Prevalência de paracoccidioidomicose-infecção determinada através de teste sorológico em doadores de sangue na região Noroeste do Paraná, Brasil

Abstract

In this paper the classical ELISA test was used, which is a highly sensitive method, with the objective of estimating the anti-Paracoccidioides brasiliensis antibodies level in healthy people, residing in supposedly endemic regions for PCM, Northwest Paran State, Brazil. The sera was analyzed from 680 blood donors' who were approved by the classical serologic screening by blood banks. Four cities were studied (Campo Mour o, Maring , Cianorte and Umuarama) with a sample of 170 donors each. The antigen used was Ag7days, diluted serum 1/400, anti human IgG-conjugate marked with peroxidase and revealed with O-Phenylenediamine. It was possible to detect 181 (27%) sera with antibody level above the cut off threshold. The percentage varied between the cities. These results allied with the favorable climatic and geographical conditions and also previous reports of PCM indigenous to the region, indicate that the Northwest of Paran is an endemic region for PCM.

Occurrence of 102 cases of paracoccidioidomycosis in 18 months in the Itaipu Lake region, Western Paraná

“…These data are known to differ with the postulates discussed in the literature 5,10 . News reports indicate the emergence of new epidemiological characteristics for P. brasiliensis 12 .…”

“…This mycosis is characterized as highly relevant to public health, its clinical manifestations result in irreversible physical disabilities that incapacitate the individual, usually in their most productive years 5 .…”

“…PCM mostly affects men of productive age, between 30 and 59 years-old 5,10,11 . Similarly, observation verified that the age group 30 to 59 years-old showed high prevalence in this study.…”

Pulmonary paracoccidoidomycosis: radiology and clinical-epidemiological evaluation

“…As a chronic systemic mycosis it carries a high morbidity and mortality, especially in cases of diffuse systemic involvement 14 or when associated with immune compromising conditions [15][16][17] , as also noticed with other endemic fungal diseases [18][19][20] . The prevalence of PCM in Brazil is estimated by regional serological surveys or epidemiological mortality reviews 2,[21][22][23] . PCM was the 8 th cause of mortality by chronic infectious diseases in Brazil, causing 3,181 deaths between 1980 and 1995.…”

Paracoccidioidomicose e tuberculose: diagnóstico diferencial

“…O teste de imunodifusão radial dupla (4) , exame sorológico de rotina, apresentou resultado negativo, porém teste de maior sensibilidade, o western blot, mostrou anticorpos contra o componente marcador da PCM (Figura 3). Além disso, a reação de ensaio imunossorvente ligado à enzima (ELISA) (10) também indicou para a presença de níveis elevados de anticorpos frente ao antígeno de P. brasiliensis TB; além disso, muitas vezes os laboratórios são pouco preparados para a confirmação laboratorial, tornando difícil o diagnóstico diferencial entre PCM e TB.…”