Prevention of Lethal Osmotic Injury to Cells During Addition and Removal of Cryoprotective Agents: Theory and Technology

Gao, Dayong; Zhou, Xiaoming

doi:10.5772/34473

Cited by 6 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another important deleterious effect of cryopreservation is osmotic stress, which can also cause variations in the dimensions of the sperm head [14]. It has been suggested that over-condensation of sperm chromatin, damage to the plasma membrane and acrosome, as well as acrosome loss and damage to the cell cytoskeleton, could cause a reduction in the size of the head [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge

A. Galarza,

Duma,

X. Samaniego

et al. 2023

Veterinary Medicine and Science

View full text Add to dashboard Cite

Current sperm cryopreservation protocols for domestic and wild mammals aim to minimize the cryogenic damage caused by cell dehydration, ice formation, and osmotic stress. The optimization of sperm cryopreservation include the use of different synthetic and nonsynthetic-based extenders supplemented with additives (e.g., egg yolk, coconut water, etc.) and antioxidants (e.g., melatonin, L-carnitine, caffeine, resveratrol, etc.) that protect the plasmalemma, acrosome, and mitochondria against the detrimental effects caused by the cryopreservation process. Furthermore, the use of penetrating (e.g., glycerol, ethylene glycol, dimethylformamide, etc.) and nonpenetrating (e.g., sucrose and trehalose) cryoprotectant agents (CPAs) or their combination should be investigated to protect sperm during the freezing process in slow and ultra-rapid freezing procedures. Finally, new cryopreservation protocols should focus on freezing curves and initial cooling rates that allow optimal dehydration during freezing and adequate hydration during thawing. The suitable interaction of all these factors will allow a sperm subpopulation to survive cryopreservation with integrity and fertilizing capacity, contributing to the improvement of the efficiency of genetic resource management and the development of germplasm banks that support the preservation of genetic diversity in domestic and wild animals.

show abstract

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge

A. Galarza,

Duma,

X. Samaniego

et al. 2023

Veterinary Medicine and Science

View full text Add to dashboard Cite

show abstract

“…Repeated freezing–thawing does, however, increase the risk of the loss of viability. Osmotic stress is suffered when glycerol and water cross the sperm cell plasma membrane during both freezing and thawing (Gao & Zhou, ), which reduces the dimensions of the sperm head (Esteso et al ., ). Repeating this leads to further modifications of sperm morphometry with consequent alterations in functionality.…”

Section: Introductionmentioning

confidence: 99%

Giant panda (Ailuropoda melanoleuca) sperm morphometry and function after repeated freezing and thawing

et al. 2015

View full text Add to dashboard Cite

This work examines the effects of subsequent cycles of freezing-thawing on giant panda (Ailuropoda melanoleuca) sperm morphometry and function, and assesses whether density-gradient centrifugation (DGC) can increase the number of freezing-thawing cycles this sperm can withstand. A sperm sample was collected by electroejaculation from a mature giant panda and subjected to five freezing-thawing cycles. Although repeated freezing-thawing negatively affected (P < 0.05) sperm motility and membrane integrity, in both nonselected and DCG-selected sperm samples, >60% of the sperm cells in both treatments showed acrosome integrity even after the fifth freezing cycle. In fresh semen, the sperm head length was 4.7 μm, the head width 3.6 μm, area 14.3 μm(2) and perimeter length 14.1 μm. The present results suggest that giant panda sperm trends to be resistant to repeated freezing-thawing, even without DGC selection.

show abstract

“…This is to reduce the metabolic activity of spermatozoa (Dong et al 2008). Also, osmotic adaptation helps cryoprotectants entering the spermatozoa membrane (Gao and Zhou 2012). The equilibration time is required for the semen on matching themselves with an extender to prevent the death of spermatozoa on the freezing process (Tolihere 1993).…”

Section: Introductionmentioning

confidence: 99%

The effect of equilibration time on semen freezing of local swamp buffalo (Bubalus bubalis) with combination extender of lactose and glycerol

et al. 2017

View full text Add to dashboard Cite

Abstract. Eriani K, Sari N, Ihdina M, Rosnizar. 2017. The effect of equilibration time on semen freezing of local swamp buffalo (Bubalus bubalis) with combination extender of lactose and glycerol. Nusantara Bioscience 9: 77-82. This study was to determine the different time of equilibration on semen of swamp buffalo (Bubalus bubalis) which was diluted by adding Tris-Egg Yolk extender of lactose and glycerol cryoprotectant combination. Fresh sperm of the swamp buffalo (B. bubalis) was diluted by using Tris-Egg Yolk extender of lactose cryoprotectants combination 0 mM (L 0 ), 60 mM (L 60 ), 120 mM (L 120 ) and glycerol 3% (G 3 ), 5% (G 5 ), 7% (G 7 ) with the equilibration of 2,5, 3, and 4 hours. The parameter used in this study was the percentage of sperm motility and live treatment. The results of the 2.5-hour equilibration with a combination of cryoprotectants L 60 G 7 showed the best quality in all parameters. The results of the 3-hour equilibration with combination of cryoprotectants L 60 G 7 showed the best quality in all parameters whereas the best quality in all parameters in 4-hour equilibration was the combination of cryoprotectants L 120 G 7 .

show abstract

Prevention of Lethal Osmotic Injury to Cells During Addition and Removal of Cryoprotective Agents: Theory and Technology

Cited by 6 publications

References 54 publications

Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge

Cryopreservation of Domestic and Wild Animal Spermatozoa: Update of Knowledge

Giant panda (Ailuropoda melanoleuca) sperm morphometry and function after repeated freezing and thawing

The effect of equilibration time on semen freezing of local swamp buffalo (Bubalus bubalis) with combination extender of lactose and glycerol

Contact Info

Product

Resources

About