Preventive effect of pectic oligosaccharides on acute colitis model mice: modulating epithelial barrier, gut microbiota and Treg/Th17 balance

Abstract

Pectin as dietary fiber supplements has shown emerging potential in clinical ulcerative colitis (UC) adjuvant therapy. In this study, the preventive and prebiotic effect of enzymatic-degraded pectic oligosaccharides (POS) was...

Stachyose Exerts Anticolitis Efficacy by Re-balancing Treg/Th17 and Activating the Butyrate-Derived PPARγ Signaling Pathway

“…26,27 The findings of our previous studies suggest that various functional foods possess prebiotic properties by modulating the Treg/ Th17 cell balance. 28,29 On the other hand, there has been limited research on the impact of STA on the crucial pathogenic mechanism of Treg/Th17 balance in UC. In the present study, we initially assessed Treg and Th17 activities in DSS-induced colitis model mice treated with STA (Figure 3).…”

Pectic oligosaccharides ameliorate high-fat diet-induced obesity and hepatic steatosis in association with modulating gut microbiota in mice

“…16,45–47 As an emerging prebiotic, POS has been shown to regulate gut microbiota and play a preventive/therapeutic role in various diseases. 26,48–51 Pectic is involved in a wide range of biological effects, including the suppression of obesity, fatty liver disease, microbiologic inflammation, increased gastrointestinal barrier function and immune strengthening activity by regulating gut microbiota. 52 However, Li et al (2021) suggested that in vivo absorption is difficult for macromolecules, 53 implying that the high molecular weight (Mw) and complex structures of natural pectins limit their bioavailability.…”

“…23 In our previous study, we innovatively utilized enzymatic degradation to generate POS with DP3 as the main by-product, which demonstrated anti-inflammatory activity in a mouse model of DSS-induced colitis and modulating gut microbiota. 26 However, the specific role and potential mechanism of POS in the treatment of obesity have not yet been elucidated. Our study demonstrates that enzymatically degraded POS significantly affects HFD-induced obesity in mice.…”

“…25 Moreover, in our previous study, we innovatively obtained pectin trisaccharide (DP3) as the main component using the enzymatic degradation of pectin and demonstrated its effectiveness in alleviating the symptoms of dextran sulfate sodium (DSS)-induced colitis and modulating the immune homeostasis balance in mice. 26 However, its beneficial effects on anti-obesity and related disorders have rarely been studied.…”

Polysaccharides from Paecilomyces hepiali Prevent Acute Colitis in Association with Modulating Gut Microbiota and Treg/Th17 Immune Balance in Mice

“…The DNA of gathered fecal samples were abstracted for PCR amplification and 16S RNA sequencing based on a previous description [ 58 ]. Subsequently, data analysis method of gut microbiota distribution and structure were from our previous study [ 58 ].…”