Prevention of Adhesion and Surface Growth of Orthopedic Implant Microbial Infection by Surface Modification Using Antibiotics and Irradiated Hydroxyapatite

Farrag, Hala A.; El-Hendawy, Hoda H.; Tablawy, S. Y. El; Nora, F. H.

doi:10.1007/s12633-017-9571-8

Cited by 2 publications

(4 citation statements)

References 28 publications

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“…The nanobacterial isolates were tested for their ability to produce biofilm in the tube, according to Christensen et al 13 and Farrag et al 14 …”

Section: Methodsmentioning

confidence: 99%

“…Adherence was measured using spectrophotometric methods described by Christensen et al 15 and Farrag et al 14 …”

Section: Methodsmentioning

confidence: 99%

“…A combination treatment between the potent antibiotics and an aqueous extract of the plant on biofilm production by certain strong biofilm‐producing nanobacterial strains was carried out according to Christensen et al 15 and Farrag et al 14 …”

Section: Methodsmentioning

confidence: 99%

“…The nanobacterial isolates were tested for their ability to produce biofilm in the tube, according to Christensen et al 13 and Farrag et al 14 2.6 | Quantitative determination of biofilm production by nanobacterial isolates using ELISA method Adherence was measured using spectrophotometric methods described by Christensen et al 15 and Farrag et al 14 The isolates were classified based on ODs as OD ≤ 0.120 weak adherent, 0.120 < OD ≤ 0.240 moderate adherent, and OD > 0.24 strong adherent, according to Mathur et al 16 and Vasanthi et al 17…”

Section: Qualitative Determination Of Biofilm Production By Nanobacte...mentioning

confidence: 99%

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Synergistic effect of doxycycline and aqueous extract of irradiated khella on structure of nanobacteria isolated from kidney stones: In vitro and in vivo studies

Fayez,

Ibrahim,

Farrag

et al. 2023

Cell Biochemistry & Function

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Kidney stones have been associated with an increased risk of chronic kidney diseases, end‐stage renal failure. This study is devoted to isolate nanobacteria from patients with active urolithiasis and investigate the in vitro and in vivo antinanobacterial activity of some antibiotics alone or in combination with extracts of irradiated herbs from certain medicinal plants. Nanobacteria were detected using scanning (SEM) and transmission (TEM) electron microscopy, protein electrophoresis (SDS‐PAGE) and DNA profile. The antimicrobial susceptibility of some biofilm‐producing nanobacterial isolates was evaluated. The effect of medicinal plant extracts on growth was tested. A combination treatment between the most potent extracts and antibiotics was tested on biofilm production, protein profile, release of 260 nm absorbing material, protein content, and ultrastructure of the strongest biofilm producers. In vivo study of nanobacteria and its treatment by the most potent agents was evaluated on male rats. Renal function was measured in serum; histological examination and oxidative stress parameters were determined in kidney tissues. Results showed that streptomycin, trimethoprim/sulfamethoxazole, doxycycline, and water extracts of irradiated khella at 6 kGy had antinanobacterial activity. Meanwhile, the synergistic effect of the aqueous extract of irradiated Khella and doxycycline showed higher inhibition activity on microbial growth and biofilm production. They affected dramatically the strength of its cell membrane and subsequently its ultrastructure. Moreover, these results are confirmed by ameliorations in renal function and histological alterations. It could be concluded that the combination of DO and an aqueous extract of irradiated khella has an antinephrotoxic effect against nanobacteria‐induced renal toxicity.

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“…The nanobacterial isolates were tested for their ability to produce biofilm in the tube, according to Christensen et al 13 and Farrag et al 14 …”

Section: Methodsmentioning

confidence: 99%

“…Adherence was measured using spectrophotometric methods described by Christensen et al 15 and Farrag et al 14 …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Qualitative Determination Of Biofilm Production By Nanobacte...mentioning

confidence: 99%

See 2 more Smart Citations

Synergistic effect of doxycycline and aqueous extract of irradiated khella on structure of nanobacteria isolated from kidney stones: In vitro and in vivo studies

Fayez,

Ibrahim,

Farrag

et al. 2023

Cell Biochemistry & Function

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Influence of the Biological Medium on the Properties of Magnesium Doped Hydroxyapatite Composite Coatings

et al. 2023

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In this paper, the stability of magnesium-doped hydroxyapatite/chitosan (MHC) suspension obtained with the sol-gel approach was evaluated using nondestructive ultrasound measurements. The MHC coatings obtained by the spin-coating technique were characterized before and after immersion for 7 and 14 days, respectively, in Dulbecco’s modified eagle medium (DMEM) by scanning electron microscopy, equipped with an EDAX detector. Also, the functional groups present on the MHC coatings surface were analyzed with the aid of attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy. The surface microstructure was evaluated using two commentary techniques, namely atomic force microscopy (AFM) and metallographic microscopy (MM). The influence of immersion in DMEM on the biological properties was studied with in vitro studies using primary osteoblast and HCT-8 cell lines. Our results revealed that both surface morphology and chemical composition of the MHC coatings allow rapid development of a new apatite layer on their surface after immersion in DMEM. Preliminary in vitro biological studies underlined the noncytotoxic effect of the studied samples on the proliferation of primary osteoblast and HCT-8 cell lines, which makes them a promising candidate for applications in fields such as orthopedics or dentistry. The antifungal assay of the MHC coatings was assessed using Candida albicans ATCC 10231 and their results showed a good inhibitory effect. The coatings made on the basis of the MHC composite could contribute to increasing the degree of success of implants by decreasing the risk of infections and postoperative inflammation.

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Prevention of Adhesion and Surface Growth of Orthopedic Implant Microbial Infection by Surface Modification Using Antibiotics and Irradiated Hydroxyapatite

Cited by 2 publications

References 28 publications

Synergistic effect of doxycycline and aqueous extract of irradiated khella on structure of nanobacteria isolated from kidney stones: In vitro and in vivo studies

Synergistic effect of doxycycline and aqueous extract of irradiated khella on structure of nanobacteria isolated from kidney stones: In vitro and in vivo studies

Influence of the Biological Medium on the Properties of Magnesium Doped Hydroxyapatite Composite Coatings

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