Primary bone-derived cells induce osteogenic differentiation without exogenous factors in human embryonic stem cells

Ahn, Seong Eun; Kim, Sinae; Park, Kyu Hyung; Moon, Sung; Lee, Hae Jin; Kim, Gi Jin; Lee, Young Jae; Park, Keun Hong; Yul, Kwang; Chung, Hyung Min

doi:10.1016/j.bbrc.2005.12.020

Cited by 98 publications

(69 citation statements)

References 29 publications

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“…Human embryonic stem cells (hESCs) have been shown to differentiate toward bone and cartilage lineages. [1][2][3] However, scientific challenges, immunologic issues, and ethical concerns motivate the examination of reservoirs of stem cells from postnatal tissues. These include, but are not limited to, cells isolated from the bone marrow, 4,5 the circulation or blood vessels, 6,7 adipose tissue, [8][9][10] human placenta, 11 human umbilical cord, 12,13 human amniotic fluid, 14 and skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

“…In the musculoskeletal field, this is largely due to the technical difficulties in generating highly efficacious engineered tissues, but it is also due to a lack of outcome measures to rigorously evaluate a novel construct in animals, and the lack of a favorable patient population for a cost-effective clinical trial. Tissue engineering of articular and meniscal cartilage provides an excellent example of these limitations for the following reasons: (1) little is known about what factors, if any, can truly restore damaged tissue to its native form; (2) large animals (i.e., goats, sheep) appear to be the only rigorous models; (3) while enormous, the patient population is young and healthy, and thus the risk:benefit assessment of a clinical trial is very unfavorable for highly innovative technologies (i.e., genetically modified stem cells, viral gene therapy); and (4) there are no quantitative outcome measures to scientifically prove that tissueengineered cartilage restores defects to their native form in humans. In contrast, tissue engineering of bone has many advantages including the following: (1) a wealth of available knowledge 17 ; (2) the ability to utilize acellular constructs that can remodel into live tissue in vivo; (3) a potential patient population with a favorable risk:benefit assessment (metastatic cancer patients undergoing limb-sparing surgery); and (4) definitive quantitative outcome measures in animals and humans.…”

Section: Introductionmentioning

confidence: 99%

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Regenerative medicine in orthopaedic surgery

Corsi

Schwarz

Mooney

et al. 2007

Journal Orthopaedic Research

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Regenerative medicine holds great promise for orthopaedic surgery. As surgeons continue to face challenges regarding the healing of diseased or injured musculoskeletal tissues, regenerative medicine aims to develop novel therapies that will replace, repair, or promote tissue regeneration. This review article will provide an overview of the different research areas involved in regenerative medicine, such as stem cells, bioinductive factors, and scaffolds. The potential use of stem cells for orthopaedic tissue engineering will be addressed by presenting the current progress with skeletal muscle-derived stem cells. As well, the development of a revascularized massive allograft will be described and will serve as a prototypic model of orthopaedic tissue engineering. Lastly, we will describe current approaches used to design cell instructive materials and how they can be used to promote and regulate the formation of bony tissue. ß

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regenerative medicine in orthopaedic surgery

Corsi

Schwarz

Mooney

et al. 2007

Journal Orthopaedic Research

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“…ESCs into the osteogenic lineage in a direct co-culture system [144]. In an alternative co-culture system, the use of bone was avoided.…”

Section: Osteogenic Differentiation Of Escs In a Co-culture Systemmentioning

confidence: 99%

“…Human ESCs which were co-cultured in vitro for 14 days on a feeder layer of PBDs as described above [144], were seeded onto porous composite scaffolds using fibrinogen and implanted in immuno-deficient mice [154]. After 4 and 8 weeks in vivo, small patches of bone-like tissue were observed between the fibrous connective tissue.…”

Section: Osteogenic Differentiation Of Escs In Vivomentioning

confidence: 99%

Skeletal tissue engineering using embryonic stem cells

Jukes

Blitterswijk

Boer

2010

J Tissue Eng Regen Med

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“…[49][50][51][52][53][54] Osteoblasts, osteoclasts, nerve cells, and vascular cells all contribute to bone architecture and function, and given the correct signals, hESCs will become these cell types. 48 hESCs are the superior choice for bone tissue regeneration strategies, but they offer obstacles to overcome as well.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Differentiation of Human Embryonic Stem Cells on Extracellular Matrix-Containing Osteomimetic Scaffolds for Bone Tissue Engineering

Rutledge

Cheng

Pryzhkova

et al. 2014

Tissue Engineering Part C: Methods

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Current methods of treating critical size bone defects include autografts and allografts, however, both present major limitations including donor-site morbidity, risk of disease transmission, and immune rejection. Tissue engineering provides a promising alternative to circumvent these shortcomings through the use of autologous cells, three-dimensional scaffolds, and growth factors. We investigated the development of a scaffold with native bone extracellular matrix (ECM) components for directing the osteogenic differentiation of human embryonic stem cells (hESCs). Toward this goal, a microsphere-sintering technique was used to fabricate poly(lactic-co-glycolic acid) (PLGA) scaffolds with optimum mechanical and structural properties. Human osteoblasts (hOBs) were seeded on these scaffolds to deposit bone ECM for 14 days. This was followed by a decellularization step leaving the mineralized matrix intact. Characterization of the decellularized PLGA scaffolds confirmed the deposition of calcium, collagen II, and alkaline phosphatase by osteoblasts. hESCs were seeded on the osteomimetic substrates in the presence of osteogenic growth medium, and osteogenicity was determined according to calcium content, osteocalcin expression, and bone marker gene regulation. Cell proliferation studies showed a constant increase in number for hESCs seeded on both PLGA and ECM-coated PLGA scaffolds. Calcium deposition by hESCs was significantly higher on the osteomimetic scaffolds compared with the control groups. Consistently, immunofluorescence staining demonstrated an increased expression of osteocalcin in hESCs seeded on ECM-coated osteomimetic PLGA scaffolds. Gene expression analysis of RUNX2 and osteocalcin further confirmed osteogenic differentiation of hESCs at the highest expression level on osteomimetic PLGA. These results together demonstrate the potential of PLGA scaffolds with native bone ECM components to direct osteogenic differentiation of hESCs and induce bone formation.

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Primary bone-derived cells induce osteogenic differentiation without exogenous factors in human embryonic stem cells

Cited by 98 publications

References 29 publications

Regenerative medicine in orthopaedic surgery

Regenerative medicine in orthopaedic surgery

Skeletal tissue engineering using embryonic stem cells

Enhanced Differentiation of Human Embryonic Stem Cells on Extracellular Matrix-Containing Osteomimetic Scaffolds for Bone Tissue Engineering

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