Primary and secondary structures of<i>Tetrahymena</i>and aphid 5.8S rRNAs: Structural features of 5.8S rRNA which interacts with the 28S rRNA containing the hidden break

Fujiwara, Haruhiko; Ishikawa, Hajime

doi:10.1093/nar/10.17.5173

Cited by 20 publications

(9 citation statements)

References 28 publications

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“…This was supplemented with sequences not included in the compilation: a gnat (23), a thermophilic fungus (24), and the silkworm Bombyx (25). We also utilized sequences which have appeared subsequent to the compilation: Tetrahbrena and an aphid (26), the silkworm Philob1-i& (27), the slime mold Physarum (28), yellow lupine (29), a snail and sponge (17) and a sea urchin (30). Finally, we took into account species for which sequence corrections have appeared: the frog Xenops laevis (31), wheat (32), mouse (33) and brine shrimp (34).…”

Section: Materials and Methods Selection Of Representative 58s Rrnasmentioning

confidence: 99%

A universal model for the secondary structure of S.8S ribosomal RNA molecules, their contact sites with 28S ribosomal RNAs, and their prokaryotic equivalent

et al. 1984

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The phylogenetic approach (ref. 1) has been utilized in construction of a universal 5.8S rRNA secondary structure model, in which about 65% of the residues exist in paired structures. Conserved nucleotides primarily occupy unpaired regions. Multiple compensating base changes are demonstrated to be present in each of the five postulated helices, thereby forming a major basis for their proof. The results of chemical and enzymatic probing of 5.8S rRNAs (ref. 13, 32) are fully consistent with, and support, our model. This model differs in several ways from recently proposed 5.8S rRNA models (ref. 3, 4), which are discussed. Each of the helices in our model has been extended to the corresponding bacterial, chloroplast and mitochondrial sequences, which are demonstrated to be positionally conserved by alignment with their eukaryotic counterparts. This extension is also made for the base paired 5.8S/28S contact points, and their prokaryotic and organelle counterparts. The demonstrated identity of secondary structure in these diverse molecules strongly suggests that they perform equivalent functions in prokaryotic and eukaryotic ribosomes.

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Section: Materials and Methods Selection Of Representative 58s Rrnasmentioning

confidence: 99%

A universal model for the secondary structure of S.8S ribosomal RNA molecules, their contact sites with 28S ribosomal RNAs, and their prokaryotic equivalent

et al. 1984

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“…The sequence of 5.8S RNA has been reported for many species (19,(23)(24)(25)(26)(27). Generally, its size is 160 ±?…”

Section: 8s Mmentioning

confidence: 99%

Nucleotide sequence of the 18S–26S rRNA intergene region of the sea urchin

Hindenach¹,

Stafford²

1984

Nucl Acids Res

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The DNA sequence which spans the internal transcribed spacers of a cloned ribosomal transcription unit from the sea urchin, Lyteehinus variegatus, has been determined. The region extends from the conserved Eco RI site near the 3' end of the 18S rDNA to a Bam HI site in the 26S rDNA and includes 232 nucleotides coding for 18S rRNA, 367 nucleotides of internal transcribed spacer, 159 nucleotides coding for 5.8S rRNA, 338 nucleotides of internal transcribed spacer, and 505 nucleotides coding for 26S rRNA. The rRNA coding regions were identified by direct analysis of 3'-labeled 18S and 5.8S rRNA and 5'-labeled 5. . INTBODUCTLIQNEukaryotic ribosomal RNA is synthesized as a large precursor containing 17/18S, 5.8S, and 26/28S rRNAs plus spacer sequences. The RNA is posttranscriptionally modified and cleaved at specific sites to produce a set of intermediate precursors and finally the mature rRNAs (1,2). Interest in the nature of these processing sites has led investigators to sequence gene/spacer junctions, as well as the entire internal transcribed spacer regions in the cases of yeast, Xenopus, rat, and mouse (3-7). In yeast, the 3' termini of precursor and mature rRNAs share a sequence homology which suggests a recognition signal for processing; the same is true for the 5' terminus/spacer junctions (3,4). However, equivalent features are not apparent in Xenopus, rat, or mouse rDNA (5-7). Comparison of mature RNA-internal transcribed spacer junctions among species revealed no consensus sequences outside of the conserved termini of the rRNA (viz., 3 '-18S, 5'-5.8S, and 5'-26/28S), although some homologies were found between Xenopus and rat spacer regions (6).In this paper, we present the nucleotide sequence of a portion of

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“…Further, this 4b tract was also found in the loop of the gap region of prosoQhi1A 5.8S rRNA (but not in Sciara 5.8S rRNA, where UAUU is a relevant sequence (19)). Such a tract was not observed at all in the corresponding region of other 5.8S rRNAs so far studied which do not undergo the cleavage reaction (20). Not only presence of common structures in the hidden break region of insect 28S rRNA and in dipteran 5.8S rRNA, but also absence of these structures in 28S species without the hidden break and common 5.8S molecules, may be taken to indicate that specific signals essential to the processing events have been generally conserved.…”

Section: Discussionmentioning

confidence: 79%

Molecular mechanism of introduction of the hidden break into the 28S rRNA of insects: implication based on structural studies

Fujiwara

Ishikawa

1986

Nucl Acids Res

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We determined the structure around the gap region between 28S alpha and 28S beta rRNA of Bombyx mori, a lepidopteran insect, to know the introduction mechanism of the hidden break, an interruption of the phosphodiester bond specific to the 28S rRNA of protostomes. Sequence analysis and S1 nuclease mapping suggested that a stretch of 30 nucleotides is excised from the mid region of the 28S rRNA to generate the hidden break. The length of excluded stretch was very various among three insects so far studied. However, the gap responsible for the hidden break was located in a fixed position in the 28S rRNA irrespective of insect species. It was suggested that extremely AU-rich sequence including the specific UAAU tract forming a loop can be a signal for the introduction of the hidden break. The same signal seemed also involved in splitting the dipteran 5.8S rRNA.

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Primary and secondary structures ofTetrahymenaand aphid 5.8S rRNAs: Structural features of 5.8S rRNA which interacts with the 28S rRNA containing the hidden break

Cited by 20 publications

References 28 publications

A universal model for the secondary structure of S.8S ribosomal RNA molecules, their contact sites with 28S ribosomal RNAs, and their prokaryotic equivalent

A universal model for the secondary structure of S.8S ribosomal RNA molecules, their contact sites with 28S ribosomal RNAs, and their prokaryotic equivalent

Nucleotide sequence of the 18S–26S rRNA intergene region of the sea urchin

Molecular mechanism of introduction of the hidden break into the 28S rRNA of insects: implication based on structural studies

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