Prevention by Phytochrome of Photodelay in Chlorophyll Accumulation

Oelze‐Karow, H.; Rösch, H.; Mohr, Hans

doi:10.1111/j.1751-1097.1983.tb04519.x

Cited by 19 publications

(6 citation statements)

References 17 publications

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“…In the case of the OY-YG lesion, the 60% decrease in CPII*/CPII peripheral LHCII complexes and the corresponding decrease in 8 nm PFs particles suggests (a) that many granal PSII centers may be disassociated from this 'excitation pool' (there are not enough peripheral LHCII complexes to go around), and (b) that these PSII centers, nevertheless, retain their granal localization (evidenced by the unchanged EFs/EFu particle content ratio) and possibly their coupling to the PQ pool. In a companion paper to this one (18) (26), suggesting a possible site for the OY-YG lesion. In the case that OY-YG is truly a photoregulatory dysfunction, it would be an ideal system for the study (Table II), we have estimated a rough order of Chl b-association and resulting stability whereby CP29 has approximately twice the affinity for Chl b as does LHCI, which in turn is greater than that of the peripheral (mobile) LHCII.…”

Section: Light-sensitive Chlorophyll B-deficient Mutant Of Maize Resultsmentioning

confidence: 89%

Hierarchical Response of Light Harvesting Chlorophyll-Proteins in a Light-Sensitive Chlorophyll b-Deficient Mutant of Maize

Greene¹,

Allred²,

Morishige³

et al. 1988

Plant Physiol.

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The light-sensitive chlorophyll b (Chl b)-deficient oil yellow-yellow green (OY-YG) mutant of maize (Zea mays) grown under conditions of high light exhibits differential reductions in the accumulation of the three major Chl b-containing antenna complexes and characteristic changes in thylakoid architecture. When observed by freeze-fracture electron microscopy, the most notable changes in the OY-YG thylakoid structure are: (a) a major reduction in the number of 8 nanometer particles of the protoplasmic fracture face of stacked membrane regions (PFs) paralleled by a 60% reduction in the chlorophyll-proteins (CP) associated with the peripheral light harvesting complex (LHCII) for photosystem II (PSII) and which give rise to the LHCII oligomer/monomer (CPH*/CPII) bands on mildly dissociated green gels; (b) a sizable decrease in the proportion of 11 to 13 nanometer particles of the protoplasmic fracture face of unstacked membrane regions (PFu) that parallels the loss of light harvesting complex I (LHCI) antennae from photosystem I (PSI) centers and a 40% reduction of the band containing CP1 and LHCI (CPI*) on mildly dissociating green gels; (c) an unchanged or slightly increased average size of particles of the exoplasmic fracture face of stacked (or appressed) membrane regions (EFs) along with a relative increase in CP29, the postulated bound LHC of PSII, and of CP47 and CP43, PSII core antenna complexes. This latter result sets the OY-YG mutant apart from all other Chl b-deficient mutants studied to date, all of which possess EFs particles that are substantially reduced in size. Based on these findings, we postulate that the bound LHCII associated with EFs particles consists mostly of CP29 chlorophyll proteins and very little, if any, CPII*/CPII chlorophyll proteins. Indeed, the CPII*/CPII chlorophyll proteins may be exclusively associated with the 'peripheral' LHCII units that give rise to 8 nanometer PF particles. The differential effect of the Chl b deficiency on the accumulation of the three main antenna complexes (CPII*/CPII>CPI*>CP29) suggests, furthermore, that there is a hierarchy among Chl b-binding proteins, and that this hierarchy might be an integral part of long-term photoregulation mediating Chl b partitioning in the chloroplast.It has long been known that plants can adjust their photosynthetic apparatus in response to changes in their immediate light

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Section: Light-sensitive Chlorophyll B-deficient Mutant Of Maize Resultsmentioning

confidence: 89%

Hierarchical Response of Light Harvesting Chlorophyll-Proteins in a Light-Sensitive Chlorophyll b-Deficient Mutant of Maize

Greene¹,

Allred²,

Morishige³

et al. 1988

Plant Physiol.

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“…The red-light wavelength dependence and far-red light reversibility of this effect indicate that phytochrome is the photoreceptor that mediates the response (Virgin 1961;De Greef and Caubergs 1972;Oelze-Karow et al 1983;Briggs et al 1988). Other studies have indicated that induction of the synthesis of both Chl precursors (Masoner and Kasemir 1975;Huang et al 1989) and Chl-binding apoproteins (Bennett et al 1984;M6singer et al 1988;Wehmeyer et al 1990) is under phytochrome control.…”

Section: Discussionmentioning

confidence: 94%

“…Phytochrome and cytokinins have been implicated in the greening process (Virgin 1961;De Greefand Caubergs 1972;Oelze-Karow et al 1983;Briggs et al 1988). Phytochrome phototransformation induces the synthesis of the chlorophyll precursor, 6-aminolevulinic acid (Masoner and Kasemir 1975;Huang et al 1989) and is also involved in induction of the synthesis of mRNA for Chl-binding proteins (Bennett et al 1984;M6singer et al 1988;Wehmeyer et al 1990).…”

Section: Introductionmentioning

confidence: 98%

External calcium requirements for light induction of chlorophyll accumulation and its enhancement by red light and cytokinin pretreatments in excised etiolated cucumber cotyledons

Reiss

Beale

1995

Planta

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Abstract. Excised etiolated cucumber (Cucumis sativus L.) cotyledons that were depleted of external Ca 2 + by equilibration with a Ca 2 + buffer, which maintained the free Ca 2+ concentration at 10 -8 M, failed to accumulate chlorophyll upon a 2-h exposure to white light. Increasing amounts of chlorophyll accumulation occurred at increasing external Ca 2+ concentrations within the range of 10-7-10-3 M. Preillumination with red light or pretreatment with benzyladenine, which enhanced the rate of light-induced chlorophyll accumulation in control cotyledons, did not overcome the block to light-induced chlorophyll accumulation caused by the depletion of external Ca 2 +. Etiolated cotyledons that were treated with the Ca 2 § ionophore, A23187, and then equilibrated with 10-5M Ca 2+, accumulated significantly more chlorophyll during exposure to light than did untreated cotyledons. The enhancing effect of A23187 was approximately equal to that caused by red-light pretreatment. Etiolated cotyledons that were exposed to the Ca 2+-channel-blocking agent, Nd 3 + (neodymium), in the presence of 10-s M Ca 2 +, did not exhibit an enhancement of chlorophyll accumulation by red-light pretreatment, although they accumulated control levels of chlorophyll upon exposure to light and showed control levels of enhancement of chlorophyll accumulation by cytokinin pretreatment. Conversely, etiolated cotyledons that were equilibrated with 10-SM Ca 2+ in the presence of nifedipine, a blocker of some Ca 2+ channels, did not exhibit an enhancement of chlorophyll accumulation by cytokinin pretreatment, although they accumulated control levels of chlorophyll upon exposure to light and showed control levels of enhancement of chlorophyll accumulation by red-light pretreatment. These results indicate that external Ca 2+ is required for chlorophyll Abbreviations: A23187=antibiotic 23187 calcium ionophore; Chl = chlorophyll; nifedipine = 1,4-dihydro-2,6-dimethyl-4-(2'-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethyl ester Correspondence to: C. Reiss; FAX: 1 (607) 255 5407; E-mail: hcrl@cornell.edu accumulation by excised etiolated cucumber cotyledons during the first 2 h of light exposure, and that an influx of external Ca 2 + is required for the enhancing effect of redlight and cytokinin. The differential abilities of Nd 3 § and nifedipine to block the effects of red-light and cytokinin pretreatments suggests that enhancement of chlorophyll accumulation by red-light and cytokinin may involve different classes of Ca 2 § channels.

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“…Pf~ has a stabilizing action on Chl in light [35] and Chl might thus be protected from photobleaching. It was repeatedly observed that under higher irradiances Chl is lost due to photobleaching [28,34,14,45] ; R especially favours photodestruction of Chl [15] as well as decreasing the capacity of P Chl Ode) biosynthesis at later stages of development [42].…”

Section: Discussionmentioning

confidence: 99%

Ribulose bisphosphate carboxylase capacity and chlorophyll content in developing seedlings of Chenopodium rubrum L. growing under light of different qualities and fluence rates

et al. 1985

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In order to evaluate the aclimation of Chenopodium seedlings to different quantum fluence rates of R and BL, kinetics of Rubisco capacity, Chl content and chloroplast structure were studied. Under monochromatic light photoreceptors are stimulated selectively and their influence on biosynthetis capacities during chloroplast development can be studied.R irradiations saturate Rubisco capacity even at the lowest quantum fluence rates applied, whereas Chl a+b synthesis depends strongly upon fluence rate of R. Under BL irradiations, both Rubisco capacity and Chl content are fluence rate dependent. R irradiations favour Chl b synthesis relative to Chl a, whereas under BL Chl a content is high relative to Chl b. Under R irradiation Pfr is the main photoreceptor involved in regulation of Rubisco capacity whereas under BL a specific BL absorbing photoreceptor may control the response. From the fluence rate dependency under BL irradiations it is concluded that the blue region of the day light spectrum may be the sensor for monitoring fluence rate and causing the characteristic changes in shade and high/low WL adaptation with respect to Rubisco levels in Chenopodium.

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Prevention by Phytochrome of Photodelay in Chlorophyll Accumulation

Cited by 19 publications

References 17 publications

Hierarchical Response of Light Harvesting Chlorophyll-Proteins in a Light-Sensitive Chlorophyll b-Deficient Mutant of Maize

Hierarchical Response of Light Harvesting Chlorophyll-Proteins in a Light-Sensitive Chlorophyll b-Deficient Mutant of Maize

External calcium requirements for light induction of chlorophyll accumulation and its enhancement by red light and cytokinin pretreatments in excised etiolated cucumber cotyledons

Ribulose bisphosphate carboxylase capacity and chlorophyll content in developing seedlings of Chenopodium rubrum L. growing under light of different qualities and fluence rates

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