Prevention of bleomycin-induced pulmonary fibrosis after adenovirus-mediated transfer of the bacterial bleomycin resistance gene.

Tran, Phuong-Lan; Weinbach, Jérôme; Opolon, Paule; Linares-Cruz, Gustavo; Reynes, Jean-Paul; Grégoire, Anne; Kremer, Eric J.; Durand, Henri; Perricaudet, Michel

doi:10.1172/jci119203

Cited by 48 publications

(33 citation statements)

References 45 publications

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“…49 Protection of the rodent lung from bleomycininduced fibrosis has been reported using a transgene approach. 50 For irradiation protection, MnSOD overexpression is theoretically attractive as it does not push cells out of G 0 51 and has an advantage in this regard, especially for cells in the lung which may not be cycling during irradiation exposure.…”

Section: Discussionmentioning

confidence: 99%

Prevention of late effects of irradiation lung damage by manganese superoxide dismutase gene therapy

et al. 1998

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Section: Discussionmentioning

confidence: 99%

Prevention of late effects of irradiation lung damage by manganese superoxide dismutase gene therapy

et al. 1998

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“…For the quantitative morphological analysis of fibrotic changes in adult mouse lungs, a numerical fibrotic scale was used (31). Briefly, hematoxylin-eosin-stained lung sections were viewed under the microscope for identification of lesions.…”

Section: Methodsmentioning

confidence: 99%

Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice

Zhao

Shi

Wang

et al. 2002

American Journal of Physiology-Lung Cellular and Molecular Phys

367

287

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“…25 The plasmid pSK.CMVTIMP-3 was constructed by introducing the blunted fragment AgeI-NheI containing the cDNA of TIMP-3 from pBlast.HTLVTIMP-3 (Cayla, Toulouse, France) into the EcoRV sites of pSK.CMVpolyA, as described above. The construction of pSK.CMVnlslacZ was reported in Tran et al 25 Cell culture and in vitro gene transfer…”

Section: Construction Of Recombinant Plasmidsmentioning

confidence: 99%

“…Cells were detached, washed with PBS, suspended in serum-free culture medium, mixed in various ratios (25,50, 75 and 100% of transfected cells) with untransfected HuH7 cells, and plated onto 96-well plates. Cultures were incubated for 48, 72, and 96 hours.…”

Section: Assessment Of Bystander Effectmentioning

confidence: 99%

Gene therapy for hepatocellular carcinoma using non-viral vectors composed of bis guanidinium-tren-cholesterol and plasmids encoding the tissue inhibitors of metalloproteinases TIMP-2 and TIMP-3

et al. 2003

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Metalloproteinases (MMPs) and their natural inhibitors (TIMPs) contribute to the regulation of tumor microenvironment. Their expressions are deregulated in almost all human cancers. We report a novel approach to gene therapy of hepatocellular carcinoma (HCC), using repeated injections of DNA plasmids encoding the tissue inhibitors of metalloproteinases (TIMPs) TIMP-2 or TIMP-3, and a novel competent formulation of gene transfer based on nontoxic cationic cholesterol derivatives. The new gene delivery system was efficient in demonstrating the antitumor efficiency of TIMP-2 or TIMP-3 in inhibiting tumor growth of human HuH7 HCC cells xenografted into nude mice. We show, for the first time, an in vivo effect of TIMP-3 in delaying HCC tumor growth. No treatment-related toxicity was noted. An inhibition of angiogenesis and tumor necrosis accompanied the inhibitory effects of TIMP-2 or TIMP-3 on tumor expansion and invasion. We also report a bystander effect produced by transfected HuH7 tumor cells mixed with untransfected cells in 1:1 ratio in culture that resulted in killing 98% of cells within 96 h. In addition, the soluble forms of TIMP-2 and TIMP-3 expressed by transfected cells exerted a cytotoxic effect on untransfected HuH7 cell cultures. Taken together, these results demonstrate the potential efficacy of repeated treatment of secreted TIMP-2 and TIMP-3 for the design of nonviral gene therapy for hepatocarcinoma.

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Prevention of bleomycin-induced pulmonary fibrosis after adenovirus-mediated transfer of the bacterial bleomycin resistance gene.

Abstract: Abstract

Cited by 48 publications

References 45 publications

Prevention of late effects of irradiation lung damage by manganese superoxide dismutase gene therapy

Prevention of late effects of irradiation lung damage by manganese superoxide dismutase gene therapy

Smad3 deficiency attenuates bleomycin-induced pulmonary fibrosis in mice

Gene therapy for hepatocellular carcinoma using non-viral vectors composed of bis guanidinium-tren-cholesterol and plasmids encoding the tissue inhibitors of metalloproteinases TIMP-2 and TIMP-3

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