Prevention of Alloxan Induced Diabetes Mellitus in Rats by Vitamin a Dietary Supplementation

“…[4,5] Oxidant-free radicals have been implicated in the pathogenesis of IDDM. [1,6,7] In experimental animals, injection and subsequent metabolism of 2, 4, 5, 6-tetraoxypyrimidine (alloxan) induces specific DNA fragmentation in pancreatic islets and cell damage has been attributed to the production of toxic free radicals. [1,8] Alloxan-treated animals are widely used as models for IDDM studies.…”

Section: Introductionmentioning

confidence: 99%

“…[1,6,7] In experimental animals, injection and subsequent metabolism of 2, 4, 5, 6-tetraoxypyrimidine (alloxan) induces specific DNA fragmentation in pancreatic islets and cell damage has been attributed to the production of toxic free radicals. [1,8] Alloxan-treated animals are widely used as models for IDDM studies. [1,3,[9][10][11][12][13] The reason for the high sensitivity of β-cells to alloxan is not clear, although there are speculations on the connection between alloxan sensitivity and the incidence of IDDM.…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Hypoglycemic properties of ethanolic extracts of Gongronema latifolium, Aloe perryi, Viscum album and Allium sativum administered to alloxan-induced diabetic albino rats (Rattus norvegicus)

Ibegbulem¹,

Chikezie²

2013

Phcog Commn

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Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia and glycosuria produced by an absolute or relative insulin insufficiency (Insulin-Dependent Diabetes Mellitus (IDDM)) or insulin resistance by peripheral cells (Non-Insulin-Dependent Diabetes Mellitus (NIDDM)), or both. [1] The disease presents other metabolic and anatomic distortions and disturbances, including retinopathy, neuropathy, nephropathy, hyperlipidemia, ABSTRACT: Background: Plants offer a wide range of natural compounds of medicinal values to humans and domestic animals. Objective: The ethanolic extracts of Gongronema latifolium, Aloe perryi, Viscum album (leaves) and Allium sativum (bulb) were investigated for their phytochemical/biochemical constituents and hypoglycemic properties. Materials and Methods: Hypoglycemia was induced in rats by a single dose (140 mg/kg) of intra-peritoneal injection of alloxan monohydrate in citrate buffer (pH 4.5). Suspensions of the ethanolic extracts were administered by intraperitoneal injection at doses of 2 mg/kg every 16 h for 54 h. Collection of blood samples for estimation of fasting blood glucose (FBG) was carried out at regular time intervals of 0, 16, 32, 48 and 54 h, using the glucose oxidase method. Results: Phytochemical and biochemical screening showed the presence of saponins, tannins, flavonoids, proteins and carbohydrates in the four plant tissues under investigation. A. sativum and G. latifolium also tested positive for the presence of alkaloids. The capacities of the four ethanolic extracts to reduce FBG concentrations in treated rats at the 54 h were in the order: A. perryi > G. latifolium > A. sativum > V. album. Comparatively, at t = 16 h, FBG concentration of V. album treated rats was not significantly different (p > 0.05) from those of G. latifolium treated group. Likewise, FBG concentration of rats treated with V. album extract did not show a significant difference (p > 0.05) compared to the group administered with extract of A. sativum. Conclusion: The four plant extracts used in the present study exhibited approximately the same capacity to act as hypoglycemic agents in the treated rats and correlate with the therapeutic capacity of the standard drug, glimepiride.

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“…It is the most common endocrine disorder which in turn damage many of the body's systems, in particular the blood vessels and nerves [1][2] .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2011

IJPSR

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Diabetes is a chronic metabolic disorder characterized by altered carbohydrates, fat and protein metabolism, and an increased risk of vascular complications. Swiss albino mice weighing 20-25g and albino rats (Wistar strain) weighing 170-190g of either sex were used for the studies. Mice used for toxicity and rats for anti-diabetic study. The aqueous roots extract of Murva was found to be safe at a dose of 2000mg/kg; hence a dose of 800mg/kg was used for anti-diabetic study. Diabetes was induced by alloxan monohydrate 150mg/kg i.p. After three days of injection of alloxan, diabetes was confirmed by monitoring its blood glucose level. Animals with blood glucose level more than 200mg/dl were taken for studies. Glibenclamide 20mg/kg i.p was taken as std. drug. The test possessed significant antidiabetic activity (p<0.001) when compared to positive and negative control. Hence the roots extract of Murva may be value in patients with diabetes mellitus.

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Prevention of Alloxan Induced Diabetes Mellitus in Rats by Vitamin a Dietary Supplementation

Cited by 19 publications

References 16 publications

Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract.

Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract.

Hypoglycemic properties of ethanolic extracts of Gongronema latifolium, Aloe perryi, Viscum album and Allium sativum administered to alloxan-induced diabetic albino rats (Rattus norvegicus)

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