Preventing contamination of PCR‐based multiplex assays including the use of a dedicated biosafety cabinet

Bouam, Amar; Vincent, Jean-Jacques; Drancourt, Michel; Raoult, Didier; Lévy, Pierre-Yves

doi:10.1111/lam.13375

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…In this study, confirmed FP detections could possibly be attributed to contamination occurring during the workflow process (e.g., while pipetting and transferring samples) ( 19 – 21 ). Thus, preventive measures, such as cleaning the laboratory bench after every sample, are essential.…”

Section: Discussionsupporting

confidence: 65%

Evaluation of the QIAstat-Dx Meningitis/Encephalitis Panel, a multiplex PCR platform for the detection of community-acquired meningoencephalitis

Sundelin,

Bialas,

de Diego

et al. 2023

J Clin Microbiol

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Rapid identification of the causative pathogens of central nervous system infections is essential for providing appropriate management and improving patient outcomes. The performance of QIAstat-Dx Meningitis/Encephalitis (ME) Panel—a multiplex PCR testing platform—in detecting pathogens implicated in meningitis and/or encephalitis was evaluated using BioFire FilmArray ME Panel as a comparator method. This multicenter study analyzed 585 retrospective residual cerebrospinal fluid specimens and 367 contrived specimens. The QIAstat-Dx ME Panel showed positive percent agreement (PPA) values of 100% for Neisseria meningitidis , Streptococcus agalactiae , Escherichia coli K1, Listeria monocytogenes , and Cryptococcus gattii / neoformans on clinical samples compared to the BioFire FilmArray ME Panel. The PPA values observed for Haemophilus influenzae and Streptococcus pneumoniae were 80% and 88.24%, respectively. Negative percent agreement (NPA) values were >99.0% for each of the six bacterial targets and one fungal target tested with clinical samples. One viral target, herpes simplex virus 1, exhibited a PPA value of 100.0%, while the remaining viral targets—human parechovirus, herpes simplex virus 2, human herpes virus 6, and varicella zoster virus—were >90.0%, with the exception of enterovirus, which had a PPA value of 77.8%. The QIAstat-Dx ME Panel detected five true-positive and four true-negative cases compared to BioFire FilmArray ME Panel. The NPA values for all viral pathogens were >99.0%. Overall, the QIAstat-Dx ME Panel showed comparable performance to the BioFire FilmArray ME Panel as a rapid diagnostic tool for community-acquired meningitis and encephalitis.

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Section: Discussionsupporting

confidence: 65%

Evaluation of the QIAstat-Dx Meningitis/Encephalitis Panel, a multiplex PCR platform for the detection of community-acquired meningoencephalitis

Sundelin,

Bialas,

de Diego

et al. 2023

J Clin Microbiol

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“…Indeed, suspensions of Hi and HSV-1 had been prepared near the POC SPCR assays preparation site shortly before. These results are a reminder of the vulnerability of POC SPCR assays to contaminations, due to their implementation in a not dedicated area to molecular biology [ 20 , 21 ]. A reserved cabinet was used for the following assays.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Performance Characteristics of a New POC Multiplex PCR Assay for the Diagnosis of Viral and Bacterial Neuromeningeal Infections

Bars

Madany²,

Lamoureux

et al. 2023

Diagnostics

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Point-of-care syndromic PCR (POC SPCR) assays are useful tools for the rapid detection of the most common causative agents of community-acquired infections responsible for meningitis and encephalitis infections. We evaluated the performance characteristics of the new QIAstat-Dx® Meningitis/Encephalitis panel (QS) compared to the laboratory reference methods and the POC SPCR Biofire® FilmArray® Meningitis Encephalitis Panel (FA). Viral (Enterovirus, Parechovirus, HSV-1, HSV-2, HHV-6, VZV) and bacterial (E. coli K1, H. influenzae, L. monocytogenes, encapsulated N. meningitidis, M. pneumoniae, S. agalactiae, S. pneumoniae, S. pyogenes) pathogens were suspended at low concentrations and tested with the POC SPCR systems. The reproducibility, analytical specificity, carryover contamination, interferences and clinical samples were evaluated. All samples tested positive with both QS and FA except for those containing the lowest concentrations of Enterovirus-D68-B3, Echovirus-30 and S. agalactiae which were only detected by FA. In terms of analytical specificity, we observed 3 false positive results out of 48 QS tests versus 1 out of 37 FA tests. For the other studied criteria, both QS and FA performed as expected. Our results suggest that the performance characteristics of QS are close to those of FA. A prospective multicenter study would be useful to complete the performances evaluation of QS.

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Implementation, Optimization, and Stewardship of Advanced Molecular Testing for Infectious Diseases

Azimi,

Dumm

2024

Advances in Molecular Pathology

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Preventing contamination of PCR‐based multiplex assays including the use of a dedicated biosafety cabinet

Cited by 6 publications

References 21 publications

Evaluation of the QIAstat-Dx Meningitis/Encephalitis Panel, a multiplex PCR platform for the detection of community-acquired meningoencephalitis

Evaluation of the QIAstat-Dx Meningitis/Encephalitis Panel, a multiplex PCR platform for the detection of community-acquired meningoencephalitis

Evaluation of the Performance Characteristics of a New POC Multiplex PCR Assay for the Diagnosis of Viral and Bacterial Neuromeningeal Infections

Implementation, Optimization, and Stewardship of Advanced Molecular Testing for Infectious Diseases

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