Preventive Effect of Two New Neurotensin Analogues on Parkinson’s Disease Rat Model

Lazarova, Maria; Popatanasov, Аndrey; Klissurov, R.; Stoeva, Stanila; Pajpanova, Tamara; Kalfin, Reni; Tancheva, Lyubka

doi:10.1007/s12031-018-1171-6

Cited by 15 publications

(11 citation statements)

References 45 publications

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“…In the present study we confirmed the relation between neuronal degeneration in 6-OHDA model of PD and OS as a main pathogenic factor in disease progression [4,6,105]. Oxidation of 6-OHDA by molecular oxygen or monoamine oxidase underlies its neurotoxicity in the brain and leads to production of intracellular H 2 O 2 which can be transformed into highly reactive hydroxyl radicals, a reduction in glutathione (GSH) and SOD activity, an increase in LPO and production of superoxide free radicals causing cell damage [106].…”

Section: Discussionsupporting

confidence: 85%

Neuroprotective Mechanisms of Three Natural Antioxidants on a Rat Model of Parkinson’s Disease: A Comparative Study

et al. 2020

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We compared the neuroprotective action of three natural bio-antioxidants (AOs): ellagic acid (EA), α-lipoic acid (LA), and myrtenal (Myrt) in an experimental model of Parkinson’s disease (PD) that was induced in male Wistar rats through an intrastriatal injection of 6-hydroxydopamine (6-OHDA). The animals were divided into five groups: the sham-operated (SO) control group; striatal 6-OHDA-lesioned control group; and three groups of 6-OHDA-lesioned rats pre-treated for five days with EA, LA, and Myrt (50 mg/kg; intraperitoneally- i.p.), respectively. On the 2nd and the 3rd week post lesion, the animals were subjected to several behavioral tests: apomorphine-induced rotation; rotarod; and the passive avoidance test. Biochemical evaluation included assessment of main oxidative stress parameters as well as dopamine (DA) levels in brain homogenates. The results showed that all three test compounds improved learning and memory performance as well as neuromuscular coordination. Biochemical assays showed that all three compounds substantially decreased lipid peroxidation (LPO) levels, and restored catalase (CAT) activity and DA levels that were impaired by the challenge with 6-OHDA. Based on these results, we can conclude that the studied AOs demonstrate properties that are consistent with significant antiparkinsonian effects. The most powerful neuroprotective effect was observed with Myrt, and this work represents the first demonstration of its anti-Parkinsonian impact.

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Section: Discussionsupporting

confidence: 85%

Neuroprotective Mechanisms of Three Natural Antioxidants on a Rat Model of Parkinson’s Disease: A Comparative Study

et al. 2020

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“…Rotation test is the most commonly used method for assessing functional efficacy of the PD animal model. In this study, rotation test was performed according to a previous study [ 12 ]. In brief, the rats in different groups were injected with apomorphine (the dopamine agonist) 2 mg/kg, i.p., and the number of contralateral rotations (turn unilaterally 360 degrees) was counted for a period of 30 min.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Compound Formula Rehmannia against Neurotoxicity and Apoptosis Induced by α‐Syn in In Vivo and In Vitro Models of Parkinson’s Disease

Teng

Yang

Jin

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The present study aimed to investigate the protective effect of compound formula Rehmannia (CFR) against the development of Parkinson’s disease (PD). After the in vivo and in vitro models of PD were established with overexpression α-syn induced, CFR was administrated into the PD model rats for 6 weeks or SK-N-SH cells with coincubation for 48 h. Apomorphine-induced rotation test, CCK8 assay, TUNEL assay, immunofluorescence staining, and western blot assay were performed to evaluate the behavioral changes, cell viability, cell apoptosis, α-syn, GSK-3β, P-GSK-3β (Ser9), P-GSK-3β (Tyr216), and β-catenin expression in PD rats or SK-N-SH cells. PD rat behavior results showed that the rotation numbers were significantly decreased in the CFR treatment group comparing with the AAV-α-syn PD model group. The cell viability suppressed by H2O2 and α-syn in SK-N-SH model cells was also significantly improved with CFR administration. Cell apoptosis and α-syn overexpression observed in PD rats and SK-N-SH cells were also inhibited by CFR treatment. Furthermore, the protein expression of α-syn, GSK-3β, P-GSK-3β (Ser9), P-GSK-3β (Tyr216), and β-catenin in in vivo and in vitro was also significantly regulated by CFR. The present study suggested that CFR may be considered as a potential neuroprotective agent against PD, and this application will require further investigation.

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“…The crude peptides were precipitated into cold diethyl ether. Then, the precipitate was dissolved in 10% CH 3 COOH and desalted by gel filtration on a Sephadex G25.…”

Section: Proceedings Of the 35th European Peptide Symposium Patrick Bmentioning

confidence: 99%

“…Previously, we replaced the terminal arginine units with its non-proteinogenic analogue canavanine (Cav) [1]. These modification resulted in the discovery of enzymatically and hydrolytic (in different values of pH, available physiologically) stable and more potent analogues [2,3].…”

Section: Introductionmentioning

confidence: 99%

Influence of arginine mimetics on the biological effects of NT(8-13) analogues

Michailova¹,

Georgieva²,

Bruckdorfer³

et al. 2018

Proceedings of the 35th European Peptide Symposium

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Preventive Effect of Two New Neurotensin Analogues on Parkinson’s Disease Rat Model

Cited by 15 publications

References 45 publications

Neuroprotective Mechanisms of Three Natural Antioxidants on a Rat Model of Parkinson’s Disease: A Comparative Study

Neuroprotective Mechanisms of Three Natural Antioxidants on a Rat Model of Parkinson’s Disease: A Comparative Study

Protective Effect of Compound Formula Rehmannia against Neurotoxicity and Apoptosis Induced by α‐Syn in In Vivo and In Vitro Models of Parkinson’s Disease

Influence of arginine mimetics on the biological effects of NT(8-13) analogues

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