Prevention of biofilm formation by dairy products and N-Acetylcysteine on voice prostheses in an artificial throat

Schwandt, Leonora Q.; Weissenbruch, Ranny van; Stokroos, I.; Mei, Henny C. van der; Busscher, Henk J.; Albers, F. W. J.

doi:10.1080/00016480410022516

Cited by 40 publications

(28 citation statements)

References 15 publications

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“…Sonication was capable of detaching more bacteria than the NAC and scraping methods, thus confirming the usefulness of this method reported in clinical studies [4,12,23,[28][29][30]. Some studies [2,10,16,26,33] have shown that NAC alone or in combination with other compounds has strong activity against bacterial biofilm, and the mechanism involving the disruption of biofilm may induce the susceptibility of bacteria during antibiotic Fig. 9A-H Confocal laser scanning microscopy shows the formation and removal of biofilms on prosthetic materials for S aureus on PE discs (Stains: bacteria were stained green by Syto-9, the biofilm matrix was stained red by FilmTracer TM SYPRO Ruby Matrix Stain; original magnification, x 3000).…”

Section: Discussionsupporting

confidence: 64%

“…Sonication of explanted implants has notably improved sensitivity of microbiologic diagnosis of PJIs by dislodging bacteria from biofilm adhered to prosthetic surfaces, as documented in numerous studies [1,4,12,13,23,[28][29][30]. Since NAC exerts activity against bacterial biofilms [2,10,16,26,33], we presumed NAC and DTT could be used to remove bacteria from a prosthetic biofilm. We asked the following questions: (1) Do DTT and NAC remove bacteria from biofilm formed on prosthetic materials?…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Does Dithiothreitol Improve Bacterial Detection from Infected Prostheses? A Pilot Study

Drago

Romanò

Mattina

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Does Dithiothreitol Improve Bacterial Detection from Infected Prostheses? A Pilot Study

Drago

Romanò

Mattina

et al. 2012

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

“…Pérez-Giraldo et al (1997) studied the influence of various concentrations of N-acetylcysteine on the formation of biofilms of different strains of Staphylococcus epidermidis and found a dose-related decrease in biofilm and slime formation. Schwandt et al (2004) inoculated Groningen button and Provox2 VPs with a mixture of bacteria and yeasts. On Groningen button VPs Nacetylcysteine reduced the amount of bacteria and yeasts to 2.4 % (P,0.01) and 55 % (P,0.15) of the control value, respectively.…”

Section: Prophylactic Treatment Of Silicone Rubber Voice Prosthesesmentioning

confidence: 99%

Candida biofilm formation on voice prostheses

Talpaert

Balfour

Stevens

et al. 2015

Journal of Medical Microbiology

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“…These properties may contribute to the prevention and disruption of biofilm around different polymeric and metallic surfaces (9,(19)(20)(21). Not only does NAC diminish the formation of biofilm by common pathogens (19,22,23), it also possesses some in vitro intrinsic antimicrobial activity against both Gram-positive and Gram-negative bacteria (24). Taking into consideration the therapeutic safety record and the antibiofilm ability of NAC combined with antimicrobial activity of a broad-spectrum antibiotic, impregnation of intravascular catheters with this unique combination can be a promising approach for reducing catheter colonization and potential subsequent catheter-associated infection.…”

mentioning

confidence: 99%

In Vitro Activity and Durability of a Combination of an Antibiofilm and an Antibiotic against Vascular Catheter Colonization

Mansouri

Hull

Stager

et al. 2013

Antimicrob Agents Chemother

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bCatheter-associated infections can cause severe complications and even death. Effective antimicrobial modification of catheters that can prevent device colonization has the potential of preventing clinical infection. We studied in vitro the antimicrobial activities of central venous catheters impregnated with N-acetylcysteine (NAC), an antibiofilm agent, and a broad-spectrum antibiotic against a range of important clinical pathogens. NAC-levofloxacin-impregnated (NACLEV) catheters were also evaluated for their antiadherence activity. NACLEV catheters produced the most active and durable antimicrobial effect against both Grampositive and Gram-negative isolates and significantly reduced colonization (P < 0.0001) by all tested pathogens compared to control catheters. These in vitro results suggest that this antimicrobial combination can potentially be used to combat catheter colonization and catheter-associated infection. Intravascular catheters are essential in managing critically ill patients. However, vascular catheter-associated infections could result in dire consequences leading to excessive morbidity and mortality, longer hospital stays, and higher health care costs, with an average treatment cost of $25,000 per episode (1-5).Catheter-associated infections are generally initiated by microbial colonization of the catheter surface and formation of a superficial biofilm layer (6). Cell surface proteins and polysaccharide production by bacterial cells reportedly contribute to the formation of biofilm that can then protect pathogens by impeding both the penetration of antibiotics and the function of phagocytic immune cells, thus hindering the ability to combat colonizing pathogens (7-9).Impregnation or coating of catheters with antimicrobial agents has commonly been used to prevent bacterial colonization of vascular catheters (8). However, some existing antimicrobial-treated catheters designed to prevent catheter colonization may have partial clinical efficacy, particularly against drug-resistant pathogens, and limited durability of antimicrobial activity (8, 10-13) partly due to their inability to control biofilm formation and combat biofilm-nested microorganisms, which can have MICs of up to 1,000 times higher than their MICs against their free-floating planktonic counterparts (8,(14)(15)(16).N-acetylcysteine (NAC), a commonly used inhalation mucolytic therapy for chronic bronchitis and an FDA-approved intravenous injection for the treatment of acetaminophen toxicity (17), has favorable pharmacokinetics when used in hemodialysis patients (18). NAC also adversely affects bacterial growth and polysaccharide production and disrupts disulfide bonds in mucus, reducing the viscosity of secretions. These properties may contribute to the prevention and disruption of biofilm around different polymeric and metallic surfaces (9,(19)(20)(21). Not only does NAC diminish the formation of biofilm by common pathogens (19,22,23), it also possesses some in vitro intrinsic antimicrobial activity against both Gram-positive and Gr...

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Prevention of biofilm formation by dairy products and N-Acetylcysteine on voice prostheses in an artificial throat

Cited by 40 publications

References 15 publications

Does Dithiothreitol Improve Bacterial Detection from Infected Prostheses? A Pilot Study

Does Dithiothreitol Improve Bacterial Detection from Infected Prostheses? A Pilot Study

Candida biofilm formation on voice prostheses

In Vitro Activity and Durability of a Combination of an Antibiofilm and an Antibiotic against Vascular Catheter Colonization

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