Prevention of Secondary Caries Using Resin-Based Pit and Fissure Sealants Containing Hydrated Calcium Silicate

Abstract

The objective of this study was to investigate the effects of hydrated calcium silicate filler (hCS) on resin-based pit and fissure sealants’ acid neutralization, calcium ion release, and mechanical and physical properties. To produce the hCS filler, Portland cement (CS) was mixed with distilled water and ground into fine particles. The particles were then mixed with silanized glass filler and added to a photo-activated resin matrix. To evaluate the acid neutralization and calcium ion release properties, the specimens were immersed in a pH 4.0 lactic acid solution and distilled water for 28 days. Also, the flexural strength, depth of cure, water sorption, and solubility were tested. All of the groups containing hCS and CS required less than one minute to increase the pH from 4.0 to 5.5. With 50% hCS, the calcium ion release was higher than 50% CS in the distilled water at the initial time. The flexural strength and depth of cure decreased according to the increasing proportion of hCS added. The water sorption and solubility had an increasing trend as increasing proportions of hCS were added. These findings showed that pit and fissure sealant containing hCS exhibit superior acid neutralization and calcium release properties, and may be promising for caries-inhibiting dental material.

Assessment of Water Sorption, Solubility and Interface Properties for Two Different Pit and Fissure Sealants

“…The results showed that, there were significant differences between the sorption values after 14 and 21 days, for both dental sealants. These observations are in addition to the conclusions of other studies on the complete lack of predictability of the time period regarding the water absorption of dental materials [18].…”

“…Some of the absorbed water act as a plasticizer and affect mechanical properties of resinbased materials. It is observed that the materials with high absorption also have high solubility values at the same measurement interval [9,18,19].…”

“…Although the ISO 4049/2019 standard recommends a test period of 7 days, in this study we extended the evaluation to 14 and then to 21 days, for a better characterisation of the time factor on absorption and solubility [18].…”

Dentine sialophosphoprotein signal in dentineogenesis and dentine regeneration

“…SHEDs have a high proliferation rate and can differentiate into adipogenic, chondrogenic, myogenic, neurogenic, odontogenic and osteogenic cells in vitro as well as induce formation of dentine and bone in vivo (Miura et al, 2003). SHEDs, neuralcrest-derived stem cells, also express neural cell markers such as nestin, beta III tubulin and GFAP as well as several pluripotent markers including Oct4 and Nanog (Chai et al, 2000;Miura et al, 2003;Yang et al, 2019;Yang et al, 2020b). SHEDs express more osteocyte markers such as ALP, collagen type I and Runx2 than do BM MSCs in vitro.…”

“…SCAPs are optimised for osteogenesis and odontogenesis; regarding that, SCAPs are considered to be odontoblast precursors in vivo (Du et al, 2020;Nada and El Backly, 2018). However, SCAPs are multipotent and give rise to mesenchymal cell lineages such as adipocytes and chondrocytes (Yang et al, 2020b). Taken together, SCAPs will hopefully gain a significant role in tissue repair and regeneration.…”