Prevention of diet-induced obesity in rats by oral application of collagen fragments

Raksha, Nataliia; Potalitsyn, Pavlo; Yurchenko, A.; Halenova, Tetyana; Savchuk, Olexiy; Li, Ostapchenko

doi:10.2298/abs170401027r

Cited by 11 publications

(6 citation statements)

References 46 publications

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“…These data are in congruence with our results of cytokines analysis, in which we reported that the application of LMMC in obesity model reduced the level of pro-inflammatory cytokines IL-1β and IL-12 and increased the level of anti-inflammatory cytokines IL-4 and IL-10, and partly restored superoxide dismutase and catalase activities in the serum ( 16 ). Similar results are reported in other studies which applied bioactive peptides: milk casein-derived tripeptide Val-Pro-Pro reduced macrophage infiltration into the adipose tissue (phenotype M1 macrophages (F4/80 + /CD11c + cells)) and suppressed inflammatory gene expression (MCP-1, a monocyte or macrophage chemotactic factor) in high-fat diet-induced murine adipose tissue inflammation ( 52 ).…”

Section: Resultssupporting

confidence: 92%

“…In our previous studies the low molecular mass collagen (LMMC) fragments derived from scales of the Antarctic wild marine fish demonstrated in diet-induced obesity model promising effects, namely: lowering of blood glucose, glycated hemoglobin and serum insulin concentrations. We hypothesized that observed effects might be used in reduction of mass and amelioration of obesityassociated comorbidities (16). Thus the main goal of our study was to analyze the effect of LMMC on visceral and subcutaneous white adipose tissue in high-calorie diet-induced obesity rats.…”

Section: Introductionmentioning

confidence: 99%

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Low-Molecular-Mass Fragments of Collagen Improve Parameters Related to Mass and Inflammation of the Adipose Tissue in the Obese Rat

Kalmukova

Raksha

Vovk

et al. 2023

Food Technol. Biotechnol. (Online)

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Research background. Despite clearly recognized links between increased body mass and increased risk for various pathological conditions, therapeutic options to treat obesity are still very limited. The aim of the present study was to explore the effect of low molecular mass collagen fragments obtained from the scales of Antarctic wild marine fish on rats' visceral and subcutaneous white adipose tissue in a high-calorie diet-induced obesity model. Experimental approach. The study was conducted on outbred rats, which were divided into 3 experimental groups: the first - control, consuming standard food (3.81 kcal/g), the second - obese group, consuming a high-calorie diet (5.35 kcal/g), and the third – obese group, consuming a high-calorie diet (5.35 kcal/g) with intragastric administration of low molecular mass collagen fragments (at a dose 1 g/kg of body mass during 6 weeks). Low molecular collagen fragments were obtained by procedure which included collagen extraction from fish scales and enzymatic hydrolysis with pepsin. Apart from H&E staining, fibrosis level was assessed by histochemical Van Gieson trichrome picrofucsin staining and analyses of mast cells were performed by toluidine blue O staining. Results and conclusions. Group treated by low molecular mass fragments of collagen exhibited decreased rate of mass gain, relative mass, area occupied by collagen fibers of both visceral and subcutaneous adipose tissue and reduction of cross-sectional area of both visceral and subcutaneous adipocytes. Treatment by low molecular mass fragments of collagen reduced infiltration of immune cells, number of mast cells and their redistribution back to the septa. This was as well accompanied by decreased number of the crown-like structures formed by immune cells, which are markers of chronic inflammation that accompany obesity. Novelty and scientific contribution. This is the first study which reported the anti-obesity effect of low molecular mass fragments produced as a result of controlled hydrolysis of collagen from the scales of Antarctic wild marine fish in vivo model. Another novelty of this work is observation that the tested collagen fragments not only reduce body mass, but as well improve morphological and inflammatory parameters (decrease in number of crown-like structures, immune cells infiltration, fibrosis and mast cells). Altogether, our work suggests that low molecular mass collagen fragments represent a promising candidate for amelioration of some comorbidities linked to obesity.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Low-Molecular-Mass Fragments of Collagen Improve Parameters Related to Mass and Inflammation of the Adipose Tissue in the Obese Rat

Kalmukova

Raksha

Vovk

et al. 2023

Food Technol. Biotechnol. (Online)

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“…In recent years, there has been growing interest in the use of bioactive peptides as alternative agents for the treatment of metabolic disorders ( 29 - 31 ). Our previous study revealed the weight-lowering effect of collagen fragments prepared from fish scales ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

Anti-obesity effect of collagen peptides obtained from Diplulmaris antarctica, a jellyfish of the Antarctic region

Raksha¹,

Halenova²,

Vovk³

et al. 2023

Croat Med J

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Aim To investigate the ability of collagen peptides derived from a jellyfish of the Antarctic region ( Diplulmaris antarctica ) to prevent the development of obesity in rats fed a high-calorie diet. Methods Collagen peptides were produced by pepsin hydrolysis of jellyfish-derived collagen. The purity of collagen and collagen peptides was confirmed by SDS-polyacrylamide gel electrophoresis. Rats were fed a high-calorie diet for ten weeks and were simultaneously orally administered collagen peptides (1 g per 1 kg of body weight every other day) starting from the fourth week. Body mass index (BMI), body weight gain, selected nutritional parameters, the key parameters associated with insulin resistance, and the level of oxidative stress markers were assessed. Results Compared with untreated obese rats, rats treated with hydrolyzed jellyfish collagen peptides had a decreased body weight gain and body mass index. They also had a decreased level of fasting blood glucose, glycated hemoglobin, insulin, lipid peroxidation products (conjugated dienes, Schiff bases), and oxidatively modified proteins, as well as a restored activity of superoxide dismutase.

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“…Fish collagen peptide from a water-hydrolyzed fraction decreased the accumulation of lipid and also decreased expression of C/EBPα, PPARγ, and adipocyte protein 2 (aP2) genes during the 3T3-L1 preadipocytes differentiation (109). Oral supplementation of collagen fragments decreased body fat and weight with an improved cytokine (110). Recent studies have shown anti-oxidant potential of food peptides.…”

Section: Marine Peptidesmentioning

confidence: 99%

Peptides and Peptidomimetics as Potential Antiobesity Agents: Overview of Current Status

Kumar

2019

Front. Nutr.

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There is a high occurrence of obesity worldwide without many new medications being approved for its treatment. Therefore, there is an urgent need to introduce new approaches for treating obesity. Bioactive peptides have been used to treat metabolic disorders- such as type-2 diabetes and obesity; while also possessing anti-oxidant, anti-inflammatory, anti-microbial, and anti-viral properties. However, the development of these peptides has taken backstage due to their size, reduced stability, poor delivery and bioavailability, fast rate of degradation etc. But with the emergence of newer techniques for multifunctional peptides, mimetics, peptide analogs, and aptamers, there is a sudden revival in this therapeutic field. An increased attention is required for development of the natural peptides from food and marine sources which can mimic the function of mediators involved in weight management to avoid obesity. Herein, the search for the structures of anti-obesity peptides was carried out in order to establish their potential for drug development in future. An extensive search for the current status of endogenous, food and marine peptides, with reference to novel and interesting experimental approaches based on peptidomimetics for controlling obesity, was performed. Apolipoprotein A-I (apoA-I), melanocortin-4 receptor (MC4R)-specific agonist, GLP-1 dual and triple agonists, neuropeptides and prolactin-releasing peptide mimetics were specifically examined for their anti-obesity role. Novel peptides, mimetics, and synthesis interventions are transpiring and might offer safer alternatives for otherwise scarcely available safe antiobesity drug. A deeper understanding of peptides and their chemistry through the use of peptide engineering can be useful to overcome the disadvantages and select best mimetics and analogs for treatment in future.

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Prevention of diet-induced obesity in rats by oral application of collagen fragments

Cited by 11 publications

References 46 publications

Low-Molecular-Mass Fragments of Collagen Improve Parameters Related to Mass and Inflammation of the Adipose Tissue in the Obese Rat

Low-Molecular-Mass Fragments of Collagen Improve Parameters Related to Mass and Inflammation of the Adipose Tissue in the Obese Rat

Anti-obesity effect of collagen peptides obtained from Diplulmaris antarctica, a jellyfish of the Antarctic region

Peptides and Peptidomimetics as Potential Antiobesity Agents: Overview of Current Status

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