Prevention of Candida albicans Biofilm Formation by Covalently Bound Dimethylaminoethylmethacrylate and Polyethylenimine

Abstract

Candida albicans biofilms are a major cause of voice prosthesis deterioration in laryngectomized patients. The aim of this study was to produce a surface capable of inhibiting C. albicans biofilm formation. Dimethylaminoethylmethacrylate (DMAEMA) and polyethylenimine (PEI) moieties were covalently bound to the surface of polydimethylsiloxane (PDMS) or polymethylmethacrylate (PMMA) and subsequently quaternized. Physicochemical characterization of the grafted surfaces was carried out and their effect on C. albicans cell numbers was assessed using a modified Robbins device to grow the biofilms. Covalently bound quaternized polyDMAEMA (polyDMAEMAq) and PEI (PEIq) inhibited biofilm growth, with reductions up to 92%. Our approach may show promise for future application in medical devices such as catheters and prostheses.

Plant-Derived Decapeptide OSIP108 Interferes with Candida albicans Biofilm Formation without Affecting Cell Viability

“…Although the primary application of OSIP108 could be envisaged in systemic combination therapy, another potential application concerns its use as a coating of specific medical devices, such as dental implants, dentures, and voice prostheses, as C. albicans is the most prevalent fungus in the oral cavity (52) and is the most frequently isolated Candida spp. on voice prostheses in laryngectomized patients (53). Regarding to the latter, infection risk of these voice prostheses is very high (50 to 100%) compared to the risk with other implantable biomaterials (13).…”

Candida biofilm formation on voice prostheses

“…Because QAS coatings are non-toxic, clinical application could increase the useful lifetime of tracheoesophageal shunt prostheses by decreasing biofilm mass. In a recent study, De Prijck et al (2010) aimed to produce a surface capable of inhibiting C. albicans biofilm formation by dimethylaminoethylmethacrylate and polyethylenimine coating the surface of polydimethylsiloxane (silicone) or polymethylmethacrylate. Physico-chemical characterization of the grafted surfaces was carried out and their effect on C. albicans cell numbers was assessed using a modified Robbins device to grow the biofilms.…”

Silicone Rubber Modified with Methacrylic Acid to Host Antiseptic Drugs

“…Also, its capability to uptake drugs by soaking or impregnation is very limited due to its native inertness. Some approaches for SR modification with some materials (acrylamides, methacrylates, and diblock copolymers) using different grafting techniques have been developed to modulate cell/microorganism adhesion and to confer ability to load and release drugs . The micron‐size brushes or networks formed on the surface of the SR substrate should have adequate mesh size and chemical groups for the diffusion and binding of target drugs.…”

“…Some approaches for SR modification with some materials (acrylamides, methacrylates, and diblock copolymers) using different grafting techniques have been developed to modulate cell/microorganism adhesion and to confer ability to load and release drugs. [14,[16][17][18][19][20][21][22] The micron-size brushes or networks formed on the surface of the SR substrate should have adequate mesh size and chemical groups for the diffusion and binding of target drugs. Gamma radiation treatment is a simple and useful technique to functionalize the surface of polymeric biomaterials without altering their mechanical properties.…”