Prevention of Hydrogen Peroxide‐Induced Red Blood Cells Lysis by Ilex paraguariensis Aqueous Extract: Participation of Phenolic and Xanthine Compounds

Abstract

The fresh leaves and stems of Ilex paraguariensis (Aquifoliaceae) are employed to prepare the commercial product used in North-eastern Argentina, Southern Brazil and Eastern Paraguay named yerba maté. The presence of polyphenols and xanthines, which present antioxidant activity, has been described in I. paraguariensis. In living organism, reactive oxygen species can cause tissue damage affecting erythrocyte membranes leading to hemolysis. The aim of this work was to evaluate the protective effect of an aqueous extract of I. paraguariensis (green leaves) on the hemolysis of red blood cells induced by hydrogen peroxide and to correlate this activity with the enzymatic activity related to hydrogen peroxide metabolism. The antioxidant activity of chlorogenic acid and caffeine was also analysed to evaluate their contribution to the activity of the crude extract. The extract as well as the isolated compounds protected red blood cells from hemolysis. This effect was related to a catalase-like activity. This study could contribute to the knowledge of the antioxidant activity of I. paraguariensis in view of the great quantities of yerba maté consumed by the population.

Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis

“…Produced by phagocytes and reactions catalyzed by xanthine oxidase and superoxide dismutase, hydrogen peroxide is highly reactive. It generates transition metal ion-dependent hydroxyl radicals that are highly potent at damaging cellular molecules [21,22]. From the results, it is possible that Chromolaena odorata contains compounds that scavenge hydrogen peroxide thus could protect tissues.…”

“…Hydrogen peroxide crosses biological membranes and slowly oxidizes a number of compounds [21]. Produced by phagocytes and reactions catalyzed by xanthine oxidase and superoxide dismutase, hydrogen peroxide is highly reactive.…”

Paracetamol and Hydrogen peroxide-induced tissue alterations are reduced by Costus afer extract

“…Hydrogen peroxide on the other hand is a potent reactive oxygen species that degrades heme, which leads to the release of redox active Fe ions that can initiate free radical production [27]. Hydrogen peroxide is highly diffusible along various cellular compartments in which it oxidizes various cellular targets [28]. Although produced by routine biochemical processes, it can cause cellular damage by generating the very potent hydroxyl radical via the Fenton reaction [28,29].…”

“…Hydrogen peroxide is highly diffusible along various cellular compartments in which it oxidizes various cellular targets [28]. Although produced by routine biochemical processes, it can cause cellular damage by generating the very potent hydroxyl radical via the Fenton reaction [28,29].…”

Phytochemistry and biology of Ilex pharmaceutical resources

“…Among the extracts prepared with different solvents, the 80% methanol extract showed the highest total polyphenol content (11.51/100 g) and antioxidant activity. Phenolic (e.g., chlorogenic acid) and xanthine compounds (e.g., caffeine) of I. paraguariensis are involved in the prevention of hydrogen peroxideinduced red blood cell lysis (Peralta et al, 2013). Phenolic (e.g., chlorogenic acid) and xanthine compounds (e.g., caffeine) of I. paraguariensis are involved in the prevention of hydrogen peroxideinduced red blood cell lysis (Peralta et al, 2013).…”