Preventive Effects of Unsei-in and Oren-gedoku-to, Chinese Traditional Medicines, Against Rat Paw Oedema and Abdominal Constriction in Mice

Abstract

Oren-gedoku-to and Unsei-in are complex mixtures of ingredients derived from plants. These two drugs are clinically most frequently used in the treatment of Behçet's disease, we have investigated the anti-inflammatory and analgesic actions of Oren-gedoku-to and Unsei-in using a battery of tests; rat paw oedema induced by five different agents; abdominal constriction; mouse ear swelling; and dye leakage tests, designed to clarify the therapeutic potential of these medicines. The findings in this study that these medicines are able to inhibit the rat paw oedema, suppress the abdominal constriction and inhibit the increased dye permeability confirm the analgesic and anti-inflammatory effects of these compounds. From these results there seems a clear rationale for exploring the effectiveness of these Chinese medicines in the treatment of chronic and acute inflammatory diseases.

Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in Mice

“…In the present experiments, we reconfirmed the effectiveness of repeated administration of Unsei-in at a dose of 300 mg / kg and found that the lower dose of 100 mg / kg was without effect. The effective dose is similar to those in other experiments using mice, such as carrageenan-induced edema (10) and acetic acidinduced abnormal constriction (11). The clinical dose of Unsei-in is 2,400 mg per day.…”

Analgesic and anti-inflammatory effects ofMangifera indica L. extract (Vimang)

“…Moreover, we have used the carrageenan-induced paw oedema as an in vivo model of inflammation (Winter et al, 1963) because it is a screening procedure in which the involvement of the cyclooxygenase products of arachidonic acid metabolism and the production of reactive oxygen species are well established (Smith et al, 1974). It is reported that the carrageenan oedema shows three distinct phases, namely an initial release of histamine and 5-hydroxytryptamine, a second phase mediated by kinins and a third phase (about 5 h of oedema) in which the mediator is suspected to be prostaglandin (Wang and Mineshita, 1996). Vimang, dependently of dose, inhibited the carrageenan paw oedema in a similar manner to indomethacin in rats at 5 h. Meanwhile, at 3 h the effect of Vimang on hind paw oedema in the guinea-pigs was comparable to naproxen.…”

An Herbal Medicine Orengedokuto Prevents Indomethacin-Induced Enteropathy

“…47) OGT influences microcirculatory blood flow, which is one of the mechanisms responsible for the efficacy of OGT. 48) Finally, there are many reports that OGT has anti-inflammatory effects, [49][50][51][52] such as the ability to inhibit rat paw oedema. Efforts to identify the active ingredients of OGT and the properties of each ingredient are now under way.…”