Primary Amino Acid and DNA Sequences of Gassericin T, a Lactacin F-Family Bacteriocin Produced by<i>Lactobacillus gasseri</i>SBT2055

Kawai, Yasushi; Saitoh, Boku; Takahashi, Osamu; Kitazawa, Haruki; Saito, Tadao; Nakajima, Hadjime; Itoh, Takatoshi

doi:10.1271/bbb.64.2201

Cited by 63 publications

(46 citation statements)

References 35 publications

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“…The extreme Nterminal portion of E-760 more closely resembled lactobin A (16) and amylovorin L (9) isolated from Lactobacillus amylovorus. Another similar bacteriocin, gassericin T, which is produced by Lactobacillus gasseri (31), contained distinctly identical amino acid sequences in the peptides including the GG VGGA and AVCG motifs. Gassericin T was most similar to a bacteriocin, lactacin F, also produced by various Lactobacillus species (2,37).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Purification of Enterocin E-760 with Broad Antimicrobial Activity against Gram-Positive and Gram-Negative Bacteria

Line

Svetoch

Eruslanov

et al. 2008

Antimicrob Agents Chemother

190

121

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Strain NRRL B-30745, isolated from chicken ceca and identified as Enterococcus durans, Enterococcus faecium, or Enterococcus hirae, was initially identified as antagonistic to Campylobacter jejuni. The isolate produced a 5,362-Da bacteriocin (enterocin) that inhibits the growth of Salmonella enterica serovar Enteritidis, S. enterica serovar Choleraesuis, S. enterica serovar Typhimurium, S. enterica serovar Gallinarum, Escherichia coli O157:H7, Yersinia enterocolitica, Citrobacter freundii, Klebsiella pneumoniae, Shigella dysenteriae, Pseudomonas aeruginosa, Proteus mirabilis, Morganella morganii, Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, Campylobacter jejuni, and 20 other Campylobacter species isolates. The enterocin, E-760, was isolated and purified by cation-exchange and hydrophobic-interaction chromatographies. The proteinaceous nature of purified enterocin E-760 was demonstrated upon treatment with various proteolytic enzymes. Specifically, the antimicrobial peptide was found to be sensitive to beta-chymotrypsin, proteinase K, and papain, while it was resistant to lysozyme and lipase. The enterocin demonstrated thermostability by retaining activity after 5 min at 100°C and was stable at pH values between 5.0 and 8.7. However, activity was lost below pH 3.0 and above pH 9.5. Administration of enterocin E-760-treated feed significantly (P < 0.05) reduced the colonization of young broiler chicks experimentally challenged and colonized with two strains of C. jejuni by more than 8 log 10 CFU. Enterocin E-760 also significantly (P < 0.05) reduced the colonization of naturally acquired Campylobacter species in market age broiler chickens when administered in treated feed 4 days prior to analysis.Microorganisms produce a variety of compounds which demonstrate antibacterial properties. One group of these compounds, the bacteriocins, consists of relatively small bactericidal peptides. The widespread occurrence of bacteriocins in bacterial species isolated from complex microbial communities, such as the intestinal tract, oral surfaces, or other epithelial surfaces, suggests that they may have a regulatory role in terms of population dynamics within bacterial ecosystems. Bacteriocins are defined as compounds produced by bacteria that have a biologically active protein moiety and bactericidal action (51). In recent years, a renewed interest in bacteriocinlike activities has led to the discovery, isolation, and purification of bacteriocins from both gram-negative and gram-positive organisms (28). They are now being considered for a variety of antimicrobial uses in foods and medicine (17, 41).Lactic acid bacteria (LAB) are among the most well known and investigated producers of microbial antagonists. These include the well-characterized bacteriocins (6, 30, 55), potential bacteriocinlike substances (57), and other antagonists not necessarily related to bacteriocins (24,36,44). The LAB are gram-positive, non-spore-forming, catalase-negative organisms devoid of cytochromes. They are anaerobic...

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Section: Discussionmentioning

confidence: 99%

Isolation and Purification of Enterocin E-760 with Broad Antimicrobial Activity against Gram-Positive and Gram-Negative Bacteria

Line

Svetoch

Eruslanov

et al. 2008

Antimicrob Agents Chemother

190

121

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“…Bacteriocin production has been shown for a number of such strains (Kawai et al 2000), and is often proposed as a beneficial characteristic of probiotic bacteria (Klaenhammer and Kullen 1999;Fooks and Gibson 2002). Lately intense investigation on bacteriocins from LAB, has led to considerable progress toward a better understanding of these substances.…”

Section: Introductionmentioning

confidence: 99%

Production and physicochemical characterization of acidocin D20079, a bacteriocin produced by Lactobacillus acidophilus DSM 20079

Deraz

Hedström

Karlsson

et al. 2006

World J Microbiol Biotechnol

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Lactobacillus acidophilus DSM 20079 is the producer of a novel bacteriocin termed acidocin D20079. In this paper, a partial sequence of this peptide is determined, together with data on its secondary structure. A modification of the MRS-growth medium (replacing the detergent Tween 80 with oleic acid), was shown to improve the production level of the peptide by one order of magnitude, as well as to stabilize the activity level. Addition of a detergent (Tween 20, less interfering in mass spectrometric analysis), was however necessary for solubilization of the purified acidocin D20079. Digestion of the peptide followed by de-novo sequencing of generated fragments, allowed determination of a partial sequence consisting of 39 of the totally estimated 65 residues. Acidocin D20079 has a high content of glycine residues, hydrophobic residues, and acidic residues. No modified amino acids were found. Edman degradation, and C-terminal sequencing failed, suggesting that the peptide may be cyclic, and a novel member of class IIc bacteriocins.Circular dichroism spectroscopy and secondary structure prediction showed random coil conformation in aqueous solution, but secondary structure was induced in the presence of sodium-dodecyl sulfate. The data could be fitted assuming 2-13% of the residues to be in a-helix and 23-27% of the residues to be in b-strand conformation. This indicates that a membrane/membrane-mimicking hydrocarbon-water interface induces an active conformation.

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“…DNA homology searches showed similarities of acidocin LF221 A to brochocin B (BrcB), lactococcin N (LcnN) and thermophilin B (ThmB) which complemented the BrcA, LcnM and ThmA, respectively, forming the two-component bacteriocins known as brochocin-C (McCormick et al, 1998), lactococcin MN (Van Belkum et al, 1991) and thermophilin 13 (Marciset et al, 1997). Acidocin LF221 B exhibited some homology to lactacin F (Allison et al, 1994) and was virtually identical to gassericin T (Kawai et al, 2000). As lactacin F and gassericin T belong to subgroup B of class II bacteriocins, it seems likely that acidocin LF221 B belongs to this group as well.…”

Section: Bacteriocins Of Lf221 and K7mentioning

confidence: 99%

Lactobacillus gasseri LF221 and K7 — from isolation to application

Rogelj

Matijašić

2006

Biologia

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Abstract:The article presents research findings on two human strains with probiotic activity. On the basis of API 50 CHL fermentation pattern, PCR by species-specific primers and sequencing of the V2-V3 region of 16S rRNA both strains designated as LF221 and K7 were identified as members of the Lactobacillus gasseri species. Two LF221 bacteriocins, acidocin LF221 A and B were purified and sequenced. They were classified as members of the two-component class II bacteriocins. Among basic probiotic properties, the survival under conditions in gastro-intestinal tract, ability to adhere to cultured intestinal enterocytes and pig's mucosa and stimulation of the immune response were demonstrated. In in vivo study of 24 weaned piglets, the survival rate of K7 Rif r and LF221 Rif r was quantified by selective enumeration on MRS agar with rifampicin. The survival of both strains was good (2.9 × 10 5 cfu of K7 Rif r /g faeces; 4.8 × 10 5 cfu of LF221 Rif r /g) and the LF221 Rif r /K7 Rif r viable cells were found either in the mucosa of duodenum, jejunum or in the ileum. The possible effect of K7 to inhibit adhesion of E. coli O8:K88 to enterocytes was studied on Caco-2 cultured cells, on tissue obtained from small intestines of pigs and in vivo on gnotobiotic piglets. Lactobacilli were found to be effective in reducing E. coli adhesion to enterocytes in Caco-2 model, but not on mucosa of pig's jejunum under ex vivo conditions. Competitive exclusion, production of organic acids and stimulation of immune response, were involved in inhibition of E. coli by K7 strain in gnotobiotic piglets. Any inflammatory change in intestines of piglets treated with K7 was observed, which confirmed its safe use. Among the technological parameters the survival and activity of the strains during cheese-making are presented.

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Primary Amino Acid and DNA Sequences of Gassericin T, a Lactacin F-Family Bacteriocin Produced byLactobacillus gasseriSBT2055

Cited by 63 publications

References 35 publications

Isolation and Purification of Enterocin E-760 with Broad Antimicrobial Activity against Gram-Positive and Gram-Negative Bacteria

Isolation and Purification of Enterocin E-760 with Broad Antimicrobial Activity against Gram-Positive and Gram-Negative Bacteria

Production and physicochemical characterization of acidocin D20079, a bacteriocin produced by Lactobacillus acidophilus DSM 20079

Lactobacillus gasseri LF221 and K7 — from isolation to application

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