Human Primary Tubular Cell Cultures and Treatmentsmentioning

confidence: 99%

“…Arg is also involved in some aspects of the EMT process, like cell migration and cytoskeleton modulation through the RhoA-ROCK pathway (Hernández et al, 2004;Peacock et al, 2007;Bianchi et al, 2013), which is also involved in TGF-β secretion in a high-glucose-treated HK-2 cell line (Gu et al, 2013). Based on these data, in the current study we analyzed the involvement of Arg tyrosine kinase in the production of TGF-β1 induced in well-characterized human primary tubular cell cultures (Perego et al, 2005;Bianchi et al, 2010;Cifola et al, 2011) by treatment with high glucose, which also induced some phenotypical and molecular changes in these tubular cells.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Arg tyrosine kinase modulates TGF-β1 production in human renal tubular cells under high-glucose conditions

Torsello

Bianchi

Meregalli

et al. 2016

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

Renal tubular cells are involved in the tubular interstitial fibrosis observed in diabetic nephropathy. It is debated whether epithelialmesenchymal transition (EMT) affects tubular cells, which under highglucose conditions overproduce transforming growth factor-β (TGF-β), a fibrogenic cytokine involved in interstitial fibrosis development. Our study investigated the involvement of non-receptor tyrosine kinase Arg (also called Abl2) in TGF-β production. Human primary tubular cell cultures exposed to high-glucose conditions were used. These cells showed an elongated morphology, stress fibers and vimentin increment but maintained most of the epithelial marker expression and distribution. In these cells exposed to high glucose, which overexpressed and secreted active TGF-β1, Arg protein and activity was downregulated. A further TGF-β1 increase was induced by Arg silencing with siRNA, as with the Arg tyrosine kinase inhibitor Imatinib. In the cells exposed to high glucose, reactive oxygen species (ROS)-dependent Arg kinase downregulation induced both RhoA activation, through p190RhoGAPA (also known as ARHGAP35) modulation, and proteasome activity inhibition. These data evidence a new specific involvement of Arg kinase into the regulation of TGF-β1 expression in tubular cells under high-glucose conditions and provide cues for new translational approaches in diabetic nephropathy.

show abstract

Section: Discussionmentioning

confidence: 83%

Section: Human Primary Tubular Cell Cultures and Treatmentsmentioning

confidence: 99%

Section: Human Primary Tubular Cell Cultures and Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Arg tyrosine kinase modulates TGF-β1 production in human renal tubular cells under high-glucose conditions

Torsello

Bianchi

Meregalli

et al. 2016

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Annexin A3 may act as a tumor suppressor or tumor promoter depending upon the type of tumor cell and tissue. Annexin A3 has been shown to be upregulated [13] in prostate cancer, and downregulated in kidney and thyroid cancers [14,15]. Increased expression of Annexin A3 enhanced the drug resistance of ovarian and liver cancers [16,19], promoted the progression of pancreatic, rectal, and glandular cancers [17], and accelerated the metastasis of lung cancer [18].…”

mentioning

confidence: 99%

Impact of Annexin A3 expression in gastric cancer cells

Yu¹,

Y²,

Fan³

et al. 2014

neo

View full text Add to dashboard Cite

Annexin A3 participates in various biological processes, including tumorigenesis, drug resistance, and metastasis. The aim of this study was to investigate the expression of Annexin A3 in gastric cancer and its relationship with cell differentiation, migration, and invasion of gastric cancer cells. Annexin A3 expression in gastric cancer tissues was detected by quantitative real-time PCR and Western blotting. The proliferation of gastric cancer cells was measured by the MTT assay. Cell migration and invasion were determined via wound healing and transwell assays, respectively. Knock down of endogenous Annexin A3 in gastric cancer BGC823 cells was performed using siRNA technology. The expression of Annexin A3 was significantly upregulated in gastric cancer tissues, and negatively correlated with the differentiation degree. Silencing of endogenous Annexin A3 suppressed the proliferation, migration, and invasion of BGC823 cells. Additionally, the expression of p21, p27, TIMP-1, and TIMP-2 was upregulated, and the expression of PCNA, cyclin D1, MMP-1, and MMP-2 decreased in cells treated with Annexin A3-siRNA. Annexin A3 was upregulated in gastric cancer cells. Deletion of endogenous Annexin A3 significantly inhibited gastric cancer cell proliferation, migration, and invasion.

show abstract

The cross‐talk between Abl2 tyrosine kinase and TGFβ1 signalling modulates the invasion of clear cell Renal Cell Carcinoma cells

et al. 2022

Self Cite

View full text Add to dashboard Cite

Clear cell Renal Cell Carcinoma (ccRCC) is the most common and metastatic urological cancer. Molecular players of ccRCC progression and metastasis are not completely known. Here, using primary cell cultures from patients' specimens, we found that TGFb1/Smad signalling is more activated in high versus low grade ccRCC and inversely correlates with Abl2 tyrosine kinase protein expression. TGFb1 treatment increased ubiquitination and degradation of Abl2 protein in ccRCC cell lines by TGFb1/Smad pathway activation and reactive oxygen species production. 3D invasion and matrix degradation assays showed that Abl2 promoted TGFb1-induced ccRCC cell invasion and maturation of invadopodia, a hallmark of tumour invasion and metastasis. Our findings define Abl2 as a new downstream molecule of TGFb1 signalling and putative target to counteract advanced ccRCC.

show abstract

Primary Cell Cultures from Human Renal Cortex and Renal-Cell Carcinoma Evidence a Differential Expression of Two Spliced Isoforms of Annexin A3

Cited by 45 publications

References 48 publications

Arg tyrosine kinase modulates TGF-β1 production in human renal tubular cells under high-glucose conditions

Arg tyrosine kinase modulates TGF-β1 production in human renal tubular cells under high-glucose conditions

Impact of Annexin A3 expression in gastric cancer cells

The cross‐talk between Abl2 tyrosine kinase and TGFβ1 signalling modulates the invasion of clear cell Renal Cell Carcinoma cells

Contact Info

Product

Resources

About