Prevention of Keratocyte Loss After Corneal Deepithelialization in Rabbits

Nassaralla, Belquiz; Szerenyi, Karin; Pinheiro, M.; Wee, Won Ryang; Nigam, Alok; McDonnell, Peter J.

doi:10.1001/archopht.1995.01100040128038

Cited by 11 publications

(5 citation statements)

References 26 publications

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“…As described by Nakayasu, these cellular degenerative changes were visible under transmission electron microscopy as early as 15 min after scraping [21]. Furthermore, it was reported that removal of corneal epithelium causes loss of superficial stromal keratocytes [5,6,22]. In the cases of penetrating injury, damage of stromal keratocytes also seems to be minimal.…”

Section: Discussionmentioning

confidence: 69%

Immunolocalization of proto-oncogene products in keratocytes after epithelial ablation, alkali burn and penetrating injury of the cornea in rats

Okada¹,

Saika²,

Shirai³

et al. 1998

Graefe's Archive for Clinical and Experimental Ophthalmology

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Section: Discussionmentioning

confidence: 69%

Immunolocalization of proto-oncogene products in keratocytes after epithelial ablation, alkali burn and penetrating injury of the cornea in rats

Okada¹,

Saika²,

Shirai³

et al. 1998

Graefe's Archive for Clinical and Experimental Ophthalmology

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“…Keratocyte loss, after removal of the corneal epithelium, can be prevented by a collagen shield alone or in combination with topical application of a corneal preservation medium. 37 Park and Tseng 38 reported that amniotic membrane precludes polymorphonuclear leukocyte infiltration and decreases lipid peroxidation and keratocyte death. Mohan et al 39 demonstrated that if mouse or rabbit corneas are enucleated and washed with a phosphatebuffered saline solution and the corneal epithelium is then scraped, the keratocytes undergo apoptosis.…”

Section: Commentmentioning

confidence: 99%

Confocal and Electron Microscopic Studies of Laser Subepithelial Keratomileusis(LASEK) in the White Leghorn Chick Eye

Lee¹

2002

Arch Ophthalmol

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To evaluate the effect of 20% alcohol on the white leghorn chick cornea and to determine the confocal and electron microscopic findings of laser subepithelial keratomileusis surgery in the white leghorn chick corneal model. Method: Laser subepithelial keratomileusis surgery was performed on chick corneas and the morphologic changes were examined by transmission electron microscopy. Chick corneas were exposed to 20% alcohol for 30 and 45 seconds or 1 and 2 minutes (5 chicks per group) to evaluate the effect on the corneal epithelium. Photorefractive keratectomy using either mechanical or 20% alcohol-assisted debridement (5 chicks per group) was also performed. Keratocyte and epithelial cell deaths were analyzed 4 hours after surgery using terminal deoxynucleotidyl transfermediated biotin-dexoyuridine 5-triphosphate nick-end labeling (TUNEL) staining and transmission electron microscopy. Results: Exposure of the corneal epithelium to 20% alcohol for 30 seconds or longer allowed reproducible separation of epithelial flaps in white leghorn chick eyes. Transmission electron microscopy immediately after alcohol treatment showed that exposure to 20% alcohol for 30 seconds or less had minimal adverse effects on the corneal epithelium. The TUNEL staining of corneas obtained 4 hours after surgery revealed TUNEL-positive cells in the central superficial stroma and more abundantly in the peripheral superficial stroma around the epithelial flap margin and in the epithelial flap itself, particularly in the basal epithelial layer. Transmission electron microscopy showed similar evidence of apoptosis in the epithelium and anterior stroma. Conclusions: The white leghorn chick eye seems to be a reasonable model for laser subepithelial keratomileusis surgery. Treatment with 20% alcohol for 30 seconds results in reproducible epithelial flap creation in the chick cornea and in relatively low levels of stromal and epithelial cell death after surgery.

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“…CCS can also promote stromal healing by limiting keratocyte loss and potentially reducing subepithelial corneal fibrosis (Willoughby and others 2002). In one study, rabbits underwent mechanical epithelial debridement and keratocyte loss was prevented using CCS soaked in 1% dextran, 2·5% chondroitin sulphate, vitamins, and precursors of adenosine triphosphate solution (Nassaralla and others 1995). CCSs support collagenases and polymorphonuclear leukocytes phagocytosis, thus reducing corneal stromal lysis and restoration of the corneal epithelium integrity (Schiff and others 1992).…”

Section: Discussionmentioning

confidence: 99%

Use of collagen shields for treatment of chronic bilateral corneal ulcers in a pet rabbit

Eshar

Wyre

Schoster

2011

Journal of Small Animal Practice

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A four-year-old, 1·3-kg entire male Holland Lop pet rabbit (Oryctolagus cuniculus) was presented with a two-day history of bilateral epiphora and blepharospasm. Fluorescein staining revealed bilateral medio-ventral corneal ulceration. Initial topical treatment included ophthalmic broad-spectrum antibiotics and artificial tear solutions. Over a six-month period, antibiotics were changed based on corneal culture and sensitivity test results, and periodic corneal debridement was performed. With little to no improvement, and recurrence of the previous clinical signs, surgical intervention was considered but withheld because of individual pet consideration and owner's reluctance. Hydrophilic contact lenses were placed for corneal support but failed and caused a severe ocular response. Corneal collagen shields were placed bilaterally in order to promote corneal healing. Recheck examination performed seven days after placement revealed complete resolution of the ulcer in one eye and major reduction of the corneal ulcer in the contra-lateral eye.

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Prevention of Keratocyte Loss After Corneal Deepithelialization in Rabbits

Cited by 11 publications

References 26 publications

Immunolocalization of proto-oncogene products in keratocytes after epithelial ablation, alkali burn and penetrating injury of the cornea in rats

Immunolocalization of proto-oncogene products in keratocytes after epithelial ablation, alkali burn and penetrating injury of the cornea in rats

Confocal and Electron Microscopic Studies of Laser Subepithelial Keratomileusis(LASEK) in the White Leghorn Chick Eye

Use of collagen shields for treatment of chronic bilateral corneal ulcers in a pet rabbit

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