Prevalences of various hemoplasma species among cats in the United States with possible hemoplasmosis

Sykes, Jane E.; Terry, Jeralyn C.; Lindsay, LeAnn L.; Owens, Sean D.

doi:10.2460/javma.232.3.372

Cited by 79 publications

(134 citation statements)

References 16 publications

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“…6,14 Worldwide geographical variation in the prevalence of the different feline and canine hemoplasma species has been reported. 1,15,22 In Europe, prevalence studies with the use of polymerase chain reaction (PCR) for the diagnosis of hemoplasmas in cats have only been performed on domestic cats in Switzerland, Germany, northern Italy, and the United Kingdom. 2,5,9,19 Moreover, prevalence studies that use PCR for the diagnosis of M. haemocanis and 'Candidatus M. haematoparvum' have only been performed on domestic dogs in France, Switzerland, Japan, northern Tanzania, and Trinidad.…”

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“…?show "fnote_aff1"$^! "content-markup(./author-grp [1]/aff|./author-grp [1]/dept-list)> Three feline hemoplasma species exist: Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum', and 'Candidatus Mycoplasma turicensis', 8,10,15,20 whereas dogs can be infected by 2 hemoplasma species: Mycoplasma haemocanis and 'Candidatus Mycoplasma haematoparvum'. 1,7,14,18 The pathogenicity of hemoplasmas species varies from being asymptomatic to inducing severe hemolysis.…”

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Prevalence of Hemotropic Mycoplasmas in Healthy and Unhealthy Cats and Dogs in Spain

Roura

Peters

Altet³

et al. 2010

J VET Diagn Invest

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Abstract. The aims of the present study were to determine the prevalence of hemoplasmas in cats and dogs from the Barcelona area of Spain with the use of species-specific quantitative polymerase chain reaction (qPCR) assays and to evaluate any associations between hemoplasma infection, clinical presentation, and vector-borne infections. Blood samples from cats (191) and dogs (182) were included and were classified as healthy (149) or unhealthy (224). Ethylenediamine tetra-acetic acid blood samples underwent DNA extraction and qPCR analysis. Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum', and 'Candidatus Mycoplasma turicensis' were detected in cats, whereas Mycoplasma haemocanis and 'Candidatus Mycoplasma haematoparvum' were detected in dogs, with prevalences of 3.7%, 9.9%, 0.5%, 14.3%, and 0.6%, respectively. In cats, no association between hemoplasma infection and health status, age, breed, presence of anemia, Feline leukemia virus status, and other vector-borne infections was found, but outdoor access (P 5 0.009), male sex (P 5 0.01), and Feline immunodeficiency virus status (P , 0.001) were significantly associated with hemoplasma infection. In dogs, sex, age, health status, presence of anemia, and breed were not significantly associated with hemoplasma infection, but a significant association was found between hemoplasma infection and vector-borne infections (P , 0.001). The present report documents the occurrence of feline 'Candidatus M. turicensis' and canine 'Candidatus M. haematoparvum' infections in Spain.

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Prevalence of Hemotropic Mycoplasmas in Healthy and Unhealthy Cats and Dogs in Spain

Roura

Peters

Altet³

et al. 2010

J VET Diagn Invest

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“…At least 3 species infect cats: Mycoplasma haemofelis and ''Candidatus Mycoplasma haemominutum'', as well as a ''Candidatus Mycoplasma turicensis'', a new species that was first described in cats in Switzerland and subsequently detected in cats worldwide. 3,7,9,15,16 Mycoplasma haemofelis is capable of causing severe anemia in cats, whereas ''Ca. M. haemominutum'' has yet to be clearly associated with disease in immunocompetent cats.…”

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“…M. turicensis'' has never been cytologically identified. 9,10,12,14,15 The advent of polymerase chain reaction (PCR) technology has improved the ability to detect hemoplasmas. Recently, quantitative real-time PCR assays were used for specific detection and quantification of ''Ca.…”

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Use of Dried Blood Smears for Detection of Feline Hemoplasmas Using Real-Time Polymerase Chain Reaction

et al. 2008

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Abstract. The objective of the current study was to determine the sensitivity and specificity of real-time polymerase chain reaction (real-time PCR) for feline hemoplasmas when applied to DNA extracted from dried whole-blood smears in comparison to that for DNA extracted from liquid whole blood. Blood samples were collected into ethylenediamine tetra-acetic acid tubes from 305 cats with possible or suspected hemoplasmosis, and dried blood smears from each sample were prepared. DNA was extracted from blood smears and a 160-ml aliquot of each liquid blood sample by using a robotic extractor and was subjected to real-time PCR for feline glyceraldehyde-3-phosphate dehydrogenase (liquid blood), 18S ribosomal RNA (rRNA; dried blood), and ''Candidatus Mycoplasma haemominutum'', Mycoplasma haemofelis, and ''Candidatus Mycoplasma turicensis'' DNA. When using the results for liquid whole blood as the gold standard, the sensitivity of each assay for ''Ca. M. haemominutum'', M. haemofelis, and ''Ca. M. turicensis'' was 49 of 66 (74%), 11 of 13 (85%), and 11 of 20 (55%), respectively. The specificity of each assay was 224 of 234 (96%), 287 of 287 (100%), and 280 of 280 (100%), respectively. When possible, liquid blood samples should be submitted for detection of feline hemoplasmas by using real-time PCR. The improved sensitivity of real-time PCR on blood smears for M. haemofelis compared with that of the other hemoplasma species may reflect the higher organism burdens associated with infection with this species.

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Mycoplasma

Browning

Marenda

Markham

et al. 2010

Pathogenesis of Bacterial Infections in Animals

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Prevalences of various hemoplasma species among cats in the United States with possible hemoplasmosis

Abstract: Results supported the suggestion that these 3 hemoplasma species commonly occur among cats in the United States and that pathogenicity of the 3 species varies.

Cited by 79 publications

References 16 publications

Prevalence of Hemotropic Mycoplasmas in Healthy and Unhealthy Cats and Dogs in Spain

Prevalence of Hemotropic Mycoplasmas in Healthy and Unhealthy Cats and Dogs in Spain

Use of Dried Blood Smears for Detection of Feline Hemoplasmas Using Real-Time Polymerase Chain Reaction

Mycoplasma

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