Prevention of bovine herpesvirus-1 transmission by the transfer of embryos disinfected with recombinant bovine trypsin

Bielański, A.; Algire, J.; Lalonde, A.; Garceac, A.

doi:10.1016/j.theriogenology.2013.08.012

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…The study demonstrated that the trypsin treatment was not able to eliminate the BoHV-1 of the embryos. However, Bielanski et al (2013) concluded that recombinant bovine trypsin (RBTr) was effective at the inactivation of BoHV-1 from ZP intact, in vivo produced embryos. The RBTr showed reduced infectivity of the BoHV-1 associated with the intact ZP, which prevented transmission of the disease to ET recipients and their offspring.…”

Section: Discussionmentioning

confidence: 99%

In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes

Gonçalves

Soares²,

Orlandi

et al. 2016

Arq. Inst. Biol.

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The aims of this study were to assess in vitro if bovine oocytes and oviductal epithelial cells from slaughterhouses for in vitro fertilization use may be infected with bovine herpesvirus 1; to analyze whether the treatment with trypsin according to the International Embryo Transfer Society guideline is efficient to inactivate the bovine herpesvirus 1; to morphologically study the virus-oocyte interaction through optical microscopy. In this study, Madin Darby Bovine Kidney (MDBK) cells that were co-cultured with oocytes matured in vitro and exposed to bovine herpesvirus 1 showed a cytopathic effect. The nested polymerase chain reaction for the supernatant was positive for the bovine herpesvirus 1, thus suggesting that the cytopathic effect observed in the MDBK monolayer was seen due to virus replication and not because of any culture toxicity. It was also observed cytopathic effect and positive nested polymerase chain reaction in MDBK cells co-cultured with in vitro maturated oocytes free of virus, but that were co-cultured in uterine epithelial cells pre-infected with bovine herpesvirus 1 and washed or not with trypsin, demonstrating an oocyte contamination by the virus. When trypsin-washing efficacy was evaluated, we could observe that the trypsin treatment was not able to eliminate the bovine herpesvirus 1 of the oocytes, and it was not observed any morphological difference in the infected oocytes.

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Section: Discussionmentioning

confidence: 99%

In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes

Gonçalves

Soares²,

Orlandi

et al. 2016

Arq. Inst. Biol.

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“…The effect of transferring LSDV infected embryos to recipient cows is not known. Bielanski et al reported that when unwashed in vivo produced embryos positive for BHV‐1 were transferred to seronegative cows, poor pregnancy rates and seroconversion resulted (Bielanski, Algire, Lalonde, & Garceac, ). In another experiment, the same authors found that if BVDV‐infected embryos were washed according to the IETS disinfection protocol prior to transfer, they did not trigger seroconversion in either the cows or their offspring (Bielanski, Algire, Lalonde, & Garceac, ).…”

Section: Discussionmentioning

confidence: 99%

Effect of using frozen–thawed bovine semen contaminated with lumpy skin disease virus on in vitro embryo production

Annandale

Smuts

Ebersohn

et al. 2019

Transbound Emerg Dis

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Summary Lumpy skin disease (LSD) is an important transboundary animal disease of cattle with significant economic impact because of the implications for international trade in live animals and animal products. LSD is caused by a Capripoxvirus, LSD virus (LSDV), and results in extensive hide and udder damage, fever and pneumonia. LSDV can be shed in semen of infected bulls for prolonged periods and transmitted venereally to cows at high doses. This study examined the effects of LSDV in frozen‐thawed semen on in vitro embryo production parameters, including viral status of media and resulting embryos. Bovine oocytes were harvested from abattoir‐collected ovaries and split into three experimental groups. After maturation, the oocytes were fertilized in vitro with frozen‐thawed semen spiked with a high (HD) or a lower (LD) dose of LSDV, or with LSDV‐free semen (control). Following day 7 and day 8 blastocyst evaluation, PCR and virus isolation were performed on all embryonic structures. After completing sufficient replicates to reach 1,000 inseminated oocytes, further in vitro fertilization (IVF) runs were performed to provide material for electron microscopy (EM) and embryo washing procedures. Overall, in vitro embryo yield was significantly reduced by the presence of LSDV in frozen‐thawed semen, irrespective of viral dose. When semen with a lower viral dose was used, significantly lower oocyte cleavage rates were observed. LSDV could be detected in fertilization media and all embryo structures, when higher doses of LSDV were present in the frozen‐thawed semen used for IVF. Electron microscopy demonstrated LSDV virions inside blastocysts. Following the International Embryo Transfer Society washing procedure resulted in embryos free of viral DNA; however, this may be attributable to a sampling dilution effect and should be interpreted with caution. Further research is required to better quantify the risk of LSDV transmission via assisted reproductive procedures.

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“…It has been shown to be effective for inactivating or removing BHV-1, BHV-4 and VSV. 24,25 On the other hand, pathogens such as H. somnus, U. diversum and Mycoplasma are not removed by trypsin treatment. 21 Washing IVP embryos is beneficial in reducing the number of infectious particles that can be present in the fluids used for IVM, IVF or IVC but it is not totally effective.…”

Section: Treatments To Remove Pathogens Attached To the Zpmentioning

confidence: 99%

“…Pathogens such BHV-1 become firmly attached to the ZP of IVP embryos and cannot effectively be removed by washing alone or with trypsin treatment. 25 Therefore, the recommended IETS protocol for removing certain pathogens from in vivo produced embryos may not be adequate in the case of IVP embryos.…”

Section: Treatments To Remove Pathogens Attached To the Zpmentioning

confidence: 99%

Embryo transfer, a potential risk in disease transmission

Escobar¹

2018

MOJAP

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Several studies have been performed to determine the disease transmission potential of early bovine embryos. Most of them agree that the Zona Pellucida (ZP) is a barrier that generally protects the embryo from infection but can also be a potential route for the transmission of pathogens. This review will initially focus on the characteristics of the ZP and the potential ways in which an embryo can transmit disease and the possible alternatives to reduce this hazard.

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Prevention of bovine herpesvirus-1 transmission by the transfer of embryos disinfected with recombinant bovine trypsin

Cited by 6 publications

References 24 publications

In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes

In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes

Effect of using frozen–thawed bovine semen contaminated with lumpy skin disease virus on in vitro embryo production

Embryo transfer, a potential risk in disease transmission

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