Preventive and therapeutic effects of Danhong injection on lipopolysaccharide induced acute lung injury in mice

Wan, Limei; Tan, Lei; Wang, Zi-Ran; Liu, Shixin; Wang, Yinling; Liang, Siyu; Zhong, Jianbin; Lin, Han-Sheng

doi:10.1016/j.jep.2013.06.048

Cited by 31 publications

(14 citation statements)

References 28 publications

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“…It has been reported that a network of pro-inflammatory cytokines, such as TNF-α and IL-1β, has a close relationship with the ALI model [16,17]. We found that isorhamnetin pretreatment restrained all of the LPS-induced increases in TNF-α and IL-1β in vivo.…”

Section: Discussionsupporting

confidence: 53%

Protective Effect of Isorhamnetin on Lipopolysaccharide-Induced Acute Lung Injury in Mice

Yang

et al. 2015

Inflammation

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Isorhamnetin has been reported to have anti-inflammatory, anti-oxidative, and anti-proliferative effects. The aim of this study was to investigate the protective effect of isorhamnetin on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice by inhibiting the expression of cyclooxygenase-2 (COX-2). The effects of isorhamnetin on LPS-induced lung pathological damage, wet/dry ratios and the total protein level in bronchoalveolar lavage fluid (BALF), inflammatory cytokine release, myeloperoxidase (MPO) and superoxide dismutase (SOD) activities, and malondialdehyde (MDA) level were examined. In addition, the COX-2 activation in lung tissues was detected by Western blot. Isorhamnetin pretreatment improved the mice survival rates. Moreover, isorhamnetin pretreatment significantly attenuated edema and the pathological changes in the lung and inhibited protein extravasation in BALF. Isorhamnetin also significantly decreased the levels of inflammatory cytokines in BALF. In addition, isorhamnetin markedly prevented LPS-induced oxidative stress. Furthermore, isorhamnetin pretreatment significantly suppressed LPS-induced activation of COX-2. Isorhamnetin has been demonstrated to protect mice from LPS-induced ALI by inhibiting the expression of COX-2.

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Section: Discussionsupporting

confidence: 53%

Protective Effect of Isorhamnetin on Lipopolysaccharide-Induced Acute Lung Injury in Mice

Yang

et al. 2015

Inflammation

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“…SOD is an anti-oxidative protein and treatments stimulating the production of SOD were proved to help to alleviate acute lung injury (29,30). Further studies showed that SOD exerted its protective effects by decreasing the quantity of neutrophiles in the systemic circulation and in alveoli, histological injury and oxidative stress (31).…”

Section: Discussionmentioning

confidence: 99%

Xuebijing injection attenuates pulmonary injury by reducing oxidative stress and proinflammatory damage in rats with heat stroke

Chen

Tong

Pan

et al. 2017

Experimental and Therapeutic Medicine

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The present study aimed to investigate the protective effect of Xuebijing injection (XBJ) on lung injury in heat-stroke rats and the underlying mechanisms. In total, 54 rats were randomly assigned to non-thermal, saline vehicle and XBJ groups. The rectal temperature (Tc), mean arterial pressure (MAP) and respiratory rate (RR) of the rats were recorded. The time-point of heat stroke and the time of survival were assessed, and indicators of arterial blood gas were regularly measured from 0 to 60 min. The concentration of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-10 was also determined. At the end of the experiment, lung tissue was harvested for histopathological analysis. Inducible nitric oxide synthase (iNOS) and superoxide dismutase (SOD) expression was measured by immunohistochemistry. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling was used to measure apoptosis. XBJ pretreatment prolonged the decline of clinical characteristics, as demonstrated by increases in Tc, MAP, RR and indicators in arterial blood gas in rats under heat stress. The time until heat stroke and the survival time in the Saline group were shorter than in rats treated with XBJ. The expression of iNOS in lung tissue and the concentration of TNF-α, IL-1β and IL-10 in the bronchoalveolar lavage fluid of rats treated with saline was higher than in rats with XBJ pre-treatment. Contrarily, SOD expression in rats treated with saline was decreased compared with that in rats treated with XBJ. Moreover, the apoptotic rate in the lung tissues of rats with saline treatment was higher than that in rats treated with XBJ. In conclusion, XBJ delayed the development of heat stroke and increased the survival time in rats under heat-stress by ameliorating pulmonary failure and acute lung injury. The underlying mechanisms of this effect may be the reduction of inflammatory cytokines as well as attenuation of oxidative stress and apoptosis by XBJ.

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“…In contrast, reducing oxidative stress demonstrates protective effects against diabetic complications 37 . DHI reduces oxidative stress by activating superoxide dismutase (SOD) and reducing production of malondialdehyde (MDA) in both myocardial ischemia/reperfusion (MI/R) and acute lung injury (ALI) mouse models 38 39 . Hydroxysafflor yellow A, salvianolic acid A/B and danshensu are the main active chemical constituents in DHI.…”

Section: Discussionmentioning

confidence: 99%

Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy

Pan

Chen

et al. 2015

Sci Rep

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Danhong Injection (DHI), a Chinese medicine for treatment of patients with coronary heart disease, inhibits primary abdominal aortic aneurysms in apoE deficient (apoE−/−) mice. Formation of microaneurysms plays an important role in the development of diabetic retinopathy and nephropathy. It remains unknown if DHI can reduce these diabetic complications. In this study, diabetic db/db mice in two groups were injected with saline and DHI, respectively, for 14 weeks. Blood and tissue samples were collected to determine serum glucose, lipids and tissue structure. DHI reduced diabetes-induced body weight gain, serum cholesterol and glucose levels. In retinas, DHI blocked the shrink of whole retina and retinal sub-layers by inhibiting expression of caspase 3, matrix metalloproteinase 2 (MMP-2) and MMP-9, accumulation of carbohydrate macromolecules and formation of acellular capillaries. DHI improved renal functions by inhibiting mesangial matrix expansion, expression of vascular endothelial growth factor A, fibronectin and advanced glycation end products in kidneys. Mechanistically, DHI induced expression of glucokinase, AMPKα/phosphorylated AMPKα, insulin receptor substrate 1, fibroblast growth factor 21 and peroxisome proliferator-activated γ. Expression of genes responsible for energy expenditure was also activated by DHI. Therefore, DHI inhibits diabetic retinopathy and nephropathy by ameliorating glucose metabolism and demonstrates a potential application in clinics.

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Preventive and therapeutic effects of Danhong injection on lipopolysaccharide induced acute lung injury in mice

Cited by 31 publications

References 28 publications

Protective Effect of Isorhamnetin on Lipopolysaccharide-Induced Acute Lung Injury in Mice

Protective Effect of Isorhamnetin on Lipopolysaccharide-Induced Acute Lung Injury in Mice

Xuebijing injection attenuates pulmonary injury by reducing oxidative stress and proinflammatory damage in rats with heat stroke

Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy

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