Preventing adulteration and fraud in botanical ingredients in the international marketplace: the ABC-AHP-NCNPR Botanical Adulterants Prevention Program

Blumenthal, Mark; Gafner, Stefan; Foster, Susan D.; Cardellina, JH; Khan, IA; Upton, Roy

doi:10.1055/s-0039-3399662

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“…But in the context of food safety where adulteration, substitution, and the presence of allergens may be important considerations, there are meaningful trade-offs to consider. Species-specific assays are neither designed to detect contaminant [17] allergens nor pathogen content in foods, which is of particular concern when considering that economically motivated adulteration in dietary supplements has been routinely documented [18,19] and is a very important food safety consideration.…”

Section: Hplc-uv Metabarcoding and Genome Skims Of Botanical Dietarymentioning

confidence: 99%

HPLC-UV, Metabarcoding and Genome Skims of Botanical Dietary Supplements: A Case Study in Echinacea

et al. 2021

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The use of DNA-based methods to authenticate botanical dietary supplements has been vigorously debated for a variety of reasons. More comparisons of DNA-based and chemical methods are needed, and concordant evaluation of orthogonal approaches on the same products will provide data to better understand the strengths and weaknesses of both approaches. The overall application of DNA-based methods is already firmly integrated into a wide array of continually modernizing stand alone and complementary authentication protocols. Recently, the use of full-length chloroplast genome sequences provided enhanced discriminatory capacity for closely related species of Echinacea compared to traditional DNA barcoding approaches (matK and rbcL). Here, two next-generation sequencing approaches were used: (1) genome skimming and (2) PCR amplicon (metabarcoding). The two genetic approaches were then combined with HPLC-UV to evaluate 20 commercially available dietary supplements of Echinacea representing “finished” products. The trade-offs involved in different DNA approaches were discussed, with a focus on how DNA methods support existing, accepted chemical methods. In most of the products (19/20), HPLC-UV suggested the presence of Echinacea spp. While metabarcoding was not useful with this genus and instead only resolved 7 products to the family level, genome skimming was able to resolve to species (9) or genus (1) with the 10/20 products where it was successful. Additional ingredients that HPLC-UV was unable to identify were also found in four products along with the relative sequence proportion of the constituents. Additionally, genome skimming was able to identify one product that was a different Echinacea species entirely.

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Section: Hplc-uv Metabarcoding and Genome Skims Of Botanical Dietarymentioning

confidence: 99%

HPLC-UV, Metabarcoding and Genome Skims of Botanical Dietary Supplements: A Case Study in Echinacea

et al. 2021

View full text Add to dashboard Cite

show abstract

Preventing adulteration and fraud in botanical ingredients in the international marketplace: the ABC-AHP-NCNPR Botanical Adulterants Prevention Program

Cited by 1 publication

References 0 publications

HPLC-UV, Metabarcoding and Genome Skims of Botanical Dietary Supplements: A Case Study in Echinacea

HPLC-UV, Metabarcoding and Genome Skims of Botanical Dietary Supplements: A Case Study in Echinacea

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