PRGF-Modified Collagen Membranes for Guided Bone Regeneration: Spectroscopic, Microscopic and Nano-Mechanical Investigations

Rațiu, Cristian; Brocks, Marcel; Costea, T.; Moldovan, Liviu; Cavalu, Simona

doi:10.3390/app9051035

Cited by 26 publications

(29 citation statements)

References 38 publications

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“…In order to obtain accurate results, each nanoindentation test was repeated 16 times, while the optical image of the sample using a microscope allowed precise control between the sample position and the indenter. Young modulus values were obtained from load-displacement curves, by fitting parameters, using the Oliver-Pharr method [31,32].…”

Section: Structural Characterization Of Pva/ha/se-tio 2 Compositesmentioning

confidence: 99%

Novel Hybrid Composites Based on PVA/SeTiO2 Nanoparticles and Natural Hydroxyapatite for Orthopedic Applications: Correlations between Structural, Morphological and Biocompatibility Properties

et al. 2020

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The properties of poly(vinyl alcohol) (PVA)-based composites recommend this material as a good candidate for the replacement of damaged cartilage, subchondral bone, meniscus, humeral joint and other orthopedic applications. The manufacturing process can be manipulated to generate the desired biomechanical properties. However, the main shortcomings of PVA hydrogels are related to poor strength and bioactivity. To overcome this situation, reinforcing elements are added to the PVA matrix. The aim of our work was to develop and characterize a novel composition based on PVA reinforced with Se-doped TiO2 nanoparticles and natural hydroxyapatite (HA), for possible orthopedic applications. The PVA/Se-doped TiO2 composites with and without HA were structurally investigated by FTIR and XRD, in order to confirm the incorporation of the inorganic phase in the polymeric structure, and by SEM and XRF, to evidence the ultrastructural details and dispersion of nanoparticles in the PVA matrix. Both the mechanical and structural properties of the composites demonstrated a synergic reinforcing effect of HA and Se-doped TiO2 nanoparticles. Moreover, the tailorable properties of the composites were proved by the viability and differentiation potential of the bone marrow mesenchymal stem cells (BMMSC) to osteogenic, chondrogenic and adipogenic lineages. The novel hybrid PVA composites show suitable structural, mechanical and biological features to be considered as a promising biomaterial for articular cartilage and subchondral bone repair.

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Section: Structural Characterization Of Pva/ha/se-tio 2 Compositesmentioning

confidence: 99%

Novel Hybrid Composites Based on PVA/SeTiO2 Nanoparticles and Natural Hydroxyapatite for Orthopedic Applications: Correlations between Structural, Morphological and Biocompatibility Properties

et al. 2020

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“…They concluded that the changes in Young modulus values are correlated with the ultrastructural properties of each membrane type, more precisely the porosity; the higher the porosity, the lower the Young modulus value was obtained. After the PRGF treatment, the nanoindentation measurements indicated the Young modulus value ranging in the interval 2.8 -4 GPa, depending on the type of the membrane [8]. One can observe that antibacterial effect depends on the nanoparticles concentration in collagen film, for both bacteria investigated.…”

Section: Resultsmentioning

confidence: 92%

“…In the same time, they demonstrated that Young's modulus of collagen/chitosan films with different ratio was much higher than Young's modulus for collagen films or chitosan films unmodified. Ratiu et al [8] investigated the modifications of resorbable collagen membranes for guided bone regeneration, upon addition of plasma rich in growth factors (PRGF). They concluded that the changes in Young modulus values are correlated with the ultrastructural properties of each membrane type, more precisely the porosity; the higher the porosity, the lower the Young modulus value was obtained.…”

Section: Resultsmentioning

confidence: 99%

“…Collagen-based wound dressings have long been used in the form of sheets, films and membranes, in wound healing and tissue engineering, to cover burn wounds and treat ulcers [3,[5][6][7]. On the other hand, the blend of biopolymers, such as chitosan and collagen, have gained attention for successful and relevant applications as wound dressings due to their potentially beneficial biological properties, such as vehicles for the sustained release of pharmaceuticals, especially growth factors [8] or slow release of encapsulated drugs [9]. Chitosan is a good candidate for encapsulation, being widely used as a drug carrier for many possible routes of administration [10,11], due to his non-toxicity, biocompatibility, biodegradability, and antibacterial activity.…”

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Natural Polymeric Film Encapsulating Propolis Nano-Formulation for Cutaneous Wound Healing

Cavalu¹,

Pasca²,

Brocks³

2019

Mat.Plast.

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The present paper describe the production and characterization of novel collagen films containing propolis encapsulated in chitosan nanoparticles, for biomedical applications such as cutaneous wound healing. Structural and morphological details were investigated by ATR FTIR spectroscopy, SEM and nanoindentation measurements, revealing the collagen fibers aligned in a quasi-parallel distribution, which might be favorable for biomedical applications. Moreover, the vibrational marker bands of propolis were well preserved in the final polymeric mixture, indicating the stability of bioactive compounds upon the encapsulation procedure. The antibacterial effect depends on the nanoparticles concentration in collagen film, the effect being more evident with respect to E. coli than S. aureus. The antioxidant capacity monitored by CUPRAC assay, indicated a synergic effect of chitosan nanoparticles matrix and propolis extract, incorporated in collagen films.

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“…It is well known that platelets play a crucial role in hemostasis and healing processes. Several studies reported that, due to concentrated platelets, the use of autologous plasma rich in growth factors (PRGF) promotes the regeneration of bone and soft tissues, thus improving the therapeutic outcome [ 9 , 10 ]. The activated platelets release a wide variety of biologically active molecules, such as: vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGR), transforming growth factor-beta 1 (TGF- β 1), and insulin-like growth factor-1 (IGF-1), which stimulate the healing process.…”

Section: ⧉ Introductionmentioning

confidence: 99%

Successful management of spontaneous bone regeneration after jaws cystectomy using PRGF approach; case series

Rațiu

Raţiu²,

Cavalu

et al. 2021

Rom J Morphol Embryol

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In the jaws, the healing process of bone defects occurs differently compared with other anatomical sites where healing is done by the formation of scar tissue. This is due to physiological bone remodeling and continuous bone resorption and apposition. In the case of large cysts, there is an increased risk of postoperative infection due to the retraction of the blood clot on the bony walls. The use of plasma rich in growth factors (PRGF), with or without autologous bone addition, for filling the cystic cavities is beneficial not only for its antimicrobial effect, but also due to simultaneously release of growth factors from platelet alpha granules and adhesive proteins. The aim of our work was to emphasize the spontaneous bone healing of the maxilla and mandible after the enucleation of large and medium-sized cysts in asymptomatic patients of different age. The outcome of three patients who underwent surgery for removal of radicular cysts is presented. Imagistic [cone-beam computed tomography (CBCT)] and histological examination was performed aiming to evaluate the quality of new bone at the site of the bone defect, after different time intervals. By using PRGF approach, the healing of mandibular bone occurred 18 months after cystectomy in a smoker patient, 54-year-old, while the healing of maxillary bone using autologous bone mixed with PRGF occurred after six months in a 63-year-old patient. In a young patient (14 years old), the regeneration period was six months, using PRGF and a fibrin cloth for filling the defect.

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PRGF-Modified Collagen Membranes for Guided Bone Regeneration: Spectroscopic, Microscopic and Nano-Mechanical Investigations

Cited by 26 publications

References 38 publications

Novel Hybrid Composites Based on PVA/SeTiO2 Nanoparticles and Natural Hydroxyapatite for Orthopedic Applications: Correlations between Structural, Morphological and Biocompatibility Properties

Novel Hybrid Composites Based on PVA/SeTiO2 Nanoparticles and Natural Hydroxyapatite for Orthopedic Applications: Correlations between Structural, Morphological and Biocompatibility Properties

Natural Polymeric Film Encapsulating Propolis Nano-Formulation for Cutaneous Wound Healing

Successful management of spontaneous bone regeneration after jaws cystectomy using PRGF approach; case series

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