Prevention and treatment of inflammatory arthritis with traditional Chinese medicine: Underlying mechanisms based on cell and molecular targets

Li, Wenhao; Lei, Yu; Li, Wenming; Ge, Gaoran; Ma, Yong; Xiao, Long; Qiao, Yusen; Huang, Wei; Huang, Wenli; Wei, Minggang; Wang, Zhirong; Bai, Jiaxiang; Geng, Dechun

doi:10.1016/j.arr.2023.101981

Cited by 21 publications

(8 citation statements)

References 260 publications

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“…There is increasing evidence that OA is a dynamic process that results from joint injury and re-modelling imbalance, with the major cause being an excess of pro-in ammatory substances that trigger chondrocyte apoptosis and matrix deterioration [26] . With a comprehensive and synergistic approach, herbal medicine has drawn interest in the cure of OA by preserving the body's equilibrium [27] . By integrating clinical observations, network pharmacological techniques, and in-vitro studies, we have methodically evaluated the mechanism of action of Xinfeng capsule in osteoarthritis in our study.…”

Section: Discussionmentioning

confidence: 99%

Chinese herbal compound Xinfeng capsule inhibits cartilage extracellular matrix degradation and inflammation in osteoarthritis by modulating circ_0032131 and the miR-502-5p/TRAF2 axis

zhouqiao,

Liu,

et al. 2023

Preprint

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Objective We intended to explore the anti-inflammatory and chondroprotective effects of Xinfeng capsule (XFC) in osteoarthritis (OA), perhaps through the regulation of hsa_circ_0032131 and miR-502-5p/TRAF2 axis. Materials and methods In total, 30 patients with OA and healthy subjects were recruited. To detect markers of cartilage metabolism and inflammation, peripheral blood mononuclear cells (PBMCs) were taken out. Subsequently, network pharmacology was employed to forecast OA-related targets and pathways for XFC therapy. To investigate the function of upregulated hsa_circ_0032131 in model cells.A series of functional experiments revealed the relationship between hsa_circ_0032131 and miR-502-5p/TRAF2 axis. To further determine whether XFC potentially treats OA through the interaction between circ_0032131 and miR-502-5p/TRAF2 axis. CKK-8 assay and flow cytometry were performed to detect cell proliferation and apoptotic processes in XFC-treated cells. Some conventional experimental methods were used to detect the expression levels of inflammatory factors, extracellular matrix and others. In addition, rescue experiments verified that XFC blocked the effects of hsa_circ_0032131 overexpression on extracellular matrix, inflammation and cell viability. Results Clinical observations indicated that the expression of hsa_circ_0032131 in PBMCs of OA patients was significantly elevated, and there was a correlation with clinical immuno-inflammatory factors and inflammatory indicators. Network pharmacology verified that the chief active ingredients of XFC exerted their roles mainly in the regulation of inflammation (IL1A, IL1B, IL4), extracellular matrix metabolism (MMP13, COL2A1), and tumour necrosis factor (TNF, TRAF2). In vitro experiments revealed that knockdown of circ_0032131 inhibited apoptosis, inflammatory and ECM degradation in PBMCs-stimulated chondrocytes. Circ_0032131 was verified to be a sponge of miR-502-5p by targeting, and TRAF2 was a direct target of miR-502-5p. By regulating circ_0032131 and miR-502-5p/TRAF2 axis, XFC prevented PBMCs-stimulated chondrocytes from responding to inflammation and ECM degradation. Conclusion The XFC suppressed inflammatory response and extracellular matrix metabolism in OA by regulating circ_0032131 and miR-502-5p/TRAF2 axis.

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Section: Discussionmentioning

confidence: 99%

Chinese herbal compound Xinfeng capsule inhibits cartilage extracellular matrix degradation and inflammation in osteoarthritis by modulating circ_0032131 and the miR-502-5p/TRAF2 axis

zhouqiao,

Liu,

et al. 2023

Preprint

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“…The unique properties of natural products, such as multitarget effects based on multiple compounds, are thought to be well adapted to the requirements of DMOADs in drug discovery ( Yang et al, 2017 ; Luo et al, 2020 ; Jo et al, 2021 ; 2023b ; Panossian, 2023 ; Ren et al, 2023 ). Especially, East Asian herbal medicines (EAHM) have a long history of use by many people in East Asia, and in recent years, considerable scientific evidence has been compiled regarding their mechanisms for improving clinical arthritis symptoms and inhibiting inflammatory pathology, which can be considered the best data source for anti-OA drug development ( Li and Zhang, 2020 ; Wang et al, 2020 ; 2022 ; Jo et al, 2022 ; Liang et al, 2022 ; Zhang et al, 2022 ; Li et al, 2023 ). Recent EAHM studies have shown that several traditionally used herbs can inhibit the complex pathology of OA, including generalized inflammation, subchondral bone destruction, cartilage loss, and synovial defects, and this inhibition is based on their multi-component pharmacology.…”

Section: Introductionmentioning

confidence: 99%

Network analysis, in vivo, and in vitro experiments identified the mechanisms by which Piper longum L. [Piperaceae] alleviates cartilage destruction, joint inflammation, and arthritic pain

Jo,

Baek,

Kim

et al. 2024

Front. Pharmacol.

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Osteoarthritis (OA) is characterized by irreversible joint destruction, pain, and dysfunction. Piper longum L. [Piperaceae] (PL) is an East Asian herbal medicine with reported anti-inflammatory, analgesic, antioxidant, anti-stress, and anti-osteoporotic effects. This study aimed to evaluate the efficacy of PL in inhibiting pain and progressive joint destruction in OA based on its anti-inflammatory activity, and to explore its potential mechanisms using in vivo and in vitro models of OA. We predicted the potential hub targets and signaling pathways of PL through network analysis and molecular docking. Network analysis results showed that the possible hub targets of PL against OA were F2R, F3, MMP1, MMP2, MMP9, and PTGS2. The molecular docking results predicted strong binding affinities for the core compounds in PL: piperlongumine, piperlonguminine, and piperine. In vitro experiments showed that PL inhibited the expression of LPS-induced pro-inflammatory factors, such as F2R, F3, IL-1β, IL-6, IL-17A, MMP-1, MMP-2, MMP-3, MMP-9, MMP-13, NOS2, PTGS2, PGE2, and TNF-β. These mechanisms and effects were dose-dependent in vivo models. Furthermore, PL inhibited cartilage degradation in an OA-induced rat model. Thus, this study demonstrated that multiple components of PL may inhibit the multilayered pathology of OA by acting on multiple targets and pathways. These findings highlight the potential of PL as a disease-modifying OA drug candidate, which warrants further investigation.

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“… 2 People with OA may experience joint pain, stiffness, swelling, deformity, and disability. 3 The etiology of OA is still unknown, and its risk factors include joint degeneration and heredity, age, obesity, cumulative strain, joint trauma, and deformity. 4 With the aging of the global population, OA has imposed a substantial economic burden on patients and society.…”

Section: Introductionmentioning

confidence: 99%

Bibliometric Analysis of Research on Traditional Chinese Exercise and Osteoarthritis

Zeng,

Liu,

et al. 2024

JPR

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Background: Osteoarthritis (OA) is a common disease in geriatric rehabilitation medicine caused by the progressive destruction of articular cartilage. Traditional Chinese exercise (TCE) is an important component of traditional sports in China and aims to stretch the musculoskeletal tract and relieve joint pain. Bibliometrics can help researchers find suitable partners and understand the research hotspots and trends in a certain field. However, there is still a lack of bibliometric analysis in the field of TCE and OA. Methods: All the literature was obtained from the Web of Science Core Collection database. The last search was performed on July 28, 2023. The bibliometric indicators, such as publications, citations, and H-index, were recorded. Bibliometrix and CiteSpace were used for visualization analysis. In addition, randomized controlled trials were included to summarize the exercise prescription of TCE for OA. Results: A total of 170 articles were included. The field of OA with TCE had great development potential and was in the rising period. The countries, institutions, and authors with the most publications were the United States, Tufts Medical Center, and Harvey WF, respectively. The most popular journal was Osteoarthritis and Cartilage. The recent burst keywords in this field were mainly "hip", "pilot", and "risk". Tai Chi was the most studied TCE with the most detailed content of exercise prescription, followed by Baduanjin and Wuqinxi. Conclusion:Our study provides a basis for researchers in this field to choose appropriate partner and academic journals. Moreover, pain, muscle strength, and quality of life management of elderly OA patients are research hotspots in this field. The intervention of hip OA risk through TCE is expected to become a research direction for emerging teams. The TCE prescription we summarized can better provide researchers with more treatment details.

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Prevention and treatment of inflammatory arthritis with traditional Chinese medicine: Underlying mechanisms based on cell and molecular targets

Cited by 21 publications

References 260 publications

Chinese herbal compound Xinfeng capsule inhibits cartilage extracellular matrix degradation and inflammation in osteoarthritis by modulating circ_0032131 and the miR-502-5p/TRAF2 axis

Chinese herbal compound Xinfeng capsule inhibits cartilage extracellular matrix degradation and inflammation in osteoarthritis by modulating circ_0032131 and the miR-502-5p/TRAF2 axis

Network analysis, in vivo, and in vitro experiments identified the mechanisms by which Piper longum L. [Piperaceae] alleviates cartilage destruction, joint inflammation, and arthritic pain

Bibliometric Analysis of Research on Traditional Chinese Exercise and Osteoarthritis

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