Primary Antituberculosis Drug Resistance at Turkish Military Chest Diseases Hospital in Istanbul

Kartaloğlu, Zafer; Bozkanat, Erkan; Ozturkeri, Hakan; Okutan, Oğuzhan; İlvan, Ahmet

doi:10.1159/000065815

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…In our study, about 23% of isolates were resistant to one or more drugs and about 4% of isolates were multidrug resistant (i.e., resistant to at least isoniazid and rifampin). This amount of drug resistance is consistent with published surveys [3][4][5][6]29] of drug-resistant tuberculosis in Turkey which reported that about one-third of all cases (20-26% of new cases and 40-50% of previously treated cases) were resistant to at least one anti-tuberculosis drug and 5-10% of all cases were multidrug resistant. Patient information was not collected in our study and future studies will be needed to collect such information to further define the pattern of drug-resistant tuberculosis in the areas surveyed.…”

Section: Discussionsupporting

confidence: 90%

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Molecular Genotyping of Mycobacterium tuberculosis Isolates from Turkey

Gencer¹,

Shinnick²

2005

American J. of Infectious Diseases

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To survey the circulating strains of Mycobacterium tuberculosis in Turkey, all clinical isolates (381 patients) recovered in laboratories in six cities during one-month periods were collected and typed by IS6110-DNA fingerprinting and spoligotyping. Drug susceptibilities were also determined. About 23% of the isolates were resistant to one or more drugs and about 4% were multidrug resistant (i.e., resistant to at least isoniazid and rifampin). IS6110-DNA fingerprints and spoligotypes were obtained from 368 and 374 strains, respectively. Of the 374 isolates spoligotyped, 87 (23%) displayed unique spoligotypes and 287 (77%) displayed one of 34 spoligotypes (2-77 isolates per pattern). The clustered spoligotypes included ones that matched spoligotypes of the T (37% of isolates), LAM (20%), Haarlem (8%) and Beijing (2%) families. Of the 368 isolates IS6110-typed, 232 (63%) displayed unique IS6110-fingerprint patterns and 136 (37%) displayed one of 35 patterns (2-34 isolates per pattern). When IS6110 fingerprinting and spoligotyping information were combined for the 381 isolates tested, 273 isolates (72%) displayed unique genotypes and 108 isolates (28%) displayed one of 34 genotypes (2-24 isolates per genotype). In summary, many different strains are circulating in Turkey with no single strain appearing to be dominant as has been observed in other areas of the world with high tuberculosis incidence.

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Section: Discussionsupporting

confidence: 90%

“…Drug-resistant tuberculosis is increasing and is a significant threat to tuberculosis control because there are few drugs effective against M. tuberculosis. In Turkey, 20-26% of new cases of tuberculosis are resistant to at least one antituberculosis drug and 3-10% of new cases are multidrug resistant [3][4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

Molecular Genotyping of Mycobacterium tuberculosis Isolates from Turkey

Gencer¹,

Shinnick²

2005

American J. of Infectious Diseases

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“…The prevalence of multidrug‐resistant (MDR) Mycobacterium tuberculosis (defined as isolates that are non‐susceptible to isoniazid and rifampicin) is relatively low [32]; the reported figures vary between 2% and 21% [33–36]. According to the ‘Fight against Tuberculosis 2009 Report’ of the Turkish Ministry of Health (MoH), the frequency of MDR strains was 3% in new cases, whereas it was reported to be 18% in previously treated patients.…”

Section: Bacterial Infectionsmentioning

confidence: 99%

Leading infectious diseases problems in Turkey

Erdem

Akova

2012

Clinical Microbiology and Infection

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Turkey has significant geographical and socio-economic differences throughout a vast area of the country. These characteristics affect the epidemiology of infectious diseases, some of which are rarely seen in western Europe. However, effectively implemented control measures have resulted in decreased rates of many community-acquired infections, including tuberculosis and malaria, that were major health problems only a few decades ago. There are high rates of antimicrobial resistance in various nosocomial isolates of Gram-positive and Gram-negative bacteria. A recently implemented, nationwide, electronic resistance surveillance system in hospitals is expected to produce reliable data, and possibly will help to develop an effective strategy to decrease antimicrobial resistance in bacteria that currently plague many tertiary-care hospitals in the country. This article summarizes the most frequently encountered community-acquired infections, and gives an overview of current antimicrobial resistance in both outpatient and hospital settings in Turkey.

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“…25 The reported rate of resistance to at least one drug has been 23.8-39.2% in recent years. 1,3,6,8,20 However, so far, only a limited number of studies have been carried out to characterize the genetic changes associated with drug resistance of M. tuberculosis isolates obtained from Turkey. 2,19 In this report, we present this first investigation to profile genetic mutations associated with the RIF and INH resistance of M. tuberculosis clinical isolates obtained from TB patients from Malatya, a city located in Eastern Turkey with about 850,000 inhabitants and having a TB incidence rate (32/100,000 in 2000)…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Isoniazid and Rifampin Resistance ofMycobacterium tuberculosisClinical Isolates from Malatya, Turkey

Aktaş

Durmaz

Yang

et al. 2005

Microbial Drug Resistance

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Molecular characterization of drug resistance of Mycobacterium tuberculosis strains of different origins can generate information useful for developing molecular methods that are widely applicable for rapid drug resistance detection. Using DNA sequencing and allele-specific polymerase chain reaction (AS-PCR), we investigated genetic mutations associated with isoniazid (INH) and rifampin (RIF) resistance among 29 drug-resistant clinical isolates of M. tuberculosis collected from Malatya, Turkey, including 19 multi-drug-resistant (MDR) isolates. Point mutations were detected at codons 531, 516, 526, and 513 of the RNA polymerase beta- subunit gene (rpoB) in 10 (47.6%), five (23.8%), three (14.3%), and three (14.3%) of the 21 RIF-resistant isolates, respectively. Of the five isolates having mutations in codon 516, three also had mutations at codon 527; one had a concurrent mutation at codon 572. Mutations at codon 315 of the catalase-peroxidase-encoding gene (katG) were found in 17 (63.0%) of the 27 INH-resistant isolates. Interestingly, the katG codon 315 mutation was observed at a much higher frequency in MDR isolates than in INH-mono-resistant isolates ( approximately 79% vs. 25%). This study provided the first molecular characterization of INH and RIF resistance of M. tuberculosis clinical isolates from Eastern Turkey, and extended our knowledge of molecular basis of M. tuberculosis drug resistance.

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Primary Antituberculosis Drug Resistance at Turkish Military Chest Diseases Hospital in Istanbul

Cited by 10 publications

References 22 publications

Molecular Genotyping of Mycobacterium tuberculosis Isolates from Turkey

Molecular Genotyping of Mycobacterium tuberculosis Isolates from Turkey

Leading infectious diseases problems in Turkey

Molecular Characterization of Isoniazid and Rifampin Resistance ofMycobacterium tuberculosisClinical Isolates from Malatya, Turkey

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