Prevalent Latent Human Cytomegalovirus Genotype b2 in Biopsy Samples of Gastric Cancer

Abstract

Aim: Genotyping and prevalence of human cytomegalovirus (HCMV) in gastrointestinal tract remains unclear. Objective: Characterization of HCMV and its viral load in samples of patients with gastric cancer (GC) in northeast Iran. Methods: A total of 21 biopsies were collected from patients with GC. HCMV was detected and genotyped using multiplex nested PCR. Quantitative real-time PCR has been used to determine the viral load. Staining was performed to detect viral inclusions. Results: 76.19% of the samples had HCMV genotypic b2 with viral load <0.1 IU/μl. 66.67% of patients were positive for H. pylori. Conclusion: Multiplex nested-PCR method can detect CMV populations with low viral loads in GC biopsies. For the first time, it was determined that the genotype b2 of HCMV is prevalent in the gastrointestinal tract.

Cytomegalovirus Infection or Gastric Cancer? A Case Report and Review of the Literature

“…A multiplex nested PCR method can detect CMV populations with low viral loads in gastric cancer biopsies, and most of these populations include people with immunodeficiency (Mohebbi et al., 2020).…”

“…The prevalence of CMV infection in the stomach, and anywhere in the GI tract, needs to be investigated (Mohebbi et al, 2020). In one study, CMV-related gastric ulcer and gastroduodenitis were reported (Beany & Rainis, 2021).…”

Impact of umbelliprenin-containing niosome nanoparticles on VEGF-A and CTGF genes expression in retinal pigment epithelium cells

“…Internal glyceraldehyde 3-phosphate dehydrogenase (GAPDH) control was used to evaluate RNA extraction and cDNA synthesis. Real-time polymerase chain reaction (RT-PCR) evaluated the expression of VEGF-A, CTGF, and GAPDH genes [30] . The PCR primers that were obtained from our previous work are listed in Table 1 [9] .…”