Prevention of bone loss by oil extract of garlic (Allium sativum Linn.) in an ovariectomized rat model of osteoporosis

Abstract

The effects of oil extract of garlic (Allium sativum Linn.) on different primary and secondary osteoporotic marker changes were tested in an ovariectomized rat model of osteoporosis. Experiments were performed on three different rat models: sham-operated control, ovariectomized and ovariectomized supplemented with garlic oil. In ovariectomized group, there has been a significant increase in different relative organ weights compared to sham-operated control, while the uterine weight was found to be decreased. Supplementation with oil extract of garlic could effectively reverse these changes. Also low bone densities that developed in the ovariectomized group were significantly recovered in the garlic oil supplemented group. In our study, the development of high rate of bone turnover and osteoporosis in the ovariectomized animals were confirmed by significant alteration of serum alkaline phosphatase activity, serum tartrate resistant acid phosphatase activity, urinary excretion of calcium, phosphate, hydroxyproline and urinary calcium to creatinine ratio, when compared with the sham-operated control group. Garlic oil extract supplementation, apart from its unique influence in lowering blood cholesterol, could also prevent ovariectomy-induced rise in all the above-mentioned marker changes. The results of this study emphasize that oil extract of garlic possibly has a positive role in suppressing ovariectomy-induced bone resorption.

Effect of Quercetin on Bone Mineral Status and Markers of Bone Turnover in Retinoic Acid-Induced Osteoporosis

“…These results revealed a low level of toxicity of 13cRA on bone and liver cells. These results are in agreement with fi ndings reported by other authors [Fahmy & Soliman, 2009;Mukherjee et al, 2004;Wei et al, 2007], who demonstrated that bone remodeling in rats is accelerated after ovariectomy-induced model of osteoporosis. These results are consistent with the data from Hotchkiss et al [2006] who showed that retinoids in large doses cause an increase of periosteal resorption in relation to periosteal extension or disposal of minerals, which ultimately leads to the reduction in the diameter of bone mineral loss.…”

Role of oil extract of garlic (Allium sativum Linn.) on intestinal transference of calcium and its possible correlation with preservation of skeletal health in an ovariectomized rat model of osteoporosis

“…It is considered as a dietary source of lignans, a type of phytoestrogen (Hernandez et al, 2004). Our previous study in an ovariectomized rat model of osteoporosis clearly established the importance of an oil extract of garlic as a possible nutraceutical which has therapeutic potential in preventing ovarian hormone insufficiencyinduced osteoporotic changes by influencing primary and secondary osteoporotic marker parameters and also bone density (Mukherjee et al, 2004).…”

Suppressive Effects of the Leaf of Terminalia catappa L. on Osteoclast Differentiation In Vitro and Bone Weight Loss In Vivo

“…For example, it has been reported that onion, garlic and parsley can inhibit bone resorption in ovariectomized rats (22,23). Quercetin from onion extract inhibits the differentiation of osteoclast progenitor into pre-osteoclasts and actin ring formation (24).…”