Prevention of onset in an insulin‐dependent diabetes mellitus model, NOD mice, by oral feeding of Lactobacillus casei

Abstract

Prevention of onset in an insulin-dependent diabetes mellitus model, NOD mice, by oral feeding of Lactobacillus casei. APMIS 105: [643][644][645][646][647][648][649] 1997.The effects of Lactobacillus casei (LC) on the onset of diabetes in an insulin-dependent diabetes mellitus model, nonobese diabetic (NOD) mice, were examined. From the age of 4 weeks, female NOD mice were fed a diet of either standard laboratory chow (n= 12) or the same chow containing 0.05% weight heat-killed cells of LC (n= 12), and the onset of diabetes was thereafter recorded. The incidence of diabetes in the control group (10112) was significantly higher than that in the LC-treated group (31 12) (p<O.Ol). Pathological analysis in the LC-treated group revealed strong inhibition of the disappearance of insulin-secreting fl cells in Langerhans islets caused by autoimmune disease. The proportion of CD45R+ B-cells in the spleen was increased and that of CD8+ T-cells in spleen cells was decreased in the LC-treated group. Analysis of cytokine production revealed lower interferon-y production in the LC-treated group compared to the control group, while the interleukin (IL)-2 production was higher. The levels of IL-4, 1L-5, IL-6 and IL-10 in the LC-treated group were somewhat higher than in the control group. Taken together, these findings clearly demonstrated that oral feeding of LC to NOD mice effectively inhibited the occurrence of diabetes and regulated the host immune responses.

Lectin Microarray Reveals Binding Profiles of Lactobacillus casei Strains in a Comprehensive Analysis of Bacterial Cell Wall Polysaccharides

“…While the bacterium is known to be a strong Th1-type cytokine inducer (32), of which the active component was proposed to be LTA (12), it exerts anti-inflammatory activity against diabetes mellitus (26) and inflammatory bowel disease (24,25) in animal models. It was shown that the cell wall preparation containing PS and PG could be the active component of the anti-inflammatory activity (25).…”

Flexible cytokine production by macrophages and T cells in response to probiotic bacteria: A possible mechanism by which probiotics exert multifunctional immune regulatory activities

“…Improvement of the Th1/Th2 balance and downregulation of IL-6 production were proposed to be the underlying mechanisms. 22,23 LcS has also been shown to be effective in experimental models of autoimmune diseases such as arthritis, 24 type I diabetes 25 and systemic lupus erythematosus. 26 These multifactorial clinical efficacies suggest the possibility that LcS can not only augment host immune defense against cancer and infection, been used for making fermented dairy products for more than 70 years.…”

Toward defining the autoimmune microbiome for type 1 diabetes

“…A role for bacteria in the onset of diabetes has been shown in two murine models. For example, feeding probiotic bacterial strains, usually lactic acid bacteria, to non-obese diabetic mice or biobreeding diabetesprone (BB-DP) rats can delay or prevent diabetes (Matsuzaki et al, 1997;Calcinaro et al, 2005;Yadav et al, 2007). Feeding antibiotics to nonobese diabetic mice or BB-DP rats can also increase survival in these models Schwartz et al, 2007).…”