Primary analysis and screening of microRNAs in gastric cancer side population cells

Zhang, Haihong; Gu, Guo-Li; Zhang, Xiangyang; Li, Fengzhi; Ding, Li; Fan, Qin; Wu, Rongqian; Shi, Wei; Wang, Xinyan; Chen, Lin; Wei, Xueming; Yuan, Xiao-Ying

doi:10.3748/wjg.v21.i12.3519

Cited by 13 publications

(11 citation statements)

References 32 publications

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“…MiR‐324‐5p was increased in the side population (SP) cells of the gastric cancer cell line MKN‐45 and has been suggested to play an important role in maintaining and regulating the characteristics of SP cells . However, its specific function and mechanism in gastric cancer are not completely understood.…”

Section: Introductionmentioning

confidence: 99%

“…MiR-324-5p was increased in the side population (SP) cells of the gastric cancer cell line MKN-45 and has been suggested to play an important role in maintaining and regulating the characteristics of SP cells. [10] However, its specific function and mechanism in gastric cancer are not completely understood. MiRNAs existed in RNA-induced silencing complex (RISC) could inhibit the translation and induce degradation of the target mRNAs through interacting with mRNA 3 0 -untranslated regions (3 0 -UTR).…”

Section: Introductionmentioning

confidence: 99%

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MiR-324-5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8

Lin

Zhou

Zhang

et al. 2018

Journal of Pharmacy and Pharmacology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR-324-5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8

Lin

Zhou

Zhang

et al. 2018

Journal of Pharmacy and Pharmacology

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“…Previous study reported that MiR‐3175 could promote cell proliferation, invasion and inhibit apoptosis via regulating its target gene HOXB1 in glioma . However, the role of MiR‐3175 is still controversial in carcinogenesis and pathogenesis; for instance, MiR‐3175 was also found to be upregulated in prostate cancer as an oncogenic miRNA, but downregulated in gastric cancer as a tumor suppressor. To the best of our knowledge, in this study we demonstrated for the first time that MiR‐3175 could promote proliferation, migration, invasion, and EMT of cultured human conjunctival epithelial cells by inhibition of Smad7 directly.…”

Section: Discussionmentioning

confidence: 99%

MiR‐3175 promotes epithelial‐mesenchymal transition by targeting Smad7 in human conjunctiva and pterygium

Zhong

Tang

et al. 2019

FEBS Letters

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Edited by Tamas DalmayMicroRNAs (miRNAs) are involved in the post-transcriptional regulation of mRNAs. However, the function of miRNAs is unclear in epithelial-mesenchymal transition (EMT) of pterygium, a disease characterized by abnormal fibrovascular proliferation and invasion on the ocular surface. Since MiR-3175 is upregulated in pterygium tissues compared to normal conjunctival tissues, in this study, we investigated the role of MiR-3175 in EMT of a cultured human conjunctival epithelium cell line (CCL) and further validated the findings in pterygium tissues from patients. Our study demonstrates that MiR-3175 promotes proliferation, migration, invasion, and EMT of CCL cells by directly inhibiting Smad7. In conclusion, our data suggested that the MiR-3175-Smad7 axis may play a critical role in pathogenesis and development of pterygium.Pathogenesis and development of pterygium X. Zhong et al.

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“…Using a computer-assisted bioinformatics analysis, we predicted that miR-3175 directly targets and suppresses HOXB1 expression. A recent study found that miR-3175 acts as a tumor suppressor in gastric cancer [ 33 ], and microarray profiling demonstrated its association with chronic congestive heart failure [ 34 ]. However, little is known about the effects of miR-3175 on other kinds of human cancer, including glioma.…”

Section: Discussionmentioning

confidence: 99%

HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma

Han

Liu

et al. 2015

PLoS ONE

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The HOXB1 gene plays a critical role as an oncogene in diverse tumors. However, the functional role of HOXB1 and the mechanism regulating HOXB1 expression in glioma are not fully understood. A preliminary bioinformatics analysis showed that HOXB1 is ectopically expressed in glioma, and that HOXB1 is a possible target of miR-3175. In this study, we investigated the function of HOXB1 and the relationship between HOXB1 and miR-3175 in glioma. We show that HOXB1 expression is significantly downregulated in glioma tissues and cell lines, and that its expression may be closely associated with the degree of malignancy. Reduced HOXB1 expression promoted the proliferation and invasion of glioma cells, and inhibited their apoptosis in vitro, and the downregulation of HOXB1 was also associated with worse survival in glioma patients. More importantly, HOXB1 was shown experimentally to be a direct target of miR-3175 in this study. The downregulated expression of miR-3175 inhibited cell proliferation and invasion, and promoted apoptosis in glioma. The oncogenicity induced by low HOXB1 expression was prevented by an miR-3175 inhibitor in glioma cells. Our results suggest that HOXB1 functions as a tumor suppressor, regulated by miR-3175 in glioma. These results clarify the pathogenesis of glioma and offer a potential target for its treatment.

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Primary analysis and screening of microRNAs in gastric cancer side population cells

Abstract: There are key miRNAs expressed within the SP cells of the gastric cancer cell line MKN-45, and include hsa-miR-3175, hsa-miR-203, hsa-miR-130a, hsa-miR-324-5p, hsa-miR-34a, and hsa-miR-25-star.

Cited by 13 publications

References 32 publications

MiR-324-5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8

MiR-324-5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8

MiR‐3175 promotes epithelial‐mesenchymal transition by targeting Smad7 in human conjunctiva and pterygium

HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma

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