Preventative effects of Lactobacillus plantarum YS-3 on oxazolone-induced BALB/c colitis in mice

Xia, Feng; Zhang, Jing; Qian, Yu; Yi, Ruokun; Sun, Peng; Mu, Jianfei; Song, Jia‐Le

doi:10.1007/s13765-018-0359-3

Cited by 7 publications

(7 citation statements)

References 18 publications

(21 reference statements)

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“…Many pieces of literature have reported that probiotics for intestinal flora can improve intestinal barrier function by regulating inflammatory factors. [23][24][25] In the present study, LA-XY27 treatment could effectively reduce the levels of many proinflammatory cytokines, including IFN-γ, IL-1 β, IL-6, TNF-α, and IL-12, in the serum, and increase the levels of anti-inflammatory cytokine IL-10. This result was further verified by gene expression in the colon of mice with colitis.…”

Section: Discussionsupporting

confidence: 50%

Preventive Effect of Lactobacillus acidophilus XY27 on DSS-Induced Ulcerative Colitis in Mice

Hu¹,

Wang²,

Xiang³

et al. 2020

DDDT

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Introduction Lactobacillus acidophilus is widely used as probiotic supplement in functional foods due to its beneficial regulatory effects on host, such as immune regulation, anti-inflammatory, and antioxidant activities. Aim This study aimed to determine the preventive effect of Lactobacillus acidophilus XY27 ( L. acidophilus XY27) on colitis induced by dextran sodium. Methods The mice were randomly divided into five groups. Except for the control group, the other four groups were induced for ulcerative colitis (UC) with dextran sodium sulfate (DSS), and three groups in DSS-groups were treated with L. acidophilus XY27, L. bulgaricus , and salicylazosulfapyridine. The weight change, DAI score, colon length, and length to weight ratio were tested. The oxidation index and the levels of inflammatory cytokines in the serum were measured. Subsequently, the gene expression levels of inflammatory factors in the colon tissue were determined by the Real-Time quantitative polymerase chain reaction (qRT-PCR) method. Results The results showed that the mice in the L. acidophilus XY27 group performed better in terms of weight, DAI score, colon length, and length to weight ratio or colonic pathological sections compared with the DSS-induced group. Further, the levels of tumor necrosis factor α (TNF-α), Interleukin-6 (IL-6), Interleukin-12 (IL-12) and Interleukin-1β (IL-1β), malondialdehyde (MDA) content, and myeloperoxidase activity in the serum of UC mice treated with L. acidophilus XY27 significantly decreased, while the levels of Interferon-γ (IFN-γ), Interleukin-10 (IL-10), Catalase (CAT), and total superoxide dismutase (SOD) significantly increased. The gene expression levels of Ets-like transcription factor-1 (EIK-1), IL-12, IL-1β, Cyclooxygenase 2 (COX-2), TNF-α, Escherichia coli, Lipopolysaccharide (LPS), and p100 in the colon significantly decreased while those of tight junction protein 1 (ZO-1), nuclear factor kappa B (NF-kB), p53, and NF-kappa-B inhibitor alpha (IκB-α) increased in the L. acidophilus XY27 group. Conclusion The results of the experiment suggested that L. acidophilus XY27 prevented colitis and alleviated symptoms in mice with DSS-induced UC, and also repaired the intestinal barrier function.

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Section: Discussionsupporting

confidence: 50%

Preventive Effect of Lactobacillus acidophilus XY27 on DSS-Induced Ulcerative Colitis in Mice

Hu¹,

Wang²,

Xiang³

et al. 2020

DDDT

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“…SCF is an endogenous ligand for the c‐kit. The growth of ICC is decreased following the dysregulation of the SCF/c‐Kit pathway, thus exacerbating UC (Feng et al., 2018). IL‐8 exhibits chemotactic and inflammatory activities, and CXCR is highly specific for IL‐8.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of Lactobacillus plantarum YS3 on dextran sulfate sodium‐induced colitis in C57BL/6J mice

Fan

Long

et al. 2021

J. Food Biochem.

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The protective effect of Lactobacillus plantarum YS3 (LP-YS3) on ulcerative colitis (UC) was assessed using a mouse model of dextran sodium sulfate (DSS)-induced colitis. Different concentrations of LP-YS4 were administered to the experimental mice by daily gavage. Several inflammatory and biochemical indices, such as interleukin-2 (IL-2), interleukin-10 (IL-10), interleukin-6 (IL-6), interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNFα), glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), and nitric oxide (NO), were examined in mouse serum and colon tissue. The mRNA and protein expression levels of c-Kit, CXC chemokine receptor type 2 (CXCR2), interleukin-8 (IL-8), and stem cell factor (SCF) in mouse colon tissue were assessed using Western blot and quantitative polymerase chain reaction (qPCR) assays. The findings indicated that LP-YS3 remarkably decreased the disease activity index (DAI) of UC mice (p < .05), inhibited colon length shortening induced by UC, and elevated the value of colon weight/length ratio. LP-YS3 could also markedly reduce (p < .05) the activities of MDA, MPO, and NO; while an increase in the GSH content in the colonic tissue of UC mice. Moreover, LP-YS3 remarkably increased (p < .05) the serum level of IL-2 in UC mice, while reduced those of IL-10, IL-6, IL-1β, TNFα cytokines. qPCR data revealed that LP-YS3 could markedly upregulate the expression levels of c-Kit and SCF, while downregulate those of CXCR2 and IL-8 in the colonic tissue of UC mice (p < .05). LP-YS3 exerted an outstanding protective effect on DSS-induced colitis in C57BL/6J mice, especially at higher concentrations. Practical applications Lactobacillus plantarum YS3 is a newly isolated and identified lactic acid bacteria. This study confirmed that L. plantarum YS3 can inhibit colitis and has good probiotic potential, which needs further development and utilization.

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“…c-Kit gene, located on chromosome 4q12-13, belongs to proto-oncogene and its product is tyrosine kinase type III. As a receptor of SCF, c-Kit can regulate the proliferation and differentiation of hematopoietic stem cells through a series of signaling pathways [26,27]. SCF exerts a direct effect on inflammatory bowel disease by regulating the function and number of ICC.…”

Section: Discussionmentioning

confidence: 99%

Prophylactic Effect of Lactobacillus plantarum YS4 on Oxazolone‐Induced Colitis in BALB/c Mice

Tan

Suo

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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In the present research, the effects of Lactobacillus plantarum YS4 (LP-YS4) on colitis were tested in an oxazolone-induced mouse model. BALB/c mice were induced by oxazolone and then treated with LP-YS4. The serum levels of mice were analyzed using commercial kits and the protein and mRNA expression levels of mouse colon tissue were detected by Western blotting and qPCR assay, respectively. The results demonstrated that LP-YS4 significantly (P<0.05) increased the colon length and ratio of colon weight/length in mice with colitis and attenuated the negative effects of colitis. The results also showed that treatment with LP-YS4 significantly reduced the serum concentrations of ET-1, SP, and IL-10 while significantly increasing those of SS, VIP, and IL-2 in colitis mice (P<0.05). In addition, LP-YS4 significantly increased the activities of GSH and SOD while decreasing those of MPO and MDA in the colon tissue of colitis mice (P<0.05). LP-YS4 also significantly upregulated the mRNA and protein expression of c-Kit, eNOS, nNOSe, and SCF in colitis mice while significantly downregulating the relative expression of iNOS. In summary, LP-YS4 could reduce the negative effects of colitis, and such effects were better than those of the common probiotic Lactobacillus bulgaricus.

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Preventative effects of Lactobacillus plantarum YS-3 on oxazolone-induced BALB/c colitis in mice

Cited by 7 publications

References 18 publications

Preventive Effect of Lactobacillus acidophilus XY27 on DSS-Induced Ulcerative Colitis in Mice

Preventive Effect of Lactobacillus acidophilus XY27 on DSS-Induced Ulcerative Colitis in Mice

Protective effect of Lactobacillus plantarum YS3 on dextran sulfate sodium‐induced colitis in C57BL/6J mice

Prophylactic Effect of Lactobacillus plantarum YS4 on Oxazolone‐Induced Colitis in BALB/c Mice

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