Preventive effects of rutin on lysosomal enzymes in isoproterenol induced cardio toxic rats: Biochemical, histological and in vitro evidences

Prince, P. Stanely Mainzen; Priya, S

doi:10.1016/j.ejphar.2010.08.054

Cited by 24 publications

(10 citation statements)

References 36 publications

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“…We observed significantly increased infarct size and an absence of the normal red colour/bright spot in ISO-induced rats. The metabolites of ISO were involved in free radical production and damaged the cardiomyocytes [24]. Oral pretreatment with ellagic acid significantly reduced the infarct size in ISOinduced rats.…”

Section: Discussionmentioning

confidence: 99%

Mechanistic Clues in the Protective Effect of Ellagic Acid Against Apoptosis and Decreased Mitochondrial Respiratory Enzyme Activities in Myocardial Infarcted Rats

Maharajan

Quine²

2011

Cardiovasc Toxicol

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Our previous study described the cardioprotective effects of ellagic acid in an isoproterenol-induced myocardial infarction model. In this study, we are reporting the mechanism of protective action of ellagic acid with respect to apoptosis and mitochondrial respiratory enzymes. Ellagic acid (7.5 and 15 mg/kg) was administered orally as a pretreatment for 10 days. Then, isoproterenol (100 mg/kg) was injected subcutaneously to rats at an interval of 24 h for 2 days. Myocardial infarction was quantified by planimetry. Apoptosis was measured by apoptotic gene expressions. The levels of mitochondrial respiratory enzymes were also measured. Isoproterenol-induced myocardial infarcted rats showed increased infarct size, a decrease in myocardial expression of the Bcl-2 gene and an increase in myocardial expression of the BAX gene. Fas ligand and caspases were markedly elevated along with compromised respiratory marker enzymes in isoproterenol-induced rats. Ellagic acid pretreatment reduced the infarct size, regulated apoptotic gene expressions and enhanced the activities of mitochondrial respiratory marker enzymes and cell viability, thereby protecting the myocardium against isoproterenol-induced myocardial infarction. The decreased infarct size associated with inhibited apoptosis and increased respiratory marker enzymes provide insight on the role of ellagic acid in antiapoptotic mechanism, and it may be the reason for its cardioprotective activity.

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Section: Discussionmentioning

confidence: 99%

Mechanistic Clues in the Protective Effect of Ellagic Acid Against Apoptosis and Decreased Mitochondrial Respiratory Enzyme Activities in Myocardial Infarcted Rats

Maharajan

Quine²

2011

Cardiovasc Toxicol

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“…73 It has been reported that rutin has several pharmacological actions, including anti-oxidant, cytoprotective, antiplatelet, antithrombic, vasoprotective and cardioprotective. 74 For example, rutin was found to scavenge free radicals, stabilize membranes and protect lysosomal membranes against isoproterenol-induced cardiac damage. 74 In the case of ischaemia-reperfusion injury, rutin produced better cardioprotection than quercetin in normal and diabetic rats, an effect that was mediated by regulation of NO production.…”

Section: Rutinmentioning

confidence: 99%

“…74 For example, rutin was found to scavenge free radicals, stabilize membranes and protect lysosomal membranes against isoproterenol-induced cardiac damage. 74 In the case of ischaemia-reperfusion injury, rutin produced better cardioprotection than quercetin in normal and diabetic rats, an effect that was mediated by regulation of NO production. 75 In addition, rutin exerts its anti-apoptotic effects in H9c2 cells via regulation of the Bcl-2 ⁄ Bax ratio, as well as endoplasmic reticulum stress proteins.…”

Section: Rutinmentioning

confidence: 99%

Active chemical compounds of traditional Chinese medicine Herba Leonuri: implications for cardiovascular diseases

Liu

Pan

Zhu

2012

Clin Exp Pharma Physio

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1. Herba Leonuri, a traditional Chinese medicine, has a long history of development and application in China. In particular, Herba Leonuri has been widely used in the treatment of gynaecological and obstetric disorders for thousands of years. Recent studies have shown that Herba Leonuri has many different effects. 2. Herba Leonuri contains several active components, including alkaloids, flavonoids, diterpenes compounds and fatty acids. Recent studies have shown that these molecules exert beneficial effects in coronary artery disease and cerebral ischaemia. Thus, these molecules may become novel candidates for drug discovery and development. 3. The present review provides an overview of the chemical composition, with a focus on biological activity, of Herba Leonuri to stimulate new studies on traditional Chinese medicine.

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“…Rutin [3,[19][20][21][22][23] Anti-hepatotoxic, anti-oxidant, cAMP-phosphodiesterase-inhibitor, vasopressor, vasodilator, anti-inflammatory, cytoprotective 5 Narcissin [24][25][26][27] Anti-inflammatory, anti-oxidant, hepatoprotective 4…”

Section: Analyte [References] Reported Activity Herb Mars Ranking Abmentioning

confidence: 99%

The Quality Assessment of Commercial Lycium Berries Using LC-ESI-MS/MS and Chemometrics

Jarouche

Suresh

Hennell

et al. 2019

Plants

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Lycium (also known as Goji berry) is used in traditional Chinese medicine (TCM) with claimed benefits, including eye and liver protection, immune system fortification and blood glucose control. The commercially available product comes from either the L. barbarum or L. chinense species, with the former dominating the marketplace due to its better taste profile. The main objective of this study was to develop a validated LC-ESI-MS/MS method to quantify multiple key bio-active analytes in commercially available Lycium berries and to qualitatively assess these samples using a principal component analysis (PCA). A LC-ESI-MS/MS method for the quantitation of seven analytes selected using the Herbal Chemical Marker Ranking System (Herb MaRS) was developed. The Herb MaRS ranking system considered bioavailability, bioactivity and physiological action of each target analyte, its intended use and the commercial availability of an analytical standard. After method optimization combining high resolving power with selective detection, seven analytes were quantified and the Lycium samples were quantitatively profiled. Chromatographic spectra were also obtained using longer run-time LC-UV and GC-MS methods in order to qualitatively assess the samples using a principal component analysis (PCA). The result of the method validation procedure was a 15.5 min LC-ESI-MS/MS method developed for the quantification of seven analytes in commercial Lycium samples. Wide variation in analyte concentration was observed with the following results (analyte range in mg/g): rutin, 16.1-49.2; narcissin, 0.37-1.65; nictoflorin, 0.26-0.78; coumaric acid, 6.84-12.2; scopoletin, 0.33-2.61; caffeic acid, 0.08-0.32; chlorogenic acid, 1.1-9.12. The quantitative results for the L. barbarum and L. chinense species samples indicate that they cannot be differentiated based on the bio-actives tested. A qualitative assessment using PCA generated from un-targeted LC-UV and GC-MS phytochemical spectra led to the same conclusion. The un-targeted quantitative and qualitative phytochemical profiling indicates that commercial L. barbarum and L. chinense cannot be distinguished using chemical analytical methods. Genetic fingerprinting and pharmacological testing may be needed to ensure the efficacy of commercial Lycium in order to validate label claims.

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Preventive effects of rutin on lysosomal enzymes in isoproterenol induced cardio toxic rats: Biochemical, histological and in vitro evidences

Cited by 24 publications

References 36 publications

Mechanistic Clues in the Protective Effect of Ellagic Acid Against Apoptosis and Decreased Mitochondrial Respiratory Enzyme Activities in Myocardial Infarcted Rats

Mechanistic Clues in the Protective Effect of Ellagic Acid Against Apoptosis and Decreased Mitochondrial Respiratory Enzyme Activities in Myocardial Infarcted Rats

Active chemical compounds of traditional Chinese medicine Herba Leonuri: implications for cardiovascular diseases

The Quality Assessment of Commercial Lycium Berries Using LC-ESI-MS/MS and Chemometrics

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