Primary and secondary structure of a new viroid ‘species’ (CbVd 1) present in theColeus blumeicultivar ‘Bienvenue’

Nucleotide sequence of the original Brazilian isolate of coleus yellow viroid from Solenostemon scutellarioides and infectivity of its complementary DNA

“…The nt differences induced only local structural changes at the sites where they occurred, without causing significant alterations in the overall secondary structure of the CYVd molecule when compared with CbVd 1. Our data support the current classification of viroids and the notion that the viroid-like molecule from S. scutellarioides is the prototype of a new subgroup (Spiker et al, 1990). The present classification system is also supported by a consensus phylogenetic tree obtained for some representative members of viroids and viroid-like satellite RNAs (Elena et al, 1991 ;Hernandez & Flores, 1992).…”

“…Although resembling a viroid in size and molecular structure, no conclusive evidence of the viroid nature of CbVd 1 was obtained because Koch's postulates were apparently not completed (Spiker et al, 1990). Even though not fully characterized as a viroid, CbVd 1 was still classified as the prototype of a new class of viroids (Spiker et al, 1990). In this present study, we report the complete nucleotide sequence of the original CYVd from S. scutellarioides and its relationship to CbVd 1.…”

“…Transmission tests using purified CYVd ssRNA were reported later (Fonseca et al, 1990). A similar viroid-like molecule, tentatively named Coleus blumei viroid 1 (CbVd 1), was isolated shortly after from S. scutellarioides' Bienvenue' in Germany and sequenced (Spiker et al, 1990). Although resembling a viroid in size and molecular structure, no conclusive evidence of the viroid nature of CbVd 1 was obtained because Koch's postulates were apparently not completed (Spiker et al, 1990).…”

“…A similar viroid-like molecule, tentatively named Coleus blumei viroid 1 (CbVd 1), was isolated shortly after from S. scutellarioides' Bienvenue' in Germany and sequenced (Spiker et al, 1990). Although resembling a viroid in size and molecular structure, no conclusive evidence of the viroid nature of CbVd 1 was obtained because Koch's postulates were apparently not completed (Spiker et al, 1990). Even though not fully characterized as a viroid, CbVd 1 was still classified as the prototype of a new class of viroids (Spiker et al, 1990).…”

A viroid from Nematanthus wettsteinii plants closely related to the Columnea latent viroid

“…1, lane 8). Plant extracts infected with different viroids of sizes ranging from 248 nt [Coleus blumei viroid (CbVd 1); Spieker et al, 1990] to 376 nt [citrus exocortis viroid (CEVd); Gross et al, 1982] were included in the survey experiments for comparison (Fig. 1, lanes 2 to 8).…”