Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats

Long, Xingyao; Wu, Haibo; Zhou, Yujing; Wan, Yunxiao; Kan, Xuemei; Gong, Jianjun; Zhao, Xinquan

doi:10.3389/fnut.2022.852012

Cited by 26 publications

(8 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The thermocycling conditions were: Pre-denaturation at 95˚C for 5 min; followed by 35 cycles of annealing at 58˚C for 50 sec and extension at 72˚C for 90 sec; and a final extension at 72˚C for 10 min. GAPDH was used as an internal reference, and the expression was calculated using the 2 -∆∆Cq method ( 16 , 17 ). The sequences of the primers used for qPCR are listed in Table I .…”

Section: Methodsmentioning

confidence: 99%

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂

Hu,

Wang,

et al. 2023

Exp Ther Med

View full text Add to dashboard Cite

Peucedanum praeruptorum Dunn extract (PPDE) is a well-known treatment used in traditional Chinese medicines, where it is most commonly used to treat coughs and symptoms such as headaches and fever. In the present study, the antioxidant capacity of PPDE in vitro was determined by scavenging experiments using DPPH, ABTS + ·, ·OH, and ·O 2 - . The cell survival rate was determined by MTT assay. The MDA, SOD, CAT, GSH, and GSH-Px content were determined by colorimetry assays. The expression levels of antioxidant genes SOD, CAT, GSH, and GSH-Px were assessed by reverse transcription-quantitative PCR. HPLC was used to identify the PPDE components. The results suggested that PPDE had scavenging effects on DPPH, ABTS, hydroxyl, and superoxide anion radicals in a concentration-dependent manner; H 2 O 2 treatment resulted in oxidative stress in LLC-PK1 cells, and the degree of injury of LLC-PK1 cells following PPDE treatment was improved, which was positively correlated with its concentration. Peucedanum praeruptorum Dunn extract treatment reduced the content of MDA and increased the content of CAT, SOD1, GSH, and GSH-Px. The mRNA expression levels of antioxidant genes detected by quantitative PCR were consistent with changes in CAT, SOD, GSS, and GSH-Px. Additionally, the trend in CAT, SOD1, GSH, and GSS protein expression levels was also consistent at the mRNA level. PPDE was found to consist of isochlorogenic acid C, myricetin, baicalin, luteolin, and kaempferol. Therefore, PPDE, which was formed of products derived from natural substances, functioned in the inhibition of oxidative damage. The present study aimed to obtain a better understanding of the traditional Chinese medicine Peucedanum praeruptorum Dunn and preliminarily elucidate its antioxidant mechanism at the cellular level. Further animal or human experiments are required to verify the antioxidant effects of PPDE for further development and utilization.

show abstract

Section: Methodsmentioning

confidence: 99%

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂

Hu,

Wang,

et al. 2023

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…The PCR reaction was conducted in a fluorescence PCR instrument (Onestep Plus quantitative PCR instrument, Thermo Fisher Scientific) using a reaction volume of 20 μL for each of the eight tubes. The Ct values of each template were determined using GAPDH as the internal reference gene and the 2 −ΔΔCt method was employed to calculate the relative content of the target genes (13,14).…”

Section: Mrna Expression Intensity Of Mrna In Murine Intestinal Tissuesmentioning

confidence: 99%

Alleviating effect of Lactobacillus pentosus CQZC01 probiotic strain on antibiotic-induced diarrhea in mice

LIU,

CHENG,

LIU

et al. 2024

Acta Poloniae Pharmaceutica - Drug Research

View full text Add to dashboard Cite

This experiment used mice as the experimental subjects to study the alleviating effect of Lactobacillus pentosus CQZC01 (LPCQZC01) on antibiotic-induced diarrhea in mice. After adaptive feeding for seven days, Kunming (KM) mice were randomly divided into the following groups: positive control group, model group, normal group, high concentration group of LPCQZC01 (H-LPCQZC01, 1×109 CFU/mL), and low concentration group of LPCQZC01 (L-LPCQZC01, 1×108 CFU/mL). Except for the normal group, mice were orally administered with lincomycin hydrochloride (120 mg/day) for 7 consecutive days. Changes in body weight, food intake, water consumption, and fecal water content of mice were observed. A histological examination of mouse colon and small intestine sections was done to find out if Lactobacillus pentosus CQZC01 may help mice with antibiotic-induced diarrhea. The findings demonstrated that, in comparison to the model group, mice in the H-LPCQZC01 and L-LPCQZC01 groups had considerably lower blood levels of nterleukin-6 (IL-6), interleukin-17A (IL-17A), 5-hydroxytryptamine (5-HT), and malondialdehyde (MDA), and other variables. In comparison to the model group, the fecal water content in the H-LPCQZC01 and L-LPCQZC01 groups was considerably lower. The H-LPCQZC01 and L-LPCQZC01 groups consumed more food and liquids than the model group did. The H-LPCQZC01 and L-LPCQZC01 groups had more intact colon walls and more compact, regular, and organized intestinal villi. H-LPCQZC01 and L-LPCQZC01 groups had the lower CFTR, EGFR expression and stronger NHE1, NHE2 expression than model group. This study reveals that Lactobacillus pentosus CQZC01 may treat inflammatory lesions in the mouse intestines and successfully treat antibiotic-induced diarrhea in mice.

show abstract

“…Mouse whole blood was obtained through retro-orbital bleeding. The collected samples were centrifuged at 4000 rpm for 10 min at 4 o C to separate the serum [9]. The levels of IL-6, IL-10, IL-1β, and TNF-α in ammatory markers in the serum were measured using ELISA assay kits (Thermo Fisher, MA, USA).…”

Section: Measurement Of In Ammatory Cytokines In Mouse Serummentioning

confidence: 99%

Preventive Effects of Chinese Xinjiang Naturally Fermented Yogurt Separated from Lactobacillus rhamnosus AFY02 on Acute Gouty Arthritis in Mice

Wang,

Fan,

Song

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Introduction This research aims to investigate the mechanism of action of Lactobacillus rhamnosus AFY02 (LR-AFY02) in mice with acute gouty arthritis. Using sodium urate, we created a mouse model of acute gouty arthritis. Methods We then examined the degree of foot swelling, pain threshold, blood biochemical indicators, histopathological changes, and mRNA expression changes to determine the effectiveness and mechanism of LR-AFY02 in preventing acute gouty arthritis in mice. Results The outcomes of the animal experiment demonstrated that LR-AFY02 can decrease the severity of mouse foot edema and raise pain threshold. In the ankle joint tissues of mice with acute arthritis, LR-AFY02 can increase the enzyme activity of superoxide dismutase (SOD) and the level of glutathione (GSH), while lowering the enzyme activity of myeloperoxidase (MPO) and the level of malondialdehyde (MDA). Interleukin-6 (IL-6), IL-10, IL-1β, and tumor necrosis factor-alpha (TNF-α) levels in the blood of mice with acute arthritis are also decreased by LR-AFY02. Histopathological findings demonstrated that LR-AFY02 reduced tissue damage in the mouse foot and ankle joints. The findings of the qPCR experiment showed that LR-AFY02 may suppress the mRNA expression of extracellular signal-regulated kinase 1/2 (ERK1/2), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), IL-6, interferon gamma (IFN-γ), and TNF- in the tissues of the ankle joints of mice with acute arthritis. Additionally, LR-AFY02 has the ability to increase the expression of catalase (CAT), manganese superoxide dismutase (Mn-SOD), and copper/zinc superoxide dismutase (Cu/Zn-SOD). Conclusion As a result, it is clear that Lactobacillus rhamnosus AFY02 is more effective than glucosamine sulfate at preventing acute gouty arthritis. It is a strain with potential as a probiotic.

show abstract

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats

Cited by 26 publications

References 64 publications

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂

Alleviating effect of Lactobacillus pentosus CQZC01 probiotic strain on antibiotic-induced diarrhea in mice

Preventive Effects of Chinese Xinjiang Naturally Fermented Yogurt Separated from Lactobacillus rhamnosus AFY02 on Acute Gouty Arthritis in Mice

Contact Info

Product

Resources

About

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats

Cited by 26 publications

References 64 publications

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H2O2

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H2O2

Alleviating effect of Lactobacillus pentosus CQZC01 probiotic strain on antibiotic-induced diarrhea in mice

Preventive Effects of Chinese Xinjiang Naturally Fermented Yogurt Separated from Lactobacillus rhamnosus AFY02 on Acute Gouty Arthritis in Mice

Contact Info

Product

Resources

About

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂