Prevention of primary nonfunction after canine liver allotransplantation: The effect of gadolinium chloride

Chung, Ku Yong; Jeong, Gyu-Young; Choi, Ki-Yeul; Sung, Sun-Hee; Kim, Yu Seun

doi:10.1016/j.transproceed.2004.09.006

Cited by 5 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GdCl 3 can suppress a broad range of macrophage-related cell markers and cytokines, including TNF-α, IL-6, and IL-1B [32, 33]. At the same time, GdCl 3 can reduce the levels of peroxisomal protein (PXP), CYP311, cytochrome P450, and many functional mRNAs [34–37]. Systemic knockout of IL-6 in mice has improved our understanding of the role of IL-6 in hepatic pathophysiology to a greater degree than GdCl 3 .…”

Section: Discussionmentioning

confidence: 99%

Deletion of interleukin-6 in monocytes/macrophages suppresses the initiation of hepatocellular carcinoma in mice

Kong

Zhou

et al. 2016

J Exp Clin Cancer Res

124

View full text Add to dashboard Cite

BackgroundHepatocellular carcinoma (HCC) is associated with inflammation, and roughly 30 % of the global population shows serological evidence of current or past infection with hepatitis B or hepatitis C virus. Resident hepatic macrophages, known as Kupffer cells (KCs), are considered as the specific tumor-associated macrophages (TAMs) of HCC, and can produce various cytokines—most importantly interleukin (IL)-6—to promote tumorigenesis of HCC. However, the roles of KCs and IL-6 in carcinogenesis in the liver are still unclear.MethodsWe analyzed leukocyte-related peripheral blood data of 192 patients and constructed a mouse model in which the bone marrow was cleared out by irradiation and reconstructed using bone marrow donated from IL-6-deficient mice to further elucidate the hepatic pathological changes in response to toxic challenge and oncogenic gene mutation.ResultsPeripheral monocyte counts and serum IL-6 levels were significantly higher in patients with HCC than in those without HCC. In addition, there was a significant difference in the levels of IL-6 among individuals with different histopathological grades. In mice with selective IL-6 ablation in monocytes/KCs, we observed decreased toxic liver injury, inflammatory infiltration, and systemic inflammation. In Mdr2-deficient mice, which spontaneously developed HCC, the loss of IL-6 in monocytes/KCs resulted in inhibition of IL-6/signal transducer and activator of transcription 3 signaling, decreased serum IL-6 levels, and delayed tumorigenesis.ConclusionsOur findings demonstrate that increased TAM-derived IL-6 had an amplifying effect on the inflammation response, thereby promoting the occurrence and development of HCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Deletion of interleukin-6 in monocytes/macrophages suppresses the initiation of hepatocellular carcinoma in mice

Kong

Zhou

et al. 2016

J Exp Clin Cancer Res

124

View full text Add to dashboard Cite

show abstract

“…However, extensive investigations are needed to address the challenges related to the interference of GdCl 3 with magnetic resonance imaging of paramagnetic nanoparticles since GdCl 3 does alter proton T 1 and T 2 relaxation, albeit to a different extent than gadolinium chelated to agents like DTPA. Second, this approach has been undertaken extensively in preclinical animal models of liver transplantation, where engraftment of transplanted cells is improved in the presence of GdCl 3 that inhibits Kupffer cell mediated hepatocyte transplantation-induced liver inflammation and ischemia−reperfusion injury. − GdCl 3 has been generally regarded as safe in these preclinical models. Third, reducing the retention of QDs within the liver reduces the probability of deterministic late side effects, an area of concern for QD biocompatibility in clinical use.…”

Section: Resultsmentioning

confidence: 99%

Gadolinium Chloride Augments Tumor-Specific Imaging of Targeted Quantum Dots In Vivo

et al. 2010

View full text Add to dashboard Cite

Non-specific sequestration of nanoparticles by the reticulo-endothelial system (RES) results in the degradation of image quality of nanoparticle-based imaging. We demonstrate that Gadolinium chloride (GdCl3) pretreatment inactivates RES macrophages thereby increasing circulatory time and amplifying tumor-specific signal of conjugated nanoparticles in vivo. The experimental results were validated using compartmental modeling and the rate parameters for the observed kinetics pattern were estimated. This pretreatment strategy could have broad applicability across biomedical applications utilizing theranostic nanoparticles that are sequestered by the RES.

show abstract

“…During liver resection, hepatic blood flow is often occluded in order to control bleeding. Most surgeons perform selective semi-hepatic blood flow occlusion when possible [16], but extensive hepatic cancer may require excision of the whole liver block, which inevitably leads to ischemia reperfusion (IR) injury [17,18].…”

Section: Discussionmentioning

confidence: 99%

Somatostatin Octapeptide Inhibits Cell Invasion and Metastasis in Hepatocellular Carcinoma Through PEBP1

Huang

Liu

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Hepatocellular carcinoma (HCC) is a major threat to human health. The condition carries a high risk of death; 45% of new cases occur in China. Surgical resection is the first choice for treatment of HCC, but 30.9% of patients experience recurrence within 6 months after the operation. To improve patient survival, we must determine how to reduce the probability of recurrence and metastasis and elucidate the underlying mechanism of disease. We therefore studied the effect of somatostatin octapeptide (octreotide) on the invasion and metastasis of HCC. Methods: The migration and invasion cytological tests were used to detect the effect of octreotide on liver cancer cells (SK-Hep-1 and HepG2). PEBP1 RNAi was used to knockdown expression. Invasion and metastasis were measured with transwell migration and wound-healing assays. Western blotting was used to detect changes in levels of PEBP1 and invasion pathway proteins after octreotide treatment. The effect of octreotide was studied in vivo by establishing a pulmonary metastasis model using SK-Hep-1 cells in nude mice. In-vivo bioluminescence imaging and hematoxylin and eosin staining of lung tissue were used to verify the results. Results: Increasing concentrations of octreotide were progressively more effective in halting the invasion and metastasis of liver cancer cells. Octreotide may upregulate PEBP1, TIMP-2, and E-cadherin while downregulating MMP-2 and Twist to inhibit cell invasion and metastasis. And downregulation of PEBP1 would also change the expression of MMP-2, TIMP-2 and Twist. The in-vivo experiments showed no cancer cell metastasis in 4 of the 6 mice in the octreotide-treatment group, while all of the mice in the control group displayed pulmonary metastasis of human HCC cells. And the survival period of the mice in the octreotide-treatment group was significantly prolonged. Conclusions: Octreotide may weaken invasion and metastasis through the upregulation of PEBP1. Octreotide may reduce the risk of recurrence and metastasis after surgery for liver cancer.

show abstract

Prevention of primary nonfunction after canine liver allotransplantation: The effect of gadolinium chloride

Cited by 5 publications

References 9 publications

Deletion of interleukin-6 in monocytes/macrophages suppresses the initiation of hepatocellular carcinoma in mice

Deletion of interleukin-6 in monocytes/macrophages suppresses the initiation of hepatocellular carcinoma in mice

Gadolinium Chloride Augments Tumor-Specific Imaging of Targeted Quantum Dots In Vivo

Somatostatin Octapeptide Inhibits Cell Invasion and Metastasis in Hepatocellular Carcinoma Through PEBP1

Contact Info

Product

Resources

About