Prevention of fusion and leakage in freeze-dried liposomes by carbohydrates

Crowe, Lois M.; Womersley, Christopher; Crowe, John H.; Reid, David; Appel, Lori; Rudolph, Alan S.

doi:10.1016/0005-2736(86)90411-6

Cited by 220 publications

(73 citation statements)

References 32 publications

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“…Addition of a carbohydrate (trehalose) during freeze-drying prevents fusion and leakage of the vesicles. 35 …”

Section: Storage Of Liposomes: Freeze-dryingmentioning

confidence: 99%

Current trends in the production and biomedical applications of liposomes: a review

Uhumwangho¹,

Okor²

2009

Journal of Medicine and Biomedical Research

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A review of literature was carried out to determine methods of production of liposomes, their stability, biodistribution and their uses as drug delivery systems. The conventional method of preparing liposomes is basically for the multilamellar vesicles (MLVs). However, other methods are used to reduce the size of these MLVs to small unilamellar vesicles (SUVs) so as to increase their plasma lifetime and consequently increase the possibility of achieving greater tissue localisation. Some of these methods of size reduction are sonication and high pressure extrusion. Each of these methods has its own advantages and disadvantages. Large unilamellar vesicles (LUVs), on the other hand, are prepared mainly by detergent removal method and reverse phase extrusion technique. There are also improved pharmacokinetic properties with liposomal drugs compared to free drugs, though some formulation factors affect the release kinetics of the liposomal drugs. The review also shows that liposomes have a lot of biomedical applications and uses. They have been used in drug targeting, oral delivery of vaccines, insulins, peptides and some compounds, which are usually degraded in the gastrointestinal tract. It has also found application in topical therapy especially in the eye and lungs. Other areas of application are in cancer chemotherapy and treatment of human immunovirus (HIV) infection. The control of the stability of liposomes is an essential prerequisite for effective use as drug carriers. Leakage of the liposome is attributable mainly to differences in lamellar structure. For instance, MLVs are less prone to leakage than ULVs. The use of a combination of saturated phospholipid and cholesterol in the formulation of the liposomes has also been found to enhance stability with lower tendency to leakage.

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“…Addition of a carbohydrate (trehalose) during freeze-drying prevents fusion and leakage of the vesicles. 35 …”

Section: Storage Of Liposomes: Freeze-dryingmentioning

confidence: 99%

Current trends in the production and biomedical applications of liposomes: a review

Uhumwangho¹,

Okor²

2009

Journal of Medicine and Biomedical Research

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“…Bu amaçla laktoz, maltoz, sakkaroz ve trehaloz gıbi karbohidratlar kullanıl mıştır. Elektrolit içeren, bazı bileşiklerin ve gliserolün dondurmaeritme tekniğinde avantaj sağladığı, fakat liyofilizasyon sırasında kriyoprotektan (dondurma koruyucusu) olarak etkisiz olduğu anla şılmıştır (17)(18)(19).…”

Section: Lipozomların Stabilitelerinin Sağlanması Amacıyla Yapılan çAunclassified

“…Karbohidratlardan özellikle trehaloz'un füzyonu büyük ölçüde ön lediğini, diğerlerinin ise füzyon olayını kısmen önlediğini belirtmiş lerdir (18). Şekil 2'de liyofilizasyon işlemi sırasında kullanılan kriyoprotekranların lipozom yapısı üzerindeki etkisi görülmektedir.…”

Section: Lipozomların Stabilitelerinin Sağlanması Amacıyla Yapılan çAunclassified

Lipozomların Fiziksel ve Kimyasal Stabilitelerinin İncelenmesi : An Investigation of Physical and Chemical Stabilities on Liposomes

Bozkır¹,

Koçyiğit²

1995

Ankara Universitesi Eczacilik Fakultesi Dergisi

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ÖZETBu derlemede, ilaç taşıyıcı sistem olarak kullanılan lipozomların kimyasal ve fiziksel stabiliteleri incelenerek, stabilitelerini artırmak amacı ile yapılan çalışmalar özetlenmiştir.Anahtar kelimeler: Lipozom, Lipozomların stabilitesi, Lipo zomların fiziksel ve kimyasal stabilitesi. SUMMARYİn this review we have investigated the physical and chemical stabilities of liposomes as a drug delivery system and the studies to improve their stability were also summarized.

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“…( 1999). Freeze-dried liposomes were stabilized by trehalose (Crowe et al,1986). In the present encapsulation, therefore, trehalose would stabilize liposomes in a freeze-dried process in the DR method and result in high resistance of DR Iiposomes against the gastric environment.…”

mentioning

confidence: 94%

Preparation and Characterization of Liposomal Microencapsulated Poly-.GAMMA.-glutamic Acid for Prevention of Ca-Phosphate Precipitation under Intestinal Environment

Ishikawa

Shimoda

Matsumoto

2004

FSTR

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Prevention of fusion and leakage in freeze-dried liposomes by carbohydrates

Cited by 220 publications

References 32 publications

Current trends in the production and biomedical applications of liposomes: a review

Current trends in the production and biomedical applications of liposomes: a review

Lipozomların Fiziksel ve Kimyasal Stabilitelerinin İncelenmesi : An Investigation of Physical and Chemical Stabilities on Liposomes

Preparation and Characterization of Liposomal Microencapsulated Poly-.GAMMA.-glutamic Acid for Prevention of Ca-Phosphate Precipitation under Intestinal Environment

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