Prevention of Colonization of the Urinary Tract of Mice with Escherichia coli by Blocking of Bacterial Adherence with Methyl  -D-Mannopyranoside

Aronson, Moshe; Medalia, Ora; Schori, L.; Mirelman, David; Sharon, Nathan; Ofek, Itzhak

doi:10.1093/infdis/139.3.329

Cited by 318 publications

(159 citation statements)

References 10 publications

Supporting

Mentioning

149

Contrasting

Unclassified

Order By: Relevance

“…Upon injection of the bacteria into the bladder, they adhere to the epithelial cells. The strain of E. coli that was used is strongly mannose sensitive (Aronson et al, 1979) and, therefore, can adhere to epithelial cells and to PMNs via mannose receptors. The PMNs however, appear only several hours after injection of bacteria (Cobb and Kaye, 1967), hence the initial inoculum must have already attached to the epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sequential Study of Bacterial Clearance in Experimental Cystitis

Gillon

Small²,

Medalia

et al. 1984

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

“…The procedure for producing experimental cystitis has been previously described in detail (Aronson et al, 1979), and will therefore be only briefly described. Escherichia coli strain 7343, an isolate from human pyelonephritis, was injected into the bladder of anaesthesised female ICR mice.…”

Section: Infectionmentioning

confidence: 99%

Sequential Study of Bacterial Clearance in Experimental Cystitis

Gillon

Small²,

Medalia

et al. 1984

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

“…Humans gained control over a 10 7 times smaller organism and the mortality from bacterial infections rapidly diminished and concomitantly also the interest to develop new antimicrobials. However, shortly after it was assumed that bacterial infections were under control, the first emergence of resistant strains evolved.…”

Section: Antibiotics and Resistance -The Microbiological Tw Insmentioning

confidence: 99%

In vivo Evaluation of FimH Antagonists – A Novel Class of Antimicrobials for the Treatment of Urinary Tract Infection

Abgottspon¹,

Ernst

2012

Chimia

View full text Add to dashboard Cite

The discovery of antimicrobials as β-lactam antibiotics or aminoglycosides revolutionized the treatment of infectious diseases. However, the extensive use rapidly created the problem of resistant pathogens, which are increasingly difficult to treat. FimH antagonists are a new class of antimicrobials, which target the bacterial adhesion to urothelial cells, a crucial first step in the establishment of urinary tract infections. Because of their different mode of action, FimH antagonists neither kill nor inhibit the growth of bacteria, they should have a reduced potential to generate resistant strains. This mini-review outlines the main problems associated with increasing development of antimicrobial resistance. Furthermore, it summarizes the currently available in vivo studies in mice for the treatment of urinary tract infections conducted with FimH antagonists.

show abstract

“…In the first study of its type, a mannose specific E. coli was injected into the urinary bladder of mice in the absence or presence of methyl cymannoside [28]. The sugar caused a significant decrease (by a factor of 3) in the extent of bacteriuria, compared to the control group ( fig.5).…”

Section: Initiation Of Infectionmentioning

confidence: 99%

Bacterial lectins, cell‐cell recognition and infectious disease

1987

View full text Add to dashboard Cite

Numerous bacterial strains produce surface lectins, commonly in the form of fimbriae that are filamentous assemblies of protein subunits. Among the best characterized of these are the type 1 (mannose specific) fimbrial lectins of Escherichia coli that consist almost exclusively of one class of subunit with a molecular mass of 17 kDa. They possess an extended combining site corresponding to a trisaccharide and preferentially bind carbohydrate units of oligomannose or hybrid type. Type 1 fimbriae also possess a hydrophobic region close to the carbohydrate-binding site, since aromatic a-mannosides inhibit strongly (up to lOOO-times more than methyl a-mannoside) the agglutination of yeasts by the bacteria and the adherence of the latter to pig ileal epithelial cells. The combining sites of type 1 fimbriae of the salmonellae and of other enteric bacteria are different from those of E. coli in that they are smaller and do not possess a hydrophobic region. The various bacterial surface lectins appear to function primarily in the initiation of infection by mediating bacterial adherence to epithelial cells, e.g. in the urinary and gastrointestinal tracts. The mannose specific lectins also act as recognition molecules in lectinophagocytosis (i.e. phagocytosis of the bacteria in the absence of opsonins) by mouse, rat and human peritoneal macrophages, and human polymorphonuclear leukocytes. Affinity chromatography of membrane lysates from human polymorphonuclear leukocytes on immobilized type 1 fimbrial lectin, using methyl u-mannoside as eluent, showed that glycoproteins with apparent molecular masses of 7&80, 100 and 150 kDa act as receptors for the bacteria. Inhibition experiments with monoclonal antibodies suggest that the glycoprotein bands of 100 and 150 kDa may be identical with the a and /l subunits of leukocyte complement receptors and adhesion glycoproteins involved in complement-mediated opsonophagocytosis. The systems described serve as a fine illustration for the biological role of lectin-carbohydrate interactions. Further studies of these systems will lead to a deeper understanding of the molecular basis of infectious diseases, and perhaps also to new approaches for their prevention.

show abstract

Prevention of Colonization of the Urinary Tract of Mice with Escherichia coli by Blocking of Bacterial Adherence with Methyl -D-Mannopyranoside

Cited by 318 publications

References 10 publications

Sequential Study of Bacterial Clearance in Experimental Cystitis

Sequential Study of Bacterial Clearance in Experimental Cystitis

In vivo Evaluation of FimH Antagonists – A Novel Class of Antimicrobials for the Treatment of Urinary Tract Infection

Bacterial lectins, cell‐cell recognition and infectious disease

Contact Info

Product

Resources

About