Prevention of third molar tooth development in neonate rat with a long pulse diode laser

Silvestri, Anthony R.; Mirkov, Mirko; Connolly, Raymond J.

doi:10.1002/lsm.20093

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…Prevention of third molar eruption, if possible, will therefore reduce harm to humans. Thus far, researchers have used electrosurgical energy [3], long-pulse diode lasers [4], cryogenic techniques, sclerosing agents [5], citral [6], aspirin [7] and other techniques and agents to stop tooth development.…”

Section: Introductionmentioning

confidence: 99%

Dexamethasone inhibits BMP7-induced osteogenic differentiation in rat dental follicle cells via the PI3K/AKT/GSK-3β/β-catenin pathway

Tang

Qing

et al. 2020

Int. J. Med. Sci.

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Impacted third molars are commonly seen in teenagers and young adults and can cause considerable suffering. Preventing eruption of the third molars can reduce pain at the source. Our previous study has shown that dexamethasone (DEX) at a certain concentration can prevent the eruption of third molars without damaging alveolar bone in Sprague-Dawley (SD) rats, but the relevant molecular mechanisms need to be explored. This study aimed to explore the effects of high concentrations of DEX on osteogenic signaling pathways, including BMP/Smad and Wnt/β-catenin pathways, in rat dental follicle cells (rDFCs) and to elucidate the possible mechanisms. The results showed that BMP7 induced osteogenic differentiation by increasing the activity of ALP and the protein levels of OPN in rDFCs. DEX decreased endogenous BMP7 and phosphorylated Smad1/5/8 expression as well as BMP7-induced osteogenic differentiation. DEX also reduced the mRNA and protein levels of β-catenin by enhancing the expression of GSK-3β. In addition, regardless of DEX intervention, overexpression of BMP7 promoted the expression of β-catenin, while knockdown of BMP7 attenuated it. Further investigation revealed that overexpression of BMP7 attenuated the DEX-mediated inhibition of AKT and GSK-3β phosphorylation, but knockdown of BMP7 exerted the opposite effects. This study suggests that high concentrations of DEX may inhibit the expression of β-catenin via the PI3K/AKT/GSK-3β pathway in a manner mediated by BMP7. The findings further illustrate the possible molecular mechanisms by which DEX prevents tooth development.

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Section: Introductionmentioning

confidence: 99%

Dexamethasone inhibits BMP7-induced osteogenic differentiation in rat dental follicle cells via the PI3K/AKT/GSK-3β/β-catenin pathway

Tang

Qing

et al. 2020

Int. J. Med. Sci.

View full text Add to dashboard Cite

show abstract

“…In a previous report, diode laser energy was applied to the soft tissues overlying the sites of third molar development in neonate rats and third molars were selectively prevented from developing up to 80% of the time with little or no observable side effects [2]. This preliminary report describes developmental effects observed on mandibular third molars when several different doses of diode laser energy were applied to the mouths of a small number of young dogs at an age when third molars are just beginning to form.…”

Section: Introductionmentioning

confidence: 99%

“…Since heat kills cells and is an effective teratogen in developing tissues at temperatures as low as 5-68C above body temperature [7], the diode laser may be capable of killing third molar developmental cells. In a previous experiment, a 20 W diode laser with 800 nm wavelength applied to the soft tissues overlying the site of third molar development in young neonate rat pups stopped development of 80% of mandibular third molars with infrequent observable side effects [2].…”

Section: Introductionmentioning

confidence: 99%