Prevention of thioacetamide‐induced hepatotoxicity by biflavanones of <i>Garcinia kola</i>

Iwu, Maurice M.; Igboko, Ogo A.; Elekwa, Ogbonnaya K.; Tempesta, Michael S.

doi:10.1002/ptr.2650040407

Cited by 32 publications

(27 citation statements)

References 8 publications

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“…The animals in group 3 received curcumin (200 mg / kg /day) [18] in corn oil by gavage for the first 7 days followed by a daily administration of both curcumin and DBP concurrently for the last 9 days of the experiment. All the animals in group 4 received kolaviron (200 mg / kg /day) [9,19] dissolved in the vehicle and were also treated with DBP in the same manner as those of group 3. Twenty-four hours after the last treatment, all rats were sacrificed by cervical dislocation and blood was collected by cardiac puncture.…”

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confidence: 99%

Curcumin and Kolaviron Ameliorate Di‐n‐Butylphthalate‐Induced Testicular Damage in Rats

Farombi

Abarikwu

Adedara

et al. 2006

Basic Clin Pharma Tox

119

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The present study was carried out to evaluate the ameliorative effects of kolaviron (a biflavonoid from the seeds of Garcinia kola ) and curcumin (from the rhizome, Curcuma longa L.) on the di-n-butylphthalate (DBP)-induced testicular damage in rats. Administration of DBP to rats at a dose of 2 g / kg for 9 days significantly decreased the relative testicular weights compared to the controls, while the weights of other organs remained unaffected. Curcumin or kolaviron did not affect all the organ weights of the animals. While only DBP treatment significantly increased the testicular malondialdehyde level and gamma-glutamyl transferase activity ( γ -GT), it markedly decreased glutathione level, the testicular catalase, glucose-6-phosphate dehydrogenase, superoxide dismutase, sperm γ -GT activities and serum testosterone level compared to the control group. Data on cauda epididymal sperm count and live/dead ratio were not significantly affected in the DBPtreated rats. Alone, DBP treatment resulted in a 66% decrease in spermatozoa motility and a 77% increase in abnormal spermatozoa in comparison to control. DBP-treated rats showed marked degeneration of the seminiferous tubules with necrosis and defoliation of spermatocytes. The DBP-induced injuries in biochemical, spermatological parameters and histological structure of testis were recovered by treatment with kolaviron or curcumin. The pattern in the behaviour of these compounds might be correlated with their structural variations. Our results indicate that kolaviron and curcumin protect against testicular oxidative damage induced by DBP. The chemoprotective effects of these compounds may be due to their intrinsic antioxidant properties and as such may prove useful in combating phthalate-induced reproductive toxicity.Benzenedicarboxylic acid dibutyl ester, also known as butylphthalate or di-n-butylphthalate (DBP) ( fig. 1A), is widely used as a plasticizer in cellulose plastics, as a solvent for dye, and for a variety of consumer products [1]. Release of DBP to the environment can occur during production and also during incorporation of the phthalate into plastics. Because DBP is not bound to the final product, it can be released during use or disposal of the product. Thus, the general population is exposed to phthalates through consumer products, diet and medical devices [2].Phthalate esters have been implicated in the decline of human sperm counts [3] and testicular atrophy in an animal model [4]. The exact mechanisms involved in the formation of phthalate-induced testicular atrophy are not known, but recently it was shown that administration of antioxidant vitamins together with di-ethyl-hexylphthalate prevented phthalate ester-induced testicular atrophy in rats [5] suggesting that the formation of reactive oxygen species may be involved in the formation of testicular atrophy in phthalate-exposed rats.Curcumin obtained from the Curcuma longa rhizome is the major component of turmeric used as a spice to give the specific flavour and yellow colour to cu...

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confidence: 99%

Curcumin and Kolaviron Ameliorate Di‐n‐Butylphthalate‐Induced Testicular Damage in Rats

Farombi

Abarikwu

Adedara

et al. 2006

Basic Clin Pharma Tox

119

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“…Removal of the solvent in vacuo in a rotary evaporator provided an organic extract (600g). Kolaviron was isolated according to Iwu et al 3 as modified by Farombi et al 4 Briefly, the powdered seeds were extracted with light petroleum ether (b.pt 40-60 o C) in a soxhlet for 24 h. The defatted, dried marc was repacked and extracted with acetone (Me 2 CO). The extract was concentrated and diluted twice its volume with water and extracted with ethyl acetate.…”

Section: Plant Material Preparation Of Extract Fractions and Compoundsmentioning

confidence: 99%

Bio-flavonoids and Garcinoic Acid from Garcinia kola seeds with Promising Anti-Inflammatory Potentials

Michel¹,

Ottoh²,

Chukwunonye³

et al. 2015

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Objective: The research was carried out to investigate the anti-inflammatory effect of ethanol extract, fraction (kolaviron) and compounds (garcinoic acid, GB1 and GB2) of Garcinia kola seeds. Materials and Method: To evaluate the acute anti-inflammatory effect of extract, fraction and compounds of G. kola carrageenan-induced edema model in wistar albino rats was used. Results: Kolaviron (50 mg/kg), garcinoic acid (50 mg/kg) and the crude extract (50 mg/kg) caused 100, 83 and 74% inhibition of carrageenan-induced paw edema respectively at 6 h post administration. Indomethacin (10 mg/ kg), the reference drug induced 100% inhibition of carrageenan-induced paw oedema. While GB1 (50 mg/kg) and GB2 (50 mg/kg) was prominent at 4, 5 and 6 h post administration. Conclusion: Results showed that the extract possessed anti-inflammatory activity, which have justified their use in Nigeria traditional medicine to treat inflammation.

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“…In vivo experiments presented biflavonoids as hepatoprotective, increased RNA synthesis in hepatocytes inhibited expression of EBV gene in addition to anti-spasmogenic activities and antibradykinin [13][14][15][16]. Recently, it was shown that biflavonoids are effective in the management of tuberculosis and postmenopausal breast cancer [4][5][6][7][8][9][10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, it was shown that biflavonoids are effective in the management of tuberculosis and postmenopausal breast cancer [4][5][6][7][8][9][10][11][12][13][14][15][16][17]. Two TLC chromatographic methods had been used to separate and determine biflavonoids and flavonoids from C. sempervirens L. [18].…”

Section: Introductionmentioning

confidence: 99%

“…Two TLC chromatographic methods had been used to separate and determine biflavonoids and flavonoids from C. sempervirens L. [18]. Although many biflavonoids have been reported in the literature, complete identification and quantitation were unsatisfying [6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Romani et al [7] reported the first determination and quantification of the main individual polyphenols; flavonoid glycosides and biflavonoids in the leaf tissues from different Cupressus species using HPLC-DAD, HPLC-MS, and HPTLC.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Cupressuflavone and Amentoflavone from Cupressus sempervirens L. and its Tissue Cultured Callus using HPLC-DAD Method

ElSohly¹

2017

PPIJ

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An HPLC-DAD method has been optimized and validated to obtain qualitative and quantitative profiles of cupressuflavone, and amentoflavone from Cupressus sempervirens L. compared to the content of micropropagated plants. The plants analyzed were collected from two geographical sources in Egypt; Cupressus from the North coast and Cupressus from Future University (Cairo, Egypt). Isocratic reversed phase HPLC separation was achieved using a C 18 column (150 mm× 4.6 mm I.D., 5μm). Good resolution between cupressuflavone and amentoflavone was achieved using a mixture of water: acetonitrile: formic acid (60: 40: 0.1%, v/v) as a mobile phase at a flow rate of 1mLmin -1. Quantitation was achieved with photodiode array detector at the wavelength of maximum absorbance (330 nm) based on peak area. The method was effective in the determination of the analyses of interest without any interference of other compounds or the matrix. The method was linear over a concentration range of 0.5-40 μg mL -1 (r 2 > 0.999) for biflavonoids. The limits of detection were 0.15 and 0.14 μg mL -1 and the limits of quantitation were 0.47 and 0.43 μg mL -1 for cupressuflavone and amentoflavone, respectively. Intraday and Interday precision (RSD %) were less than ±3%. Intraday and Interday accuracy (RE %) of the method was found to be less than ±15 % at all concentrations tested. The procedure was relatively simple, precise, rapid, reproducible, and could be routinely used for analysis of the cupressuflavone and amentoflavone flavonoids.

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Prevention of thioacetamide‐induced hepatotoxicity by biflavanones of Garcinia kola

Cited by 32 publications

References 8 publications

Curcumin and Kolaviron Ameliorate Di‐n‐Butylphthalate‐Induced Testicular Damage in Rats

Curcumin and Kolaviron Ameliorate Di‐n‐Butylphthalate‐Induced Testicular Damage in Rats

Bio-flavonoids and Garcinoic Acid from Garcinia kola seeds with Promising Anti-Inflammatory Potentials

Analysis of Cupressuflavone and Amentoflavone from Cupressus sempervirens L. and its Tissue Cultured Callus using HPLC-DAD Method

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