Prevention of ultraviolet radiation-induced immunosuppression by sunscreen in Candida albicans-induced delayed-type hypersensitivity

Abstract

Ultraviolet (UV) radiation-induced immunosuppression leading to skin cancer has received increased attention in previous years. The present study aimed to investigate the immunoprotection offered by Anthelios sunscreen in a mouse model of Candida albicans-induced delayed-type hypersensitivity. Anthelios sunscreen was applied to the skin on the dorsal skin of BALB/c mice treated with a sub-erythema dose of solar-simulated radiation. Delayed-type hypersensitivity was induced by immunization with Candida albicans. Changes in the skin thickness of the foot pads were measured, and immunosuppression rates were also evaluated. The expression levels of CD207, CD80 and CD86 in the Langerhans cells were semi-quantitatively detected using Western blotting and immunohistochemical assays. The delayed-type hypersensitivity mouse model was successfully established. The minimal erythema doses of UVA and UVB exposure to the mice were 2,000 and 145 mJ/cm2, respectively. The immunosuppression rates in the sunscreen group and non-sunscreen group were 24.39 and 65.85%, respectively (P<0.01). The results of the Western blotting and immunohistochemistry showed that the expression levels of CD207 (P<0.01), CD80 (P<0.05) and CD86 (P<0.01) were higher in the sunscreen group, compared with those in the non-sunscreen group. UV exposure reduced Candida albicans antigen-induced delayed-type hypersensitivity. Anthelios sunscreen was found to protect the skin from immunosuppression through the activation of epidermal Langerhans cells.

Nuclear and Urinary Measurements Show the Efficacy of Sun-Protection Factor 50+ Sunscreen against DNA Photoproducts upon Real-Life Recreational Exposure

“…Monitoring deleterious cellular events can be used as a more direct assessment of the protection afforded by sunscreen against UVR-induced skin carcinogenesis. For example, evidence has been provided for the prevention of immunosuppression by sunscreens ( Chen et al., 2016 ; Moyal and Fourtanier, 2008 ). In addition, several works have shown the reduced level of DNA photoproducts in the skin biopsies or roof of suction blisters of volunteers exposed to simulated solar UVR under laboratory conditions ( Bacqueville et al., 2015 ; Freeman et al., 1988 ; Josse et al., 2020 , 2018 ; van Praag et al., 1993 ; Young et al., 2000 , 2018 ).…”

Dysbiosis of skin mycobiome in atopic dermatitis

“…Positive skin prick tests for C. albicans were commonly seen in AD patients, especially in those with severe disease, and the severity of AD was found to be associated with the production of C. albicans ‐specific IgE antibodies 5,45 . C. albicans could also induce a delayed‐type hypersensitivity response on the skin by inducing the release of T cell co‐stimulatory molecules such as CD 207, CD 80 and CD86 46 . In evaluation of C. albicans ‐specific IgG levels, significantly lower levels of the yeast‐specific IgG were detected in AD patients, indicating the role of humoral immune response to Candida in the pathogenesis of AD 42 …”

Desenvolvimento de epiderme humana reconstruída (RHE) como plataforma de testes <i>in vitro</i> para irritação, sensibilização, dermatite atópica e fotoimunossupressão

“…Somado a isso, a RUV possui um papel imunossupressor, e, estudos usando ratos, demonstraram uma forte ligação entre os efeitos fotoimunossupressores e o desenvolvimento de câncer (RIVAS; ULLRICH, 1992). Contudo, não está claro como a RUV induz a fotoimunossupressão (CHEN et al, 2016;RIVAS;ULLRICH, 1992) e a literatura sugere que esses efeitos ocorrem devido ao envolvimento de fatores solúveis induzidos após a exposição a RUV (RIVAS; ULLRICH, 1992).…”

“…Em modelos animais uma única dose de RUV é capaz de causar imunossupressão e anulação da CHS (KIM; KRIPKE; ULLRICH, 1990). No entanto, a falta de métodos adequados para avaliação da imunossupressão permanece um desafio para estudos fotodermatológicos (CHEN et al, 2016).…”