Prevention of biofilm formation on titanium surfaces modified with conjugated molecules comprised of antimicrobial and titanium-binding peptides

Yoshinari, Masao; Kato, Tsuyoshi; Matsuzaka, Kenichi; Hayakawa, Tohru; Shiba, Kiyotaka

doi:10.1080/08927010903216572

Cited by 100 publications

(76 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hauser-Gerspach et al reported that release of F ions from resin composites decreased bacterial adhesion 25) . In earlier studies, we demonstrated a number of mechanisms capable of inhibiting biofilm accumulation on a Ti surface: coating with alumina for inhibition of initial attachment 21) , application of F + -implantation 33) and immobilization of anti-microbial peptides 42) . Such surface modification may also prove to be effective on TZP surfaces.…”

Section: Discussionmentioning

confidence: 99%

In vitro adherence of periodontopathic bacteria to zirconia and titanium surfaces

et al. 2013

Self Cite

View full text Add to dashboard Cite

Tetragonal zirconia polycrystals (TZP) has drawn attention as a potential alternative to titanium (Ti) in dental implant treatment, as it minimizes both allergic reactions and esthetic problems. It is also important for dental implants to maintain plaque-free surfaces to prevent peri-implantitis. The purpose of this study was to investigate in vitro adherence of periodontopathic bacteria to TZP comparing with Ti. Periodontopathic bacteria were cultured on polished discs of two kinds of TZP, and Ti as a control. After incubation, the numbers of adherent bacteria were estimated. No significant differences among specimens were observed in the initial attachment, although a decrease was observed in initial attachment to saliva-coated specimens. In the bacterial colonization, no significant differences were recognized among specimens. The adherence of the periodontopathic bacteria on TZP was similar to that on Ti. These results suggest that a strategy is required for inhibition of the bacterial adherence to TZP.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro adherence of periodontopathic bacteria to zirconia and titanium surfaces

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…33,[36][37][38] Yoshinari et al reported that the modification of Ti surface with titaniumbinding peptides (minTBP-1) and lactoferricin showed a reduction in the bioactivity of P. gingivalis. 39 Although the antibacterial mechanism of LF on gram-positive pathogens is still not well understood, the interaction between LF and the cell membrane of gram-positive bacteria has been reported. Leitch et al reported that LF binds to lipoteichoic acid (LTA), which is a component of nearly all grampositive membranes and increases the permeability of lysozyme causing the release of LTA from the cell surface.…”

Section: B Inhibition Of S Gordonii Adhesion and The Bactericidal Ementioning

confidence: 99%

Inhibition of initial bacterial adhesion on titanium surfaces by lactoferrin coating

et al. 2014

View full text Add to dashboard Cite

Because dental implant abutments are located at transmucosal sites, their surface should inhibit bacterial accumulation to prevent peri-implantitis. The authors examined the effects of human lactoferrin (LF), an antibacterial protein present in saliva, as an antibacterial coating on the titanium surface and evaluated its effects before and after mucin-containing artificial saliva (AS) incubation. In the control group, titanium disks were soaked in distilled water, whereas in the LF group, titanium disks were soaked in LF solution to coat the disks. In the control-AS and LF-AS groups, half of the control and LF disks were incubated with AS. To confirm LF adsorption, the fluorescence intensity of fluorescein isothiocyanate-labeled LF was measured. The LF and LF-AS groups showed significantly higher intensity than the control and control-AS groups (P < 0.01). There was no significant difference between the LF and LF-AS groups (P > 0.05). The amount of adhered Streptococcus gordonii significantly increased by incubation with AS (P < 0.01) and significantly decreased by adsorption of LF (P < 0.01). There was no interaction between the two factors, LF adsorption and AS incubation (P = 0.561). These results suggest that the adsorbed LF inhibited bacterial adhesion following AS incubation. According to qualitative LIVE/DEAD analysis, viable bacteria appeared to be decreased in the presence of LF and SEM observation indicated that altered morphologies increased in LF and LF-AS groups. These results suggest that the adsorbed LF remained on the titanium surface after incubation with AS, and the remaining LF inhibited bacterial adhesion and exhibited bactericidal effects. Therefore, the adsorption of LF on the abutment material appears to be effective in preventing peri-implantitis.

show abstract

“…In vitro antimicrobial studies have found that a GL13K peptide coating is bactericidal and inhibits biofilm growth for pathogens related to peri-implantitis, such as P. gingivalis, S. gordonii and P. aeruginosa under static growth conditions 142,155) . Furthermore, AMP surfaces displayed antimicrobial activity under dynamic growth conditions against S. gordonii 156) and under static growth conditions against Streptococcus mutans, Staphylococcus epidermidis and Escherichia coli 159) .…”

Section: Antimicrobial Peptide Surfacesmentioning

confidence: 99%

Antimicrobial dental implant functionalization strategies —A systematic review

Grischke

Eberhard

Stiesch

2016

Dental Materials Journal

View full text Add to dashboard Cite

Prevention of biofilm formation on titanium surfaces modified with conjugated molecules comprised of antimicrobial and titanium-binding peptides

Abstract: Specific binding of antimicrobial peptides to titanium surfaces may serve to prevent biofilm formation, leading to a reduction in peri-implantitis. This study evaluated the binding behavior of conjugated molecules consisting of antimicrobial and hexapeptidic titanium-binding peptides

Cited by 100 publications

References 39 publications

In vitro adherence of periodontopathic bacteria to zirconia and titanium surfaces

In vitro adherence of periodontopathic bacteria to zirconia and titanium surfaces

Inhibition of initial bacterial adhesion on titanium surfaces by lactoferrin coating

Antimicrobial dental implant functionalization strategies —A systematic review

Contact Info

Product

Resources

About