Prevention of liver ischemia reperfusion injury by a combined thyroid hormone and fish oil protocol

Mardones, Marcelo; Valenzuela, Rodrigo; Romanque, Pamela; Covarrubías, Natalia; Anghileri, Fiorella; Fernández, Virgínia; Videla, Luis A.; Tapia, Gladys

doi:10.1016/j.jnutbio.2011.06.004

Cited by 33 publications

(33 citation statements)

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“…Interestingly, there is evidence to conclude that n-� LCPUFAs potentiate the effects of certain drugs, thereby allowing a reduction of their required dose, thus avoiding adverse effects [48] . In agreement with this view, combined n-3 LCPUFA (300 mg/kg for 3 consecutive days) plus T� (0.05 mg/kg) administration prevented rat liver IR injury, whereas separate protocols lack protection [107] , when compared with the preconditioning action afforded by separate n-� LCPUFA (300 mg/kg for 7 consecutive days) [27] or T� (0.1 mg/kg) [26] . Data discussed in this article warrants further experimental and clinical research in the future, to support the incorporation of T�, n-3 LCPUFA and iron preconditioning strategies or their combinations in human liver resections and in human liver transplantation using reduced-size grafts from living donors.…”

Section: Discussionmentioning

confidence: 68%

Recent advances in liver preconditioning: Thyroid hormone, n-3 long-chain polyunsaturated fatty acids and iron

Fernández

Tapia²,

Videla³

2012

WJH

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Liver preconditioning (PC), defined as an enhanced tolerance to injuring stimuli induced by previous specific maneuvers triggering beneficial functional and molecular changes, is of crucial importance in human liver transplantation and major hepatic resection. For these reasons, numerous PC strategies have been evaluated in experimental models of ischemia-reperfusion liver injury, which have not been transferred to clinical application due to side effects, toxicity and difficulties in implementation, with the exception of the controversial ischemic PC. In recent years, our group has undertaken the assessment of alternate experimental liver PC protocols that might have application in the clinical setting. These include thyroid hormone (T3), n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA), or iron, which suppressed liver damage due to the 1 h ischemia-20 h reperfusion protocol. T3, n-3 LCPUFA and iron are hormetic agents that trigger biologically beneficial effects in the low-dose range, whose multifactorial mechanisms of action are discussed in the work.

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Section: Discussionmentioning

confidence: 68%

Recent advances in liver preconditioning: Thyroid hormone, n-3 long-chain polyunsaturated fatty acids and iron

Fernández

Tapia²,

Videla³

2012

WJH

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“…Algunos estudios han empleado aceites de pescado como estrategia no invasiva contra lesiones de isquemia y reperfusión en hígado de rata y para la reducción del estrés oxidativo en hígado de ratas (Mardones et al, 2012;Valenzuela et al, 2011), con la información del presente trabajo se podrían seleccionar las especies con mayor contenido de estos ácidos grasos para sustituir el empleo de aceites de pescado. En el caso de estudios clínicos, el empleo de los pescados aquí analizados proporcionaría no sólo el beneficio de los ácidos grasos sino también el de su proteína de alto valor biológico, sus vitaminas y minerales, así como su bajo contenido de colesterol.…”

Section: áCidos Grasos Poliinsaturados (Agp)unclassified

Perfil de ácidos grasos de diversas especies de pescados consumidos en México

González

Rodríguez

Galindo-Gómez

2013

RBT

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Fatty acids in different edible fish species from Mexico. Different biotic and abiotic factors determine the fatty acid (FA) composition of fish tissues and organs. This information is useful for humans due to the fact that fish consumption is associated with health benefits. The aim of the present study was to identify the variation in the concentration of fatty acids, according to different factors, among ten edible marine fish species in Mexico, collected from June to December 2009 in the largest fish market in Mexico city: Euthynnus alletteratus, Sciaenops ocellatus, Bairdiella chrysoura, Sphyraena guachancho, Symphurus elongatus, Istiophorus platypterus, Ophichthus rex, Eugerres plumieri, Eucinostomus entomelas and Oreochromis mossambicus. Lipid content was gravimetrically quantified, the fatty acids were determined using a gas chromatograph and the results were statistically analyzed. Total lipid content ranged from 0.93 to 1.95g/100g in E. entomelas and O. urolepis hornorum, respectively. E. alletteratus, B. chrysoura, S. elongatus, I. platypterus, O. rex and E. plumieri presented the following order in FA concentration: Polyunsaturated FA (PUFA)>Saturated FA (SFA)>Monounsaturated FA (MUFA). S. ocellatus, S. guachancho and E. entomelas presented SFA>PUFA>MUFA; and only O. mossambicus presented SFA>MUFA>PUFA. O. mossambicus had the highest concentration (mg/100g) of SFA (559.40) and MUFA (442.60), while B. chrysoura presented the highest content (mg/100g) of PUFA (663.03), n-3 PUFA (514.03), EPA+DHA (506.10) and n-6 PUFA (145.80). Biotic and abiotic factors of the analyzed fish significantly influenced their FA concentration. Subtropical species presented 42.1% more EPA+DHA than tropical specie. Values presented here will vary according to the changes in the ecosystem and characteristics of each fish species, however the information generated in the present study is useful for improving fish consumption recommendations. Rev. Biol. Trop. 61 (4): 1981-1998. Epub 2013 December 01.Key words: fatty acids, marine fish, biotic factors, abiotic factors, Mexico.Los lípidos de la dieta influyen en el metabolismo, crecimiento y bienestar de los animales (Sargent, Bell, Bell, Henderson & Tocher, 1993;Sargent, Bell, McEvoy, Tocher & Estevez, 1999;Steffens, 1997). Se requieren para proveer energía y producir fosfolípidos y lípidos polares fundamentales para la formación de la membrana celular. En los peces, los lípidos son la principal fuente de energía para su crecimiento, especialmente durante el desarrollo embrionario; y son esenciales para su desarrollo neural, por lo que es común que en su cerebro y retina se encuentren altas concentraciones de ácido docosahexaenoico (C22:6n-3, DHA), ácido graso (AG) del tipo n-3 (Sargent et al., 1993). La ingesta de AG también determina el metabolismo de algunas enzimas que participan en la biosíntesis de los ácidos grasos endógenos en el caso de los peces de agua dulce, pues éstos son capaces de convertir algunos ácidos grasos poliinsaturados (AGP) (EPA (C20:5n-3, á...

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“…Taking in consideration BCL2L1 a product of an anti-apoptotic gene as well as BAX, a pro-apoptotic gene, the susceptibility of cells toward apoptotic stimuli can be defined [8,9,11]. Superoxide dismutase (SOD) encodes for an enzyme that converts superoxide radicals to hydrogen peroxide [12], gluthatione synthetase (GSS) encodes for a key enzyme in prevention of local oxidative stress [13,14], the peroxisome proliferators-activated receptor-alpha (PPARalpha) gene plays a role in energy metabolism and might suppress induction of apoptosis [15], the gene for oxidative stress responsive 1 (OXSR1) controls cell proliferation and cell death by apoptosis [16], gluthatione peroxidase 3 (GPX3) encodes for a cellular anti-oxidant system that protects cells against oxidative stress [17,18] and heat shock protein 70.2 gene expression (HSP 70.2) takes part in processes of protection and repair of stress-induced protein damage [19,20] in the different tissues. The aim of this study was to study a panel of genes involved in oxidative stress and apoptosis and correlate gene expression with immunohistochemical findings of apoptosis and nitrosative stress and serum markers of oxidative stress before and after harvesting of organs from a BD and LD pig model and after cold ischemia time (CIT) in order to have the potential possibility to find novel strategies for donor pre-conditioning and amelioration in further experiments.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

et al. 2013

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BackgroundAs organ shortage is increasing, the acceptance of marginal donors increases, which might result in poor organ function and patient survival. Mostly, organ damage is caused during brain death (BD), cold ischemic time (CIT) or after reperfusion due to oxidative stress or the induction of apoptosis. The aim of this study was to study a panel of genes involved in oxidative stress and apoptosis and compare these findings with immunohistochemistry from a BD and living donation (LD) pig model and after cold ischemia time (CIT).MethodsBD was induced in pigs; after 12 h organ retrieval was performed; heart, liver and kidney tissue specimens were collected in the BD (n = 6) and in a LD model (n = 6). PCR analysis for NFKB1, GSS, SOD2, PPAR-alpha, OXSR1, BAX, BCL2L1, and HSP 70.2 was performed and immunohistochemistry used to show apoptosis and nitrosative stress induced cell damage.ResultsIn heart tissue of BD BAX, BCL2L1 and HSP 70.2 increased significantly after CIT. Only SOD2 was over-expressed after CIT in BD liver tissue. In kidney tissue, BCL2L1, NFKB, OXSR1, SOD2 and HSP 70.2 expression was significantly elevated in LD. Immunohistochemistry showed a significant increase in activated Caspase 3 and nitrotyrosine positive cells after CIT in BD in liver and in kidney tissue but not in heart tissue.ConclusionThe up-regulation of protective and apoptotic genes seems to be divergent in the different organs in the BD and LD setting; however, immunohistochemistry revealed more apoptotic and nitrotyrosine positive cells in the BD setting in liver and kidney tissue whereas in heart tissue both BD and LD showed an increase.

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Prevention of liver ischemia reperfusion injury by a combined thyroid hormone and fish oil protocol

Cited by 33 publications

References 32 publications

Recent advances in liver preconditioning: Thyroid hormone, n-3 long-chain polyunsaturated fatty acids and iron

Recent advances in liver preconditioning: Thyroid hormone, n-3 long-chain polyunsaturated fatty acids and iron

Perfil de ácidos grasos de diversas especies de pescados consumidos en México

Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

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