Previous liver regeneration induces fibro-protective mechanisms during thioacetamide-induced chronic liver injury

Gratte, Francis D.; Pasic, Sara; Bakar, N. Dianah B. Abu; Gogoi-Tiwari, Jully; Liu, Xiao; Carlessi, Rodrigo; Kisseleva, Tatiana; Brenner, David A.; Ramm, Grant A.; Olynyk, John K.; Tirnitz–Parker, Janina E.E.

doi:10.1016/j.biocel.2021.105933

Cited by 4 publications

(3 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liver is metabolic structure of the body with high susceptibility to the toxic effects of thioacetamide. Thioacetamid (TAA) has been chemical compound frequently used to induce liver injury in experimental animals, the chronic effects of this chemical lead to liver cirrhosis ( Su et al, 2019 , Abood et al, 2020 , Gratte et al, 2021 ). In existing training, TAA was practiced to induce the liver toxicity to appraise the hepatoprotective influence of methanolic extract of green tea.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats

Shareef

Ibrahim

Alzahrani

et al. 2022

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Ethnopharmacological relevance Since ancient times, herbal medicines have been applied in the treatment of cancer. Tea, derivative from the dried leaves of Camellia sinensis (L.) Kuntze plant is the most popular beverage globally after water and is available in various forms. Green tea has been expansively investigated for its beneficial properties of cancer prevention and therapy. The goal of the research: The current study was conducted to evaluate the hepaprotective character of methanolic green tea extract and its mechanism of action contrary to thioacetamide (TAA)-produced liver fibrosis of Sprague Dawley rats. Materials and Methods Thirty rodents were equally placed in 5 clusters including normal control, TAA group as a positive control, silymarin as standard drug control, and treatment groups consisting of high dose and a low dose Camellia sinensis. Rats in experimental clusters by mouth fed with C. sinensis at 250 mg/kg or 500 mg/kg daily for 2 months. After 60 days, all rats were sacrificed. Blood specimens were gathered for liver biochemical examination. Livers of all groups were dissected out and subjected to histopathological examination through the Hematoxylin and Eosin stain, Masson trichrome, and immunohistochemistry stains (PCNA). Liver tissue homogenate was also analyzed for antioxidant activity parameters. Results Gross morphological examination showed a regular liver architecture in C. sinensis fed collections compared to the TAA sets. Histology of rat’s liver fed with C. sinensis showed an important decrease in the liver index with hepatic cells propagation, mild cellular injury, and immunostaining showed significant down-expression of proliferating cell nuclear antigen (PCNA). TAA produced liver fibrosis through a significant increase in serum alanine transferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin. Total protein and albumin also decreased in the TAA group. Moreover, the reduction of antioxidant enzyme activity including superoxide dismutase and catalase as well as the increase in malondialdehyde was detected in the TAA control group. Meanwhile, an abnormal level of liver biochemical parameters was restored closer to the normal levels in serum of the C. sinensis-fed clusters. In addition, C. sinensis fed assemblies showed elevated antioxidative enzymes activity with a reduction in malondialdehyde level comparable to the levels in silymarin-treated rats. Conclusions Green tea potentially inhibited the progression of liver cirrhosis, down -regulation of PCNA proliferation, prevented oxidation of hepatocytes, recovered SOD and CAT enzymes, condensed MDA and reduced cellular inflammation.

show abstract

Section: Discussionmentioning

confidence: 99%

Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats

Shareef

Ibrahim

Alzahrani

et al. 2022

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…The induction of hepatic damage through TAA injection is one of the experimental methods widely used to identify hepatoprotective agents due to its outstanding solubility in the water, and a prolonged injury and recovery pattern, giving significant time to study mechanisms 19 . TAA‐induced liver cirrhosis is considered to be a typical model because it mimics many aspects of the human disease that lead to the development of fibrosis, cirrhosis and liver cancer in rats 20,21 . Qu is a member of the flavonoid family and more specifically a subclass called flavonol and is widely distributed in the plant kingdom.…”

Section: Discussionmentioning

confidence: 99%

“… 19 TAA‐induced liver cirrhosis is considered to be a typical model because it mimics many aspects of the human disease that lead to the development of fibrosis, cirrhosis and liver cancer in rats. 20 , 21 Qu is a member of the flavonoid family and more specifically a subclass called flavonol and is widely distributed in the plant kingdom. In the mouth, Qu released from the food can interact with salivary protein and form soluble Qu‐protein binary aggregates.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective activity of Lactéol® forte and quercetin dihydrate against thioacetamide‐induced hepatic cirrhosis in male albino rats

Saad,

Oda,

Alexiou

et al. 2024

J Cellular Molecular Medi

View full text Add to dashboard Cite

Liver cirrhosis is a silent disease in humans and is experimentally induced by many drugs and toxins as thioacetamide (TAA) in particular, which is the typical model for experimental induction of hepatic fibrosis. Thus, the objective of the present study was to elucidate the possible protective effects of lactéol® forte (LF) and quercetin dihydrate (QD) against TAA‐induced hepatic damage in male albino rats. Induction of hepatotoxicity was performed by TAA injection (200 mg/kg I/P, twice/ week) in rats. LF (1 × 109 CFU/rat 5 times/week) and QD (50 mg/kg 5 times/week) treated groups were administered concurrently with TAA injection (200 mg/kg I/P, twice/ week). The experimental treatments were conducted for 12 weeks. Hepatotoxicity was evaluated biochemically by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma‐glutamyl transferase (GGT) in the serum and histopathologically with the scoring of histopathological changes besides histochemical assessment of collagen by Masson's trichrome and immunohistochemical analysis for α‐smooth muscle actin (α‐SMA), Ki67 and caspase‐3 expression in liver sections. Our results indicated that LF and QD attenuated some biochemical changes and histochemical markers in TAA‐mediated hepatotoxicity in rats by amelioration of biochemical markers and collagen, α‐SMA, Ki67 and caspase3 Immunoexpression. Additionally, LF and QD supplementation downregulated the proliferative, necrotic, fibroblastic changes, eosinophilic intranuclear inclusions, hyaline globules and Mallory‐like bodies that were detected histopathologically in the TAA group. In conclusion, LF showed better hepatic protection than QD against TAA‐induced hepatotoxicity in rats by inhibiting inflammatory reactions with the improvement of some serum hepatic transaminases, histopathological picture and immunohistochemical markers.

show abstract

Molecular mechanisms in thioacetamide-induced acute and chronic liver injury models

Ezhilarasan

2023

Environmental Toxicology and Pharmacology

View full text Add to dashboard Cite

Previous liver regeneration induces fibro-protective mechanisms during thioacetamide-induced chronic liver injury

Cited by 4 publications

References 36 publications

Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats

Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats

Hepatoprotective activity of Lactéol® forte and quercetin dihydrate against thioacetamide‐induced hepatic cirrhosis in male albino rats

Molecular mechanisms in thioacetamide-induced acute and chronic liver injury models

Contact Info

Product

Resources

About