Prevention of adrenalectomy changes in the anterior pituitary of the rat by sodium chloride administration

Koneff, Alexei A.; Holmes, R. O.; Reese, Jeff

doi:10.1002/ar.1090790302

Cited by 8 publications

(1 citation statement)

References 27 publications

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“…These cells were chro-mophobes in respect to aniline blue staining but were interpreted by the authors to be basophils in the last stages of granular depletion and to represent an attempt to compensate for the degenerative changes seen in many basophils. In a later paper, Koneff, Holmes, and Reese (57) reported that maintenance of the adrenalectomized rats on 1 per cent sodium chloride in drinking water prevented this degranulation due to excessive activity of basophil cells.…”

Section: Discussionmentioning

confidence: 95%

Identification of the Adrenocorticotrophin-Producing Cells in the Rat Hypophysis by Autoradiography

Siperstein

1963

The Journal of Cell Biology

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The relative rates of protein (hormone) synthesis and secretion by the various cell types in the anterior hypophysis of the rat have bcen studied by means of autoradiography. Normal and adrenalectomized male rats wcre injected with tritiated glycine and their hypophyses removed and fixed at 20, 40, and 90 minutes and 15 hours after injection. Autoradiograms of the hypophysial sections were prepared and autoradiographic grains were counted in thc film overlying the cytoplasm of individual cells. With the aid of this method, a unique cell type was identificd in the hypophyscs of adrenalcctomizcd rats. This ccll is morphologically distinct from "gonadcctomy cells," "thyroidectomy cells," and from previously described normal cell types, and is thcrcfore designated as the "adrenalectomy cell." Among the 7 cell types differentiated in this study, the "adrenalectomy cell" had the highest tritium content and, in addition, at the time intcrvals studied this cell had the fastcst rate of appearance and disappcarance of protein tritium. This autoradiographic evidence of rapid protein (or polypeptide) turnover following adrcnalectomy indicates that the "adrenalectomy cell" is the site of adrenocorticotrophin production in the adrcnalectomized rat. Further autoradiographic and cytological evidence is presented which suggests that the "adrenalectomy cells" may be derived from chromophobes, and that a portion of the "large chromophobcs" as defined in this study may be the sitc of adrenocorticotrophin production in the normal rat.

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Section: Discussionmentioning

confidence: 95%