Preventive Effects of Phoenix Dactylifera Polyphenols Against 7,12-Dimethylbenz(a)anthracene-Induced Mammary Cancer

Abstract

Objectives: Polyphenols are vital micronutrients, in our diet, which have a role in the prevention of progressive cancer and cardiovascular diseases developing. The main objective of this research was to evaluate the hemopreventive effects of some polyphenols of Phoenix dactylifera pits on 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary cancer of female albino rats.Methods: The phenolics of P. dactylifera pits (Zahidi cultivar) were prepared by successive steps; extraction by ethanol:methanol:HCl:H2O, adsorption chromatography using a silica gel column and preparative high performers chromatography. The cytotoxic activity of the phenolics was detected against human breast cancer cell line (MCF-7). The acetone phenolic fraction, 50 female albino rats, and DMBA carcinogen were used to study the preventive effects.Results: The acetone phenolic fraction contained considerable amounts of the total phenolics and having antioxidant activity, which inhibit cancer cell line MCF-7 growth, and the inhibitory concentration 50% of cells (IC50) at 24 hrs was 202 μg/ml for MCF-7 and 480 μg/ml for WRL-68. The purified phenolic compounds may be related to genistein and quercetin derivatives that inhibited MCF-7 cell line growth and IC50 were 1030 and 203.9 μg/ml, respectively, compared with negligible effects on normal cell line. The acetone phenolic fraction prevented mammary cancer formation in the DMBA-induced rat model.Conclusions: The phenolics of date palm had chemopreventive effects against DMBA-induced mammary cancer, and they required further research to clarify the possible mechanisms that might have contributed to the preventive effects against mammary cancer.

Date fruit as a promising source of functional carbohydrates and bioactive compounds: A review on its nutraceutical potential

“…They also inhibit cancer cell line MCF-7 growth without having adverse effects on normal cell line. Besides, the acetone phenolic fraction prevented mammary cancer formation in the 7,12-dimethylbenzene(a)anthracene (DMBA) induced mammary cancer in female albino rat model (Omran et al, 2017). The anticancer or antibacterial potential of different nano particle prepared from date extract are elaborated in Table 1.…”

UJI SITOTOKSIK EKSTRAK ETANOL DAN FRAKSI BIJI KURMA AJWA (Phoenix dactylifera L.) TERHADAP SEL KANKER PAYUDARA MCF-7

“…Kerusakan ini dapat menyebabkan terjadinya disregulasi siklus sel (Yasin et al, 2015;Zhang et al, 2013). Omran et al (2017) melaporkan bahwa fraksi aseton biji kurma Zahidi dapat menghambat pertumbuhan sel kanker MCF-7 dengan mencegah pembentukan kanker payudara dengan nilai IC50 sebesar 202 𝜇g/mL. Senyawa fenolik yang mungkin terkait dalam penghambatan pertumbuhan sel kanker MCF-7 adalah quersetin dan genistein.…”

“…MCF-7. Hasil penelitian ini tidak sejalan dengan penelitian sebelumnya yang dilakukan menggunakan kultivar kurma yang berbeda yaitu biji kurma Zahidi yang menunjukkan nilai IC50 sebesar 202 µg/ mL yang termasuk dalam kategori aktivitas sitotoksik lemah(Omran et al, 2017). Diduga adanyaAl-Zubaidy, A.…”